<b>DNA barcode regions for differentiating <i>Cattleya walkeriana</i> and <i>C. loddigesii</i>

  • Hernando Rivera-Jiménez Universidade Estadual Paulista
  • Bruno César Rossini Universidade Estadual Paulista
  • Evandro Vagner Tambarussi Universidade Estadual Paulista / Universidade Estadual do Centro-Oeste
  • Elizabeth Ann Veasey Universidade de São Paulo
  • Bruna Ibanes Universidade de São Paulo
  • Celso Luis Marino Universidade Estadual Paulista

Resumo

 

Growers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. Thus, this study aimed to evaluate the feasibility of DNA barcode regions, namely ITS1, ITS2 and rpoC1, to discriminate between C. walkeriana and C. loddigesii species. DNA barcode regions were successfully amplified using primers designed to amplify plants. We also included sequences from public databases in order to test if these regions were able to discriminate C. walkeriana and C. loddigesii from other Cattleya species. These regions, and their combinations, demonstrated that the ITS1+ITS2 had the highest average interspecific distance (11.1%), followed by rpoC1 (1.06%). For species discrimination, ITS1+ITS2 provided the best results. The combined data set of ITS1+ITS2+rpoC1 also discriminated both species, but did not result in higher rates of discrimination. These results indicate that ITS region is the best option for molecular identification of these two species and from some other species of this genus.

 

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Publicado
2017-05-03
Como Citar
Rivera-Jiménez, H., Rossini, B. C., Tambarussi, E. V., Ann Veasey, E., Ibanes, B., & Marino, C. L. (2017). <b>DNA barcode regions for differentiating <i>Cattleya walkeriana</i> and <i>C. loddigesii</i&gt;. Acta Scientiarum. Biological Sciences, 39(1), 45-52. https://doi.org/10.4025/actascibiolsci.v39i1.33024
Seção
Genética

 

0.6
2019CiteScore
 
 
31st percentile
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0.6
2019CiteScore
 
 
31st percentile
Powered by  Scopus