Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365

José Ariévilo Gurgel Rodrigues, Edfranck de Sousa Oliveira Vanderlei, Ana Luíza Gomes Quinderé, Valdécio Silvano Monteiro, Silvânia Maria Mendes de Vasconcelos, Norma Maria Barros Benevides

Resumo


Caulerpa cupressoides var. lycopodium (Chlorophyta) contains three sulfated polysaccharides (SPs) fractions (SP1, SP2 and SP3); but, their pharmacological properties have been limited. We investigated the antinociceptive activity of non-anticoagulant fraction (SP1) and then its acute toxicological study in male Swiss mice was performed. Animals (19-25 g) received i.v. SP1 30 min. prior to injection 0.8% acetic acid (10 mL kg-1, i.p.); 1% formalin (20 µL, i.pl.) or were subjected to thermal stimuli. Open-field test was also performed. Mice were treated i.p. with SP1 or 0.9% saline (0.1 mL 10 g-1) for 72h. On the 4th day, the animals were anesthetized and sacrificed in order to collect blood and organs. SP1 (3, 9 or  27 mg kg-1) reduced (p < 0.05) the number of writhes induced by acetic acid by 44.21, 47.72 and 90.87%, respectively. SP1 inhibited (p < 0.05) the second phase of the formalin test, without antinociceptive effect in the hot-plate test, suggesting that its analgesic action occurs through of peripheral mechanisms. SP1 did not modify the locomotor activity. SP1 (27 mg kg-1) did not cause hepatic or renal dysfunctions, but affected the spleen of animals (p < 0.05). Therefore, SP1 has analgesic action with high tolerance by the animals, presenting its potential applicability in pain conditions.

 

 


Palavras-chave


green alga; sulfated sugars; nociception; biochemical analysis

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DOI: http://dx.doi.org/10.4025/actascitechnol.v35i3.15365





ISSN 1806-2563 (impresso) e ISSN 1807-8664 (on-line) e-mail: actatech@uem.br

  

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