Extraction of total polyphenols from hibiscus (Hibiscus sabdariffa L.) and waxweed / ‘sete-sangrias’ (Cuphea carthagenensis) and evaluation of their antioxidant potential

Daniele Begmeier, Pedro Henrique Dalberto Berres, Débora Filippi, Denise Bilibio, Vanderlei Rodrigo Bettiol, Wagner Luiz Priamo


Current research investigates the extraction process of total polyphenols from hibiscus (Hibiscus sabdariffa L.) and waxweed (Brazilian name: ‘sete-sangrias’) (Cuphea carthagenensis) and evaluates the antioxidant potential of their extracts. The extraction stage comprised investigation on the following parameters: i) solvents (acetone and ethanol) pure and fractioned with water; ii) variables (temperature, stirring, solvent ratio, time and pH). Total polyphenols were quantified by Folin-Ciocalteau reagent and antioxidant activity was determined by ABTS•+ (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. Results showed that, depending on experimental conditions, total phenolic contents for hibiscus and waxweed ranged between 460.86 mg GAE 100 g-1 and 5012.54 mg GAE 100 g-1 and between 462.86 mg GAE 100 g-1 and 4215.99 mg GAE 100 g-1, respectively. Waxweed had a higher antioxidant activity when compared to that of hibiscus by both ABTS•+ and DPPH. Data showed that hibiscus and waxweed have a significant amount of polyphenols which may be extracted in mild processing conditions and then employed as natural antioxidant sources in industrial processes.


polyphenolic fraction; DPPH; natural extracts

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DOI: http://dx.doi.org/10.4025/actascitechnol.v36i3.19093

ISSN 1806-2563 (impresso) e ISSN 1807-8664 (on-line) e-mail: actatech@uem.br


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