Cryopreservation of semen of Thailand tilapia (Oreochromis spp.) fed diet with different oil sources

Rodrigo Diana Navarro, Fernanda Keley Silva Pereira Navarro, Viviane Oliveira Felizardo, Luis David Solis Murgas, Marcelo Maia Pereira


This study evaluated the quality of sperm cryopreserved with dimethylsulfoxide (DMSO) or methanol, of Thailandtilapia (Oreochromis spp.) fed diet with different oilsources. eightyThailand tilapia fed different oil sources (T1: linseed oil, T2: soybean oil, T3: fish oil, T4: corn oil) and were analyzed as for motility rate, and duration of motility in ‘fresh sperm’ and after cryopreservation. No significant difference was found for the final weight, total length, rate and duration of motility of fresh sperm in the different treatments. Significant differences were observed between fish, corn and linseed oil treatments on the rate of motility relative to other different oil sources with the cryoprotectant DMSO prior to freezing. After thawing, fish fed diet supplemented with fish oil presented a significantly higher rate and duration of sperm motility. Nevertheless, for better reproductive parameters after freezing, it is recommended the use of fish oil associated with cryoprotectant methanol.



freeze; cryoprotectant; DMSO; methanol

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ISSN 1806-2563 (impresso) e ISSN 1807-8664 (on-line) e-mail:


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