<b>Estimation of genetic distance among genotypes of caraway (<i>Carum carvi</i> L.) using RAPD-PCR

  • Katarzyna Seidler-Łożykowska Institute of Natural Fibres and Medicinal Plants
  • Anetta Kuczyńska Polish Academy of Sciences
  • Katarzyna Mikołajczyk Plant Breeding and Acclimatization Institute
  • Joanna Nowakowska Plant Breeding and Acclimatization Institute
  • Jan Bocianowski Poznań University of Life Sciences

Resumo

In order to estimate genetic diversity among starting materials and breeding strains of caraway, a collection of 17 accessions from botanical gardens in Europe, two cultivars ‘Rekord’ and ‘Kończewicki’, and four own breeding strains were analyzed by RAPD-PCR. The representative samples, each of five individual plants of accession, cultivar or strain, were taken from young rosette leaves. Forty of Genset Oligos RAPD primers were used for analysis and eight of them produced clear and reproducible banding patterns. In total, 62 banding patterns were obtained revealing 23 polymorphic bands, whereas the number of polymorphic bands ranged from two to four for one primer. The GS12 and GS43 primers generated two polymorphic bands, while each of the GS8, GS21, GS22, GS41 and GS53 primers generated three bands. The GS53 primer was the most informative one, revealing 60% of the estimated polymorphism. The estimated value of genetic distance ranged from 0.22 to 0.67. The lowest genetic distance was found between accessions from Cluj and Lousanne (0.22). The highest genetic distance was estimated between accession fromBerlinand the strain no. 6 of cultivar ‘Kończewicki’ (0.67). UPGMA cluster analysis, based on eight RAPD primers, categorized the analyzed genotypes into four groups.

 

Downloads

Não há dados estatísticos.
Publicado
2014-04-29
Como Citar
Seidler-Łożykowska, K., Kuczyńska, A., Mikołajczyk, K., Nowakowska, J., & Bocianowski, J. (2014). <b>Estimation of genetic distance among genotypes of caraway (<i>Carum carvi</i&gt; L.) using RAPD-PCR. Acta Scientiarum. Agronomy, 36(2), 183-188. https://doi.org/10.4025/actasciagron.v36i2.18197
Seção
Genética e Melhoramento

 

2.0
2019CiteScore
 
 
60th percentile
Powered by  Scopus

 

2.0
2019CiteScore
 
 
60th percentile
Powered by  Scopus