Somatic embryogenesis from mature split seeds of jaboticaba (Plinia peruviana (Poir) Govaerts)
Resumo
Plinia peruviana is a species that is native to Brazil and is important due to the taste and medicinal properties of its fruits. Young leaves and split mature seeds were used as explants to initiate somatic embryogenesis to obtain a large number of plants in a short period of time. Leaf discs were cultured in MS medium containing various concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid) or picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid). In the case of the mature seeds, various concentrations of glutamine, 2,4-D and a combination of auxin and BAP (6-benzylaminopurine) were tested for somatic embryogenesis induction. For somatic embryo maturation, several concentrations of PEG 6000 (polyethylene glycol; up to 90 g L-1) were tested. After 60 days of culture using leaf discs, callus formation occurred in all treatments, with the highest averages obtained with 10 μM 2,4-D. However, these calluses did not form somatic embryos. For the cultured seeds, the best treatment was the MS medium with 1,000 mg L-1 glutamine and 10 μM 2,4-D without BAP. The supplementation of 60 g L-1 PEG 6000 was sufficient to promote the maturation of the somatic embryos. Histological analyses of the calluses that were formed from leaf discs showed nonembryogenic characteristics. In contrast, the calluses that originated from mature seeds had small and round cells with little vacuolation, which are characteristics of embryogenic structures.
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Referências
Akhtar, N. (2010). Evaluation of the efficiency of somatic embryogenesis in guava (Psidium guajava L.). The Journal of Horticultural Science and Biotechnology, 85(6), 556-562. DOI: 10.1080/14620316.2010.11512714
Attree, S. M., & Fowke, L. C. (1993). Embryogeny of gymnosperms: advances in synthetic seed technology of conifers. Plant Cell, Tissue and Organ Culture, 35(1), 1-35. DOI: 10.1007/BF00043936
Bajpai, A., Kalim, S., Chandra, R., & Kamle, M. (2016). Recurrent somatic embryogenesis and plant regeneration in Psidium guajava L. Brazilian Archives of Biology and Technology, 59(1), 1-12. DOI: 10.1590/1678-4324-2016150170
Cangahuala-Inocente, G. C., Silveira, V., Caprestano, C. A., Floh, E. I. S., & Guerra, M. P. (2014). Dynamics of physiological and biochemical changes during somatic embryogenesis of Acca sellowiana. In Vitro Cellular & Developmental Biology - Plant, 50(2), 166-175. DOI: 10.1007/s11627-013-9563-3
Canhoto, J. M., & Cruz, G. S. (1996). Histodifferentiation of somatic embryos in cotyledons of pineapple guava (Feijoa sellowiana Berg). Protoplasma, 191(1), 34-45. DOI: 10.1007/BF01280823
Canhoto, J. M., & Cruz, G. S. (1994). Improvement of somatic embryogenesis in Feijoa sellowiana Berg (Myrtaceae) by manipulation of culture media composition. In Vitro Cellular & Developmental Biology - Plant, 30(1), 21-25. DOI: 10.1007/BF02632115
Canhoto, J. M., Lopes, M. L., & Cruz, G. S. (1999). Somatic embryogenesis and plant regeneration in myrtle (Myrtaceae). Plant Cell, Tissue and Organ Culture, 57(1), 13-21. DOI: 10.1023/A:1006273128228
Carlsson, J., Svennerstam, H., Moritz, T., Egertsdotter, U., & Ganeteg, U. (2017). Nitrogen uptake and assimilation in proliferating embryogenic cultures of Norway spruce – Investigating the specific role of glutamine. PLoS ONE, 12(8), 1-18. DOI: 10.1371/journal.pone.0181785
Cassol, D. A., Wagner Júnior, A., Pirola, K., Dotto, M., & Citadin, I. (2015). Embalagem, época e ácido indolbutírico na propagação de jabuticabeira por alporquia. Revista Brasileira de Fruticultura, 37(1), 267-272. DOI: 10.1590/0100-2945-011/14
Corredoira, E., San-José, M. C., & Vieitez, A. M. (2012). Induction of somatic embryogenesis from different explants of shoot cultures derived from young Quercus alba trees. Trees, 26(3), 881-891. DOI: 10.1007/s00468-011-0662-7
Cruz, G. S., Canhoto, J. M., & Abreu, M. A. V. (1990). Somatic embryogenesis and plant regeneration from zygotic embryos of Feijoa sellowiana Berg. Plant Science, 66, 262-270. DOI: 10.1016/0168-9452(90)90212-7
Dal Vesco, L. L., & Guerra, M. P. (2001). The effectiveness of nitrogen sources in Feijoa somatic embryogenesis. Plant Cell, Tissue and Organ Culture, 64(1), 19-25. DOI: 10.1023/A:1010635926146
De-la-Peña, C., Nic-Can, G. I., Galaz-Ávalos, R. M., Avilez-Montalvo, R., & Loyola-Vargas, V. M. (2015). The role of chromatin modifications in somatic embryogenesis in plants. Frontiers in Plant Science, 6(635), 1-15. DOI: 10.3389/fpls.2015.00635
Duarte, O., & Paull, R. E. (2015). Myrtaceae. In O. Duarte, & R. E. Paull (Eds.), Exotic fruits and nuts of the new world (p. 51-95). Wallingford, UK: CABI Publishing.
Fehér, A. (2005). Why somatic plant cells start to form embryos? In A. Mujib, & J. Samaj (Eds.), Somatic embryogenesis, vol. 2, Plant cell monographs (p. 85-101). Heidelberg, GE: Springer.
Fraga, H. P. F., Vieira, L. N., Caprestano, C. A., Steinmacher, D. A., Micke, G. A., Spudeit, D. A., … Guerra, M. P. (2012). 5-Azacytidine combined with 2,4-D improves somatic embryogenesis of Acca sellowiana (O. Berg) Burret by means of changes in global DNA methylation levels. Plant Cell Reports, 31(12), 2165-2176. DOI: 10.1007/s00299-012-1327-8
Guerra, M. P., Cangahuala-Inocente, G. C., Dal Vesco, L. L., Pescador, R., & Caprestano, C. A. (2013). Micropropagation systems in Feijoa (Acca sellowiana (O. Berg) Burret). In M. Lambardi, E. A. Ozudogru, & S. M. Jain (Eds.), Protocols for micropropagation of select economically-important horticultural plants (p. 45-62). New York, US: Human Press.
Guerra, M. P., Dal Vesco, L. L., Ducroquet, J. P. H. J., Nodari, R. O., & Dos Reis, M. S. (2001). Somatic embryogenesis in goiabeira serrana: genotype response, auxinic shock and synthetic seeds. Revista Brasileira de Fisiologia Vegetal, 13(2), 117-128. DOI: 10.1590/S0103-31312001000200001
Gutiérrez-Mora, A., González-Gutiérrez, A. G., Rodríguez-Garay, B., Ascencio-Cabral, A., & Li-Wei, L. (2012). Plant somatic embryogenesis: some useful considerations. In K. Sato (Ed.), Embryogenesis (p. 229-248). London: UK, InTech.
Isah, T. (2016). Induction of somatic embryogenesis in woody plants. Acta Physiologiae Plantarum, 38(5), 1-22. DOI: 10.1007/s11738-016-2134-6
Jalali, M. A., Sirmandi, H. B., & Hatamzadeh, A. (2017). Effects of carbohydrate source and polyethylene glycol on maturation and germination of somatic embryos in walnut (Juglans regia L.). Journal of Crop Science and Biotechnology, 20(1), 29-35. DOI: 10.1007/s12892-016-00089-1
Joshi, R., & Kumar, P. (2013). Regulation of somatic embryogenesis in crops: a review. Agricultural Reviews, 34(1), 1-20.
Karnovsky, M. J. (1965). A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. Journal of Cell Biology, 27(2), 137-138.
Kumar, V., & Van Staden, J. (2017). New insights into plant somatic embryogenesis: an epigenetic view. Acta Physiologiae Plantarum, 39(194), 1-17. DOI: 10.1007/s11738-017-2487-5
Lloyd, G., & McCown, B. H. (1980). Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture. Combined Proceedings International Plant Propagators' Society, 30, 421-427.
LoSchiavo, F., Pitto, L., Giuliano, G., Torti, G., Nuti-Ronchi, V., Marazziti, D., ... Terzi, M. (1989). DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs. Theoretical and Applied Genetics, 77, 325-331. DOI: 10.1007/BF00305823
Merkle, S. A., Parrott, W. A., & Flinn, B. S. (1995). Morphogenic aspects of somatic embryogenesis. In T. A. Thorpe (Ed.), In vitro embryogenesis in plants (p. 155-204). Dordrecht, NE: Kluwer Academic Publishers.
Misra, S., Attree, S. M., Leal, I., & Fowke, L. C. (1993). Effect of abscisic acid, osmoticum and desiccation on synthesis of storage proteins during the development of white spruce somatic embryos. Annals of Botany, 71(1), 11-22. DOI: 10.1006/anbo.1993.1002
Möller, B., & Weijers, D. (2009). Auxin control of embryo patterning. Cold Spring Harbor Perspectives in Biology, 1(5), 1-13. DOI: 10.1101/cshperspect.a001545
Motoike, S. Y., Saraiva, E. S., Ventrella, M. C., Silva, C. V., & Salomão, L. C. C. (2007). Somatic embryogenesis of Myrciaria aureana (Brazilian grape tree). Plant Cell, Tissue and Organ Culture, 89, 75-81. DOI: 10.1007/s11240-007-9210-y
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15, 473-497. DOI: 10.1111/j.1399-3054.1962.tb08052.x
Nolan, K. E., & Rose, R. J. (2010). Plant regeneration – somatic embryogenesis. In M. R. Davey, & P. Anthony (Eds.), Plant cell culture (p. 39-60). Chichester, UK: Wiley-Blackwell.
Pence, V. C. (1992). Abscisic acid and the maturation of cacao embryos in vitro. Plant Physiology, 98(4), 1391-1395. DOI: 10.1104/pp.98.4.1391
Pescador, R., Kerbauy, G. B., Fraga, H. P. F., Hamasaki, R. M., Tavares, L. B. B., & Guerra, M. P. (2012). Dynamics of free and 3H-labelled glutamine concentrations during zygotic and somatic embryogenesis of Feijoa [Acca sellowiana (O. Berg.) Burret]. Journal of Horticultural Science and Biotechnology, 87(6), 583-587. DOI: 10.1080/14620316.2012.11512915
Quiroz-Figueroa, F. R., Rojas-Herrera, R., Galaz-Avalos, R. M., & Loyola-Vargas, V. M. (2006). Embryo production through somatic embryogenesis can be used to study cell differentiation in plants. Plant Cell, Tissue and Organ Culture, 86(3), 285-301. DOI: 10.1007/s11240-006-9139-6
Rai, M. K., Akhtar, N., & Jaiswal, V. S. (2007). Somatic embryogenesis and plant regeneration in Psidium guajava L. cv. Banarasi local. Scientia Horticulturae, 113, 129-133. DOI: 10.1016/j.scienta.2007.02.010
Rancillac, M., Klinguer, A., Klinguer, S., & Millet, B. (1996). Preliminary investigations on somatic embryogenesis from leaf discs of red oak (Quercus rubra L.). Plant Growth Regulation, 20(1), 67-73. DOI: 10.1007/BF00024061
Rudiyanto, R., Efendi, D., & Ermayanti, T. M. (2014). Somatic embryo germination of Jatropha curcas L in presence of sucrose and poly ethylene glycol (PEG). Annales Bogorienses, 18(1), 35-43. DOI: 10.14203/ann.bogor.2014.v18.n1.35-43.
Shang, H., Liu, C., Zhang, C., Li, F., Hong, W., & Li, F. (2009). Histological and ultrastructural observation reveals significant cellular differences between Agrobacterium transformed embryogenic and non-embryogenic calli of cotton. Journal of Integrative Plant Biology, 51(5), 456-465. DOI: 10.1111/j.1744-7909.2009.00824.x
Souza-Moreira, T. M., Moreira, R. D., Sacramento, L. V. S., & Pietro, R. C. L. R. (2008). Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves. Revista Brasileira de Farmacognosia, 20(1), 48-53. DOI: 10.1590/S0102-695X2010000100011
Tholakalabavi, A., Zwiazek, J. J., & Thorpe, T. A. (1994). Effect of mannitol and glucose-induced osmotic stress on growth, water relations and solute composition of cell suspension cultures of poplar (Populus deltoides var. occidentalis) in relation to anthocyanin accumulation. In Vitro Cellular and Developmental Biology, 30, 164-170. DOI: 10.1007/BF02632208
Von Arnold, S. (2008). Somatic embryogenesis (3rd ed.). In E. F. George, M. A. Hall, & G. J. De Klerk (Eds.), Plant propagation by tissue culture (p. 335-354). Dordrecht, NE: Springer.
Von Arnold, S., Sabala, I., Bozhkov, P., Dyachok, J., & Filonova, L. (2002). Developmental pathways of somatic embryogenesis. Plant Cell, Tissue and Organ Culture, 69(3), 233-249. DOI: 10.1023/A:1015673200621
Welander, M. (1988). Plant regeneration from leaf and stem segments of shoots raised in vitro from mature apple trees. Journal of Plant Physiology, 132(6), 738-744. DOI: 10.1016/S0176-1617(88)80238-4
Wu, S. B., Dastmalchi, K., Long, C., & Kennelly, E. J. (2012). Metabolite profiling of jaboticaba (Myrciaria cauliflora) and other dark-colored fruit juices. Journal of Agricultural and Food Chemistry, 60(30), 7513-7525. DOI: 10.1021/jf301888y
Wu, S. B., Long, C., & Kennelly, E. J. (2013). Phytochemistry and health benefits of jaboticaba, an emerging fruit crop from Brazil. Food Research International, 54(1), 148-159. DOI: 10.1016/j.foodres.2013.06.021
Yang, X., & Zhang, X. (2010). Regulation of somatic embryogenesis in higher plants. Critical Reviews in Plant Science, 29(1), 36-57. DOI: 10.1080/07352680903436291
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