Lignin content, morphoanatomical traits and antioxidant activity as determinants of soybean seed storage tolerance and quality
Resumo
The storage of soybean seeds is a critical aspect of the production process, making it essential for genotypes to show tolerance during this phase to maintain seed quality. This study aimed to examine and correlate lignin content in the seed coat, enzyme activity expression, and morphoanatomical characteristics of soybean cultivars with the preservation of physiological quality and storage tolerance. Eight soybean cultivars were selected, produced, and harvested under uniform edaphoclimatic conditions. Physiological quality assessments were conducted at six storage intervals: 0, 60, 120, 180, 240, and 360 days. Evaluations included tests for germination, emergence, accelerated aging, electrical conductivity, and lignin content in the seed coat. Additionally, biochemical and enzymatic analyses of antioxidant metabolism and morphoanatomical examinations were performed using light microscopy on seeds stored for 0 and 360 days. The experiment used an 8 × 6 factorial arrangement, encompassing eight cultivars and six storage periods. For the enzymatic and morphoanatomical analyses, a 4 × 2 factorial design was employed, involving four cultivars and two storage durations. The results showed that genotype significantly influenced the tolerance of soybean seeds to extended storage periods. Seeds proving high physiological quality and storage resilience exhibited reduced hydrogen peroxide accumulation and diminished lipid peroxidation. Furthermore, morphoanatomical analyses provide a promising method for selecting genotypes with enhanced physiological quality and storage tolerance.
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Abati, J., Zucareli, C., Brzezinski, C. R., Krzyzanowski, F. C., França-Neto, J. B., & Henning, F. A. (2021a). Metabolites of the phenylpropanoid pathway and physiological quality of soybean seeds in storage. Journal of Seed Science, 43, 1-14. https://doi.org/10.1590/2317-1545v43253585
Abati, J., Zucareli, C., Brzezinski, C. R., Lopes, I. O. N., Krzyzanowski, F. C., Moraes, L. A. C., & Henning, F. A. (2021b). Water absorption and storage tolerance of soybean seeds with contrasting seed coat characteristics. Acta Scientiarum. Agronomy, 44(1), 1-10. https://doi.org/10.4025/actasciagron.v44i1.53096
Abati, J., Zucareli, C., Brzezinski, C. R., Moraes, L. A. C., Lopes, I. O. N., Mertz-Henning, L. M., Krzyzanowski, F. C., & Henning, F. A. (2022). Physiological potential and antioxidant metabolism during storage of soybean seeds contrasting with phenylpropanoid pathway compounds. Bragantia, 81, 1-14. https://doi.org/10.1590/1678-4499.20210319
Azevedo, R. A., Alas, R. M., Smith, R. J., & Lea, P. J. (1998). Response of antioxidant enzymes to transfer from elevated carbon dioxide to air and ozone fumigation, in the leaves and roots of wild‐type and a catalase‐deficient mutant of barley. Physiologia Plantarum, 104(2), 280–292. https://doi.org/10.1034/j.1399-3054.1998.1040217.x
Batista, E. C., Villela, G. M., Pires, R. M. O., Santos, H. O., Carvalho, E. R., & Bruzi, A. T. (2022). Physiological quality of soybean seeds and the influence of maturity group. Journal of Seed Science, 44, 1-11. https://doi.org/10.1590/2317-1545v44261325
Buege, J. A., & Aust, S. D. (1978). Microsomal lipid peroxidation. In Methods in Enzymology (v. 52) (pp. 302-310). Elsevier. https://doi.org/10.1016/S0076-6879(78)52032-6
Carvalho, E. R., Mavaieie, D. P. R., Oliveira, J. A., Carvalho, M. V., & Vieira, A. R. (2014a). Alterações isoenzimáticas em sementes de cultivares de soja em diferentes condições de armazenamento. Pesquisa Agropecuária Brasileira, 49(12), 967-976. https://doi.org/10.1590/S0100-204X2014001200007
Carvalho, E. R., Oliveira, J. A., & Caldeira, C. M. (2014b). Qualidade fisiológica de sementes de soja convencional e transgênica RR produzidas sob aplicação foliar de manganês. Bragantia, 73(3), 219-228. https://doi.org/10.1590/1678-4499.0096
Carvalho, E. R., Oliveira, J. A., Reis, L. V., & Ferreira, T. F. (2015). Mn foliar sobre a qualidade sanitária e lignina de sementes de soja convencional e resistente ao glifosato. Revista Ciência Agronômica, 46(1), 135-143. https://doi.org/10.1590/S1806-66902015000100016
Carvalho, E. R., Oliveira, J. A., Mavaieie, D. P. R., Silva, H. W., & Lopes, C. G. M. (2016). Pre-packing cooling and types of packages in maintaining physiological quality of soybean seeds during storage. Journal of Seed Science, 38(2), 129-139. https://doi.org/10.1590/2317-1545v38n2158956
Castro, D. G., Bruzzi, A. T., Zambiazzi, E. V., Rezende, P. M., Zuffo, A. M., Sales, A. P., Soares, I. O., Borges, I. M. M., & Bianchi, M. C. (2019). Qualidade fisiológica e expressão enzimática de sementes de soja RR®. Revista de Ciências Agrárias, 40(1), 222-235. https://doi.org/10.19084/RCA16054
Castro, E. M., Pinho, É. V. R. V., Lima, A. E., Labory, C. R. G., Santos, H. O., Alves, E., & Guerra, A. F. R. (2024). Physiological quality, lignin and the ultrastructural characterization of soybean seeds. Acta Scientiarum. Agronomy, 46(1), 1-12. https://doi.org/10.4025/actasciagron.v46i1.63621
Gris, C. F., Pinho, E. V. R. V., Andrade, T., Baldoni, A., & Carvalho, M. L. M. (2010). Qualidade fisiológica e teor de lignina no tegumento de sementes de soja convencional e transgênica RR submetidas a diferentes épocas de colheita. Ciência e Agrotecnologia, 34(2), 374-381. https://doi.org/10.1590/S1413-70542010000200015
Hernández, J. A., Jiménez, A., Mullineaux, P., & Sevilia, F. (2000). Tolerance of pea (Pisum sativum L.) to long‐term salt stress is associated with induction of antioxidant defences. Plant, Cell & Environment, 23(8), 853-862. https://doi.org/10.1046/j.1365-3040.2000.00602.x
International Seed Testing Association [ISTA]. (2024). International rules for seed testing. ISTA. https://doi.org/10.15258/istarules.2024.F
Krzyzanowski, F. C., França-Neto, J. B., Gomes-Junior, F. G., & Nakagawa, J. (2020). Testes de vigor baseados em desempenho de plântulas. In F. C. Krzyzanowski, R. D. Vieira, J. B. França-Neto, & J. Marcos-Filho (Eds.), Vigor de sementes: conceitos e testes (2. ed.), (pp. 79–140). ABRATES.
Krzyzanowski, F. C., França-Neto, J. B., & Henning, F. A. (2023). Importance of the lignin content in the pod wall and seed coat on soybean seed physiological and health performances. Journal of Seed Science, 45, 1-19. https://doi.org/10.1590/2317-1545v45268562
Madureira, A., Molinari, M. D. C., Marin, S. R. R., Pagliarini, R. F., Henning, F. A., Nepomuceno, A. L., Finatto, T., & Mertz-Henning, L. M. (2023). Correlations between lignin content and related genes, weathering deterioration, and soybean seed quality at pre-harvest. Journal of Crop Science and Biotechnology, 26, 489-497. https://doi.org/10.1007/s12892-023-00193-5
Marcos-Filho, J. (2015). Seed physiology of cultivated plants. ABRATES.
Marcos-Filho, J. (2020). Teste de envelhecimento acelerado. In F. C. Krzyzanowski, R. D. Vieira, J. B. França-Neto, & J. Marcos-Filho (Eds.), Vigor de sementes: conceitos e testes (2. ed.) (pp. 79-140). ABRATES.
Matera, T. C., Pereira, L. C., Braccini, A. L., Krzyzanowski, F. C., Scapim, C. A., Piana, S. C., Marteli, D. C. V., Pereira, R. C., Ferri, G. C., & Suzukawa, A. K. (2019). Accelerated aging test and its relationship to physiological potential of soybean seeds. Journal of Seed Science, 41(3), 301-308. https://doi.org/10.1590/2317-1545v41n3212746
Mathias, V., Coelho, C. M. M., & Garcia, J. (2019). Soluble protein as indicative of physiological quality of soybean seeds. Revista Caatinga, 32(3), 730-740. https://doi.org/10.1590/1983-21252019v32n317rc
Mavaieie, D. P. R., Carvalho, E. R., Ferreira, V. F., Oliveira, J. A., Ferreira, T. F., & Reis, L. V. (2019). Performance of treated seeds of different soybean cultivars in function of environments and storage periods. Revista de Agricultura, 94(3), 179-195. https://doi.org/10.37856/bja.v94i3.3243
Mertz-Henning, L. M., Nagashima, A. I., Krzyzanowski, F. C., Binneck, E., & Henning, F. A. (2015). Relative quantification of gene expression levels associated with lignin biosynthesis in soybean seed coat. Seed Science and Technology, 43(3), 445-455. https://doi.org/10.15258/sst.2015.43.3.13
Min, C. W., Lee, S. H., Cheon, Y. E., Han, W. Y., Ko, J. M., Kang, H. W., Kim, Y. C., Agrawal, G. K., Rakwal, R., Gupta, R., & Kim, S. T. (2017). In-depth proteomic analysis of Glycine max seeds during controlled deterioration treatment reveals a shift in seed metabolism. Journal of Proteomics, 169, 125-135. https://doi.org/10.1016/j.jprot.2017.06.022
Moreira-Vilar, F. C., Siqueira-Soares, R. D. C., Finger-Teixeira, A., Oliveira, D. M., Ferro, A. P., Rocha, G. J., Ferrarese, M. L. L., Santos, W. D., & Ferrarese-Filho, O. (2014). The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods. PLoS ONE, 9(10), 1-7. https://doi.org/10.1371/journal.pone.0110000
Moreno, K. A. A., Pires, R. M. O., Castro, M. L. R., Vasconcellos, R. C. C., Santos, H. O., & Pinho, É. V. R. V. (2019). Gene expression related to physiological quality of soybean seeds. Journal of Agricultural Science, 11(3), 370-380. https://doi.org/10.5539/jas.v11n3p370
Nakada, P. G., Oliveira, J. A., Melo, L. C., Gomes, L. A. A., & Pinho, É. V. R. V. (2011). Desempenho fisiológico e bioquímico de sementes de pepino nos diferentes estádios de maturação. Revista Brasileira de Sementes, 33(1), 113-122. https://doi.org/10.1590/S0101-31222011000100013
Pinheiro, D. T., Dias, D. C. F. S., Medeiros, A. D., Ribeiro, J. P. O., Silva, F. L., & Silva, L. J. (2021). Weathering deterioration in pre-harvest of soybean seeds: Physiological, physical, and morpho-anatomical changes. Scientia Agricola, 78(Suppl. 1), 1-10. https://doi.org/10.1590/1678-992x-2020-0166
Pirredda, M., Fañanás-Pueyo, I., Oñate-Sánchez, L., & Mira, S. (2023). Seed longevity and ageing: A review on physiological and genetic factors with an emphasis on hormonal regulation. Plants, 13(1), 1-21. https://doi.org/10.3390/plants13010041
R Core Team. (2022). R: A language and environment for statistical computing. R Foundation for Statistical Computing. https://www.R-project.org/
Rao, P. J. M., Pallavi, M., Bharathi, Y., Priya, P. B., Sujatha, P., & Prabhavathi, K. (2023). Insights into mechanisms of seed longevity in soybean: A review. Frontiers in Plant Science, 14, 1-13. https://doi.org/10.3389/fpls.2023.1206318
Ribeiro, A. C., Guimarãez, P. T., & Alvarez, V. H. (1999). Recomendação para o uso de corretivos e fertilizantes para o Estado de Minas Gerais. 5a aproximação. CFSEMG.
Rosa, D. P., Nobre, D. A. C., Oliveira, D. S., Silva, F. C. S., Bezerra, A. R. G., Silva, A. F., & Sediyama, T. (2017). Genetic diversity in soybean seed quality under different storage conditions. Semina: Ciências Agrárias, 38(1), 57-72. https://doi.org/10.5433/1679-0359.2017v38n1p57
Ruppin, N. W., Terra, L. E. M., Fernandes, T. O. M., Alburquerque, C. J. B., & Brandão Junior, D. S. (2019). Caracterização morfofsiológica de sementes de diferentes cultivares de soja armazenadas sob condições não controladas. Caderno de Ciências Agrárias, 11, 1-8. https://doi.org/10.35699/2447-6218.2019.14731
Schons, A., Silva, C. M., Pavan, B. E., Silva, A. V., & Mielezrski, F. (2019). Respostas do genótipo, tratamento de sementes e condições de armazenamento no potencial fisiológico de sementes de soja. Revista de Ciências Agrárias, 41(1), 109-121. https://doi.org/10.19084/RCA17183
Sharma, S., Kaur, A., Bansal, A., & Gill, B. S. (2013). Positional effects on soybean seed composition during storage. Journal of Food Science and Technology, 50(2), 353-359. https://doi.org/10.1007/s13197-011-0341-0
Smaniotto, T. A. S., Resende, O., Marçal, K. A. F., Oliveira, D. E. C., & Simon, G. A. (2014). Qualidade fisiológica das sementes de soja armazenadas em diferentes condições. Revista Brasileira de Engenharia Agrícola e Ambiental, 18(4), 446-453. https://doi.org/10.1590/S1415-43662014000400013
Thirusendura-Selvi, D., & Saraswathy, S. (2018). Seed viability, seed deterioration and seed quality improvements in stored onion seeds: A review. The Journal of Horticultural Science and Biotechnology, 93(1), 1-7. https://doi.org/10.1080/14620316.2017.1343103
Velikova, V., Yordanov, I., & Edreva, A. (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Plant Science, 151(1), 59-66. https://doi.org/10.1016/S0168-9452(99)00197-1
Vieira, R. D., & Marcos-Filho, J. (2020). Teste de condutividade elétrica. In F. C. Krzyzanowski, R. D. Vieira, J. B. França-Neto, & J. Marcos-Filho (Eds.), Vigor de sementes: conceitos e testes (2. ed.) (pp. 333-388). ABRATES.
Copyright (c) 2026 Amanda Carvalho Penido Nardelli, Venicius Urbano Vilela Reis, Genaina Aparecida de Souza, Raquel Maria de Oliveira Pires, Debora Kelli Rocha, Everson Reis Carvalho (Autor)
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Funding data
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Conselho Nacional de Desenvolvimento Científico e Tecnológico
Grant numbers 314408/2021-5 -
Fundação de Amparo à Pesquisa do Estado de Minas Gerais
Grant numbers APQ-01869-18
