Genetic approaches to develop bacterial wilt resistant tobacco cultivars
DOI:
https://doi.org/10.4025/actasciagron.v48.i1.76997Palavras-chave:
plant disease resistance; Ralstonia solanacearum; recurrent selection.Resumo
One of the most notorious pathogens affecting tobacco production is tobacco bacterial wilt (TBW), caused by Ralstonia solanacearum. Controlling TBW is a global issue, and developing more resistant cultivars is one of the solutions. Therefore, this study investigated the effectiveness of recurrent selection (RS) as a breeding strategy to enhance resistance to R. solanacearum in tobacco. We also aimed to identify the appropriate progeny type for RS. The trials were carried out in an area where the pathogen is known to occur, with seedlings inoculated with R. solanacearum to assure the presence of the pathogen. The incidence of the pathogen was evaluated over two seasons. In the first year, we utilized 100 S1:2 progeny from the original population (cycle 0) and 108 half-sib progeny of cycle I. In the subsequent year, we evaluated 196 S0:1 progeny of CI and 176 half-sib progeny of cycle II. Additionally, to ensure the presence of the pathogen, we inoculated R. solanacearum 40–45 days after transplanting the seedlings. The incidence of the pathogen was assessed based on the number of plants showing symptoms (%). Although the difference between cycle 0 and cycle II was relatively small, the results suggest that recurrent selection was successful in accumulating resistance alleles, as reflected in the positive association between disease resistance and agronomic traits.
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Referências
Associação dos Fumicultores do Brasil. (2025). Safra de tabaco 2024/2025 fecha em 719.891 toneladas. https://afubra.com.br/safra-de-tabaco-2024-2025-fecha-em-719-891-toneladas/
Amaro, G. B., Abreu, Â. F. B., Ramalho, M. A. P., & Silva, F. B. (2007). Phenotypic recurrent selection in the common bean (Phaseolus vulgaris L.) with carioca-type grains for the resistance to the fungi Phaeoisariopsis griseola. Genetics and Molecular Biology, 30(3), 584-588. https://doi.org/10.1590/S1415-47572007000400014
Bernardo, R. (2020). Breeding for quantitative traits in plants. Stemma Press.
Chen, L., Guo, X., Xie, C., He, L., Cai, X., Tian, L., Song, B., & Liu, J. (2013). Nuclear and cytoplasmic genome components of Solanum tuberosum + S. chacoense somatic hybrids and three SSR alleles related to bacterial wilt resistance. Theoretical and Applied Genetics, 126(7), 1861-1872. https://doi.org/10.1007/s00122-013-2098-5.
Costa K. D. S., Santos, P. R., Santos, A. M. M., Silva, A. M. F., Chagas, J. T. B., Carvalho Filho, J. L. S., Pereira, J. W. L., Silva, M. O., Silva, J. R., & Menezes, D. (2019). Genetic control of tomato resistance to Ralstonia solanacearum. Euphytica, 215(7), 1-11. https://doi.org/10.1007/s10681-019-2458-6
Drake-Stowe, K., Bakaher, N., Goepfert, S., Philippon, B., Mark, R., Peterson, P., & Lewis, R. S. (2017). Multiple disease resistance loci affect soilborne disease resistance in tobacco (Nicotiana tabacum). Phytopathology, 107(9), 1055-1061. https://doi.org/10.1094/PHYTO-03-17-0118-R
Genin, S., & Denny, T. P. (2012). Pathogenomics of the Ralstonia solanacearum species complex. Annual Review of Phytopathology, 50, 67-89. https://doi.org/10.1146/annurev-phyto-081211-173000
Gonçalves, R. C., Vallim, J. H., Macedo, P. B. F., & Bastos, R. M. (2014). Método de inoculação de Ralstonia solanacearum para a seleção de plantas de Piper hispidinervum resistentes à murcha bacteriana. Embrapa. (Comunicado Técnico, 185).
Hallauer, A. R. (1992). Recurrent selection in maize. Plant Breeding Reviews, 9, 115-179.
Hayward, A.C. (1994) Systematic and phylogeny of Pseudomonas solanacearum and related bacteria. In A. C. Hayward, & G. L. Hartman (Eds.), Bacterial wilt: The disease and its causative agent, Pseudomonas solanacearum (pp. 123-155). CAB International.
Kakar, K. U., Nawaz, Z., Cui, Z., Ahemd, N., & Ren, X. (2020). Molecular breeding approaches for production of disease-resilient commercially important tobacco. Briefings in Functional Genomics, 19(1), 10-25. https://doi.org/10.1093/bfgp/elz038
Kannan, V. R., Bastas, K. K., & Devi, R. S. (2015). Sustainable approaches to controlling plant pathogenic bacteria In V. R. Kannan, & K. K Bastas (Eds.), Scientific and economic impact of plant pathogenic bacteria (pp. 369-392). CRC Press. https://doi.org/10.1201/b18892
Kim, B.-S., French, E., Caldwell, D., Harrington, E. J., & Iyer-Pascuzzi, A. S. (2016). Bacterial wilt disease: host resistance and pathogen virulence mechanisms. Physiological and Molecular Plant Pathology, 95, 37-43. https://doi.org/10.1016/j.pmpp.2016.02.007
Lan, T., Zhen, S., Yang, L., Wu, S., Wang, B., Zhang, S., Tong, Z., Chen, Y., Chen, S., Duan, Y., & Wu, W. (2014). Mapping of quantitative trait loci conferring resistance to bacterial wilt in tobacco (Nicotiana tabacum L.). Plant Breeding, 133(5), 672-677. https://doi.org/10.1111/pbr.12202
Lebeau, A., Gouy, M., Daunay, M. C., Wicker, E., Chiroleu, F., Prior, P., Frary, A., & Distinger, J. (2013). Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant. Theoretical and Applied Genetics, 126(1), 143-158. https://doi.org/10.1007/s00122-012-1969-5
Liu, X., Zhang, S., Jiang, Q., Bai, Y., Shen, G., Li, S., & Ding, W. (2016). Using community analysis to explore bacterial indicators for disease suppression of tobacco bacterial wilt. Scientific Reports, 6, 1-11. https://doi.org/10.1038/srep36773
Lopes, F. S., Leite, M. E., Porto, A. C. M., Miguel, L. A., Reis, V. O. L., & Santos, J. B. (2019). Recurrent selection in common bean aiming at resistance to white mold in a greenhouse. Crop Breeding and Applied Biotechnology, 19(1), 95-101. https://doi.org/10.1590/1984-70332019v19n1a13
Maimbo, M., Ohnishi, K., Hikichi, Y., Yoshioka, H., & Kiba, A. (2010). S-glycoprotein-like protein regulates defense responses in Nicotiana plants against Ralstonia solanacearum. Plant Physiology, 152(4), 2023-2035. https://doi.org/10.1104/pp.109.148189
Marques, T. L., Padua, J. M. V., Berger, I. J., & Ramalho, M. A. P. (2022). Strategies for the recurrent selection program in tobacco breeding for green leaf yield. Crop Science, 62(6), 2212-2221. https://doi.org/10.1002/csc2.20813
Miranda, E. F. O., Takatsu, A., & Uesugi, C. H. (2004). Colonização de raízes de plantas daninhas cultivadas in vitro e em vasos por Ralstonia solanacearum, biovares 1, 2 e 3. Fitopatologia Brasileira, 29(2), 121-127. https://doi.org/10.1590/S0100-41582004000200001
Morais Junior, O. P., Melo, P. G. S., Morais, O. P., Castro, A. P., Breseghello, F., Utumi, M. M., Pereira, J. A., Wruck, F. J., & Colombari Filho, J. M. (2015). Genetic progress after cycles of upland rice recurrent selection. Scientia Agricola, 72(4), 297-305. https://doi.org/10.1590/0103-9016-2014-0137
Nishi, T., Tajima, T., Noguchi, S., Ajisaka, H., & Negishi, H. (2003). Identification of DNA markers of tobacco linked to bacterial wilt resistance. Theoretical and Applied Genetics, 106, 765-770. https://doi.org/10.1007/s00122-002-1096-9
Qian, Y. L., Chen, J., Dong, J. J., Wu, Z. C., Liu, Y. H., Xue, B. Y., Shao, F. W., & Sun, X. Y. (2016). Genetic analyses of the major and minor locus groups of bacterial wilt resistance in tobacco using a diallel cross design. Genetics and Molecular Research, 15(1), 1-13. https://doi.org/10.4238/gmr.15017223
R Core Team. (2024). R: A language and environment for statistical computing. R Foundation for Statistical Computing. https://www.r-project.org/
Rezende, B. A., Abreu, A. F. B., Ramalho, M. A. P., & Souza, E. A. (2014). Severity evaluation methods in common bean recurrent selection programme for resistance to angular leaf spot. Journal of Phytopathology, 162(10), 643-649. https://doi.org/10.1111/jph.12238
Santiago, T. R., Lopes, C. A., Caetano‐Anollés, G., & Mizubuti, E. S. G. (2016). Phylotype and sequevar variability of Ralstonia solanacearum in Brazil, an ancient centre of diversity of the pathogen. Plant Pathology, 66(3), 383-392. https://doi.org/10.1111/ppa.12586
Tang, Q., Liu, T., Teng, K., Xiao, Z., Cai, H., Wang, Y., Xiao, Y., & Chen, W. (2023). Microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere. Frontiers in Plant Science, 14, 1-13. https://doi.org/10.3389/fpls.2023.1200136
Thiessen, L. (2020). Managing diseases. In L. R. Fisher (Ed.), Flue-cured tobacco guide (pp. 127-150). NC State Extension.
Viana, F. C., Berger, I. J., & Duarte, V. (2012). Caracterização de populações de Ralstonia solanacearum Smith em tabaco (Nicotiana tabacum L.) no Brasil. Tropical Plant Pathology, 37(2), 123-129. https://doi.org/10.1590/S1982-56762012000200004
Wang, J.-F., Ho, F.-I., Truong, H. T. H., Huang, S.-M., Balatero, C. H., Dittapongpitch, V., & Hidayati, N. (2013). Identification of major QTLs associated with stable resistance of tomato cultivar ‘Hawaii 7996’ to Ralstonia solanacearum. Euphytica, 190, 241-252. http://dx.doi.org/10.1007/s10681-012-0830-x
Wicker, E., Grassart, L., Coranson-Beaudu, R., Mian, D., Guibaud, C., Fegan, M., & Prior, P. (2007). Ralstonia solanacearum strains from Martinique (French West Indies) exhibiting a new pathogenic potential. Applied and Environmental Microbiology, 73(21), 6790-6801. https://doi.org/10.1128/AEM.00841-07
Yabuuchi, E., Kosako, Y., Yano, I., Hotta, H., & Nishiuchi, Y. (1995). Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. Nov. proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. Nov., Ralstonia solanacearum (Smith 1896) comb. Nov. and Ralstonia eutropha (Davis 1969) comb. Nov. Microbiology and Immunology, 39(11), 897-904. https://doi.org/10.1111/j.1348-0421.1995.tb03275.x
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Copyright (c) 2026 Reberth Renato da Silva, Gabriel da Silva Miguel, Fernanda Aparecida Castro Pereira, Magno Antonio Patto Ramalho, Ricardo Magela de Souza, José Maria Villela Pádua (Autor)
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