The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
Abstract
Agar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process.
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References
Berraquero, F. R., Baya, A. M., & Cormenzana, A. R. (1976). Establecimiento de indices para el estudio de la solubilizacion de fosfatos por bacterias del suelo. Ars Pharmceutica, 17(1), 399-406.
Ferreira, D. F. (2014). Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ciência e Agrotecnologia, 38(2), 109-112. DOI: 10.1590/S1413-70542014000200001
Ferreira, P. A. A., Bomfeti, C. A., Soares, B. L., & Moreira, F. M. S. (2012). Efficient nitrogen-fixing Rhizobium strains isolated from Amazonian soils are highly tolerant to acidity and aluminium. World Journal of Microbiology and Biotechnology, 28(5)1947-1959. DOI: 10.1007/s11274-011-0997-7
Fred, E. B., & Waksman, S. A. (1928). Laboratory manual of general microbiology – with special reference to the microorganism of the soil. New York, US: McGraw-Hill Book Company.
Hara, F. A. S., & Oliveira, L. A. (2005). Physiological and ecological characteristics of rhizobia isolates from acid soils of Iranduba, Amazonas. Pesquisa Agropecuária Brasileira, 40(7), 667-672. DOI: 10.1590/S0100-204X2005000700007
Liu, Z., Li, Y. C., Zhang, S., Fu, Y., Fan, X., Patel, J. S., & Zhang, M. (2015). Characterization of phosphate-solubilizing bacteria isolated from calcareous soils. Applied Soil Ecology, 96(1), 217-224. DOI: 10.1016/j.apsoil.2015.08.003
Marra, L. M, Oliveira, S. M., Soares, C. R. F. S., & Moreira, F. M. S. (2011). Solubilisation of inorganic phosphates by inoculant strains from tropical legumes. Scientia Agricola, 68(5), 603-609. DOI: 10.1590/S0103-90162011000500015
Marra, L. M, Soares, C. R. F. S., Oliveira, S. M., Ferreira, P. A. A., Soares, B. L., Carvalho, R. F., ... Lima, J. M., Moreira, F. M. S. (2012). Biological nitrogen fixation and phosphate solubilization by bacteria isolated from tropical soils. Plant and Soil, 357(1-2), 289-307. DOI: 10.1007/s11104-012-1157-z
Martins, C. E., Lima, W., Oliveira-Longatti, S. M., & Moreira, F. M. S. (2015). Phosphate-solubilising bacteria enhance Oryza sativa growth and nutrient accumulation in an oxisol fertilized with rock phosphate. Ecological Engineering, 83(1), 380-385. DOI: 10.1016/j.ecoleng.2015.06.045
Nautiyal, C. S. (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters, 170(1), 265-270. DOI: 10.1111/j.1574-6968.1999.tb13383.x
Oliveira-Longatti, S. M., Marra, L. M., Soares, B. L., Bomfetti, C. A., Silva, K., Ferreira, P. A. A., & Moreira, F. M. S. (2014). Bacteria isolated from soils of the western Amazon and from rehabilitated bauxite-mining areas have potential as plant growth promoters. World Journal of Microbiology and Biotechnology, 30(4), 1239-1250. DOI: 10.1007/s11274-013-1547-2
Peix, A., Rivas-Boyero, A. A., Mateos, P. F., Rodriguez-Barrueco, C., Martinez-Molina-E., & Velazquez, E. (2001). Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions. Soil Biology and Biochemistry, 33(1), 103-110. DOI: 10.1016/S0038-0717(00)00120-6
Rivas, R., Peix, A., Mateos, P. F., Trujillo, M. E., Martinez-Molina E., & Velazquez, E. (2006). Biodiversity of populations of phosphate solubilizing rhizobia that nodulates chickpea in different Spanish soils. Plant and Soil, 287(1-2), 23–33. DOI: 10.1007/s11104-006-9062-y
Sackett, W. G., Patten, A. J., & Brown, C. W. (1908). The solvent action of soil bacteria upon the insoluble phosphates of raw bone meal and natural raw rock phosphate. Zentralblatt fuer Bakteriologie: International Journal of Medical Microbiology, 28(1), 688-703.
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