Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS

Autores

  • Caio Cesar França Magnotti Universidade Federal de Santa Catarina http://orcid.org/0000-0002-1740-0540
  • Jorgélia de Jesus Pinto Castro Universidade de Santa Catarina
  • Fabiola Santiago Pedrotti Universidade de Santa Catarina
  • Fábio Carneiro Sterzelecki Universidade de Santa Catarina
  • Eduardo Gomes Sanches Instituto de Pesca
  • Vinicius Ronzani Cerqueira Universidade de Santa Catarina

DOI:

https://doi.org/10.4025/actascianimsci.v40i1.39563

Palavras-chave:

gamete conservation, spermatozoa, aquaculture, marine fish farming, near threatened fish.

Resumo

 

The present study aimed to evaluate the activation characteristics of the lebranche mullet spermatozoa in natura and diluted with CF-HBSS for 96h at 4±2°C. The semen was collected from eight wild fish in Florianópolis – SC (Brazil) (27°S) in May, during reproductive migration. Three pools of semen were divided into two treatments: in natura and diluted with CF-HBSS 1:3. The semen was activated with seawater (salinity of 34, pH 8.7 and 4±2°C) to determine: motility time, motility rate and sperm cell membrane integrity. Dilution with CF-HBSS 1:3 increased motility time of diluted semen (15-20% for 6, 12 and 18h). Motility rate was equal to fresh semen for up to 24h of refrigeration and it was 30% higher than in natura semen at 12, 18 and 24h. Cell membrane integrity was maintained in fresh semen up to 6h, and it was 15-20% higher than in natura semen from 6 to 48h (p < 0.05). The use of CF-HBSS as diluent in the ratio 1:3 is recommended for semen refrigeration of lebranche mullet.

 

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Biografia do Autor

  • Caio Cesar França Magnotti, Universidade Federal de Santa Catarina
    Trabalha no Departamento de Aquicultura da Universidade Federal de Santa Catarina. Alocado no Laboratório de Piscicultura Marinha em 2013, tem experiencia em cutivo multitrófico integrado (IMTA), sistemas de recirculação e reprodução de peixes marinhos. Fez doutorado em Aquicultura, na área de reprodução de peixes, com ênfase em análise e métodos de conservação de sêmen.

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Publicado

2018-04-01

Edição

Seção

Aquicultura

Como Citar

Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS. (2018). Acta Scientiarum. Animal Sciences, 40(1), e39563. https://doi.org/10.4025/actascianimsci.v40i1.39563