<b>Molecular characterization of ruminal bacterial diversity in vitro</b> - DOI: 10.4025/actascianimsci.v30i2.4699

  • Kárita Cláudia Freitas PUCPR
  • Jane Eyre Gabriel PUCPR
  • Laudí Cunha Leite USP
  • Rafael Dutra de Armas USP
  • Dante Pazzanese Duarte Lanna USP
  • Humberto Maciel França Madeira PUCPR
Palavras-chave: 16S rRNA, DGGE, rumen, microbial diversity.

Resumo

PCR analysis is a sensitive and specific tool to detect and monitor microorganisms in complex environmental samples. The amplification of 16S ribosomal DNA sequences followed by gel electrophoresis under denaturing gradient (DGGE) has been a powerful technique to genetically evaluate microbial ecosystems. Changes in rumen microbial populations were investigated in vitro using a basal diet with different lipid sources. PCRs were performed with two different sets of primers in order to amplify 16S rRNA sequences, and the amplified fragments were submitted to DGGE analysis. The findings presented in this study show that distinct microbial communities were present in each treatment. The presence of soybean oil seems to maximize growth of bacterial population, whereas fish oil appears to reduce growth. We demonstrated the successful application of molecular ecological techniques to analyze the structure and composition of bacterial communities in rumen ecosystems.

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Biografia do Autor

Humberto Maciel França Madeira, PUCPR
ATUALMENTE PARTICIPA DE 7 PROJETOS DE PESQUISA, SENDO QUE COORDENA 4 DESTES. ATUA NA AREA DE MICROBIOLOGIA, COM ENFASE EM MICROBIOLOGIA GASTRINTESTINAL. EM SUAS ATIVIDADES PROFISSIONAIS INTERAGIU COM 58 COLABORADORES EM CO-AUTORIAS DE TRABALHOS CIENTIFICOS. EM SEU CURRICULO LATTES OS TERMOS MAIS FREQUENTES NA CONTEXTUALIZACAO DA PRODUCAO CIENTIFICA, TECNOLOGICA E ARTISTICO-CULTURAL SAO: BIOINFORMATICA, BIOTECNOLOGIA, BIOLOGIA MOLECULAR, PRODUCAO ANIMAL, PROJETO GENOMA, ZOOTECNIA, BOVINOCULTURA DE CORTE, GENOMA, PREVOTELLA RUMINICOLA E NUTRICAO ANIMAL. Currículo Lattes
Publicado
2008-08-07
Como Citar
Freitas, K. C., Gabriel, J. E., Leite, L. C., Armas, R. D. de, Lanna, D. P. D., & Madeira, H. M. F. (2008). <b>Molecular characterization of ruminal bacterial diversity in vitro</b&gt; - DOI: 10.4025/actascianimsci.v30i2.4699. Acta Scientiarum. Animal Sciences, 30(2), 187-192. https://doi.org/10.4025/actascianimsci.v30i2.4699
Seção
Nutrição de Ruminantes

0.9
2019CiteScore
 
 
29th percentile
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