Histomorphological comparisons in testicles between Barb horse, donkey and mule
Resumo
The mule is a sterile hybrid domestic animal that results from the breeding of a male donkey with a female horse, understanding the reproductive biology of these species is very critical. The goal of this paper was to perform a comparative and more accurate histomorphometric of the testicles in Barb horse, donkeys and mules. Microscopic examinations and histological description were carried on genital tract of horses, donkeys and mules healthy and mature; this study was conducted during April-May 2018. The histological and the morphological results shows a similarity between the two equine species and the infertile hybrid for the testicles, the epididymis and the vas deferens. However, the difference was presented on the morphometric data; vas deferens was more voluminous in the horse and donkey than a mule. Moreover, the differences were significantly higher for the surface of the seminiferous tubules and for the epididymis. The lumen of the seminiferous tubules in mule was significantly higher than in the horse and donkey. Absence of gametes in the epididymal cavity and lower number of gametes in the mule. Furthermore, we have noted the presence of spermatozoa in one mule 16.67%. Therefore, the mule could complete development of spermatogenesis.
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Referências
Abou-Elhamd, A. S., Salem, A. O., & Selim, A. A. (2012). Histological and histochemical studies on the ampulla of the deferent duct of donkey (Equus asinus). Journal of Advanced Veterinary Research, 2(4), 261-270.
Canisso, I. F., Panzani, D., Miró, J., & Ellerbrock, R. E. (2019). Key, Aspects of Donkey and Mule Reproduction. The veterinary clinics of north Amrica Equine Practice, 35(3), 607-642. DOI: https://10.1016/j.cveq.2019.08.014.
Carluccio, A., Panzani, S., Contri, A., Branzo, V., Robbe, D., & Veronesi, M. C. (2013). Influence of season on testicular morphometry and semen characteristics in Martina Franca jackass. Theriogenology, 79(3), 502-507. DOI: https://10.1016/j.theriogenology.2012.11.005.
Chabchoub, A., Tibary, A., & Trimeche, A. (2007). Particularités et spécificités de la reproduction du baudet. Le nouveau praticien vétérinaire équine, 4(14), 36-40.
Chandley, A. C., Short, R. V., & Allen, W. R. (1975). Cytogenetic studies of three equine hybrids. Journal of Reproduction and Fertility Supplement, 23, 365-370.
Costa, G. M., Avelar, G. F., Rezende-Neto, J. V., Campos-Junior, P. H., Lacerda, S. M., Andrade, B. S., … França, L. R. (2012). Spermatogonial stem cell markers and niche in equids. PLoS One, 7(8), e44091.
Danmaigoro, A., Onu, J. E., Sonfada, M. L., Umaru, M. A., & Oyelowo, F. O. (2014). Histology and Histometric Anatomy of the Male Reproductive System of Bat (Eidolon helvum). Journal of Histology, 2014(4), 1-6. DOI: https://doi.org/10.1155/2014/834735.
Han, H., Wang, A., Liu, L., Zhao, G., Su, J., Wang, B., … Li, X. (2016). Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny. Asian-Australasian Journal of Animal Sciences, 29(6), 793-800. DOI: https://doi.org/10.5713/ajas.15.0670.
Hernandez, J. P., & Marquez, M. H. (1977). Fine strusture of mule testes: light and electron icroscopy study. American Journal of Veterinary Research, 38(4), 443-447.
Houssou, H., Bouzebda-Afri, F., Bouzebda, Z., Benidir, M., & Boujakji, A K. (2021). Measurement of the reproductive efficiency of Arabian stallions intended for AI in Algeria. Indian Journal of Animal Sciences. 91(7), 525-530.
Houssou, H., Bouzebda-Afri, F., Bouzebda, Z., & Haddouche, Z. (2018). A retrospective study of Arabian stallion fertility used in national stud farm of Tiaret (west of Algeria). Global Veterinaria, 20(3), 106-109. DOI: https://10.5829/idosi.gv. 2018.106.109.
Houssou, H., Bouzebda-Afri, F., Bouzebda, Z., & Benidir, M. (2020). Evaluation of sexual behavior of stallion (Arabian versus Barb) during breeding season in Algeria. Indian Journal of Animal Research, 54(9), 1078-1082. DOI: https://10.18805/ijar.B-950.
Iannuzzi, A., Pereira, J., Iannuzzi, C., Fu, B., & Ferguson-Smith, M. (2017). Pooling strategy and chromosome painting characterize a living zebroid for the first time. PLoS ONE, 12(7), e0180158. DOI: https://doi.org/10.1371/journal.pone.0180158.
Li, T., Wang, X., Zhang, H., Chen, Z., Zhao, X., & Ma, Y. (2019). Histomorphological Comparisons and Expression Patterns of BOLL Gene in Sheep Testes at Different Development Stages. Animals, 9(3), 105. DOI: https://10.3390/ani9030105.
McLean, A., Varnum, A., Ali, A., Heleski, C., & Navas González, F. J. (2019). Comparing and Contrasting Knowledge on Mules and Hinnies as a Tool to Comprehend Their Behavior and Improve Their Welfare. Animals, MDPI, 9(8), 488. DOI: https://doi.org/10.3390/ani9080488
Moustafa, M. N. K., Sayed, R., Zayed, A. E., & Abd El-Hafeez, H. H. (2015). Morphological and Morphometric Study of the Development of Seminiferous Epithelium of Donkey (Equus asinus) from Birth to Maturity. Journal of Cytology and Histology, 6(6), 1-8. DOI: https://10.4172/2157-7099.1000370.x.
Neves, E. M., Costa, G. M. J., & França, L. R. (2014). Sertoli cell and spermatogenic efficiencies in Pêga Donkey (Equus asinus). Animal Reproduction, 11(4), 517-525.
Neves, E. S., ChiarinI-Garacia, H., & Fança, L. R. (2002). Comparative testis morphometry and seminerous epithelium cycle length in donkeys and mules. Biology of Reproduction, 67(1), 247-255. DOI: https://10.1095/biolreprod67.1.247.
Pathak, V., Sheih, A., Rajput, R., Bherdwaj, R. L., & Sharma, A. (2013). Morphometric and histological observations on the testis of mule and donkey of himalayan region. Indian Journal of Veterinary Anatomy, 25(2), 99-101.
Pozor, M., Morrissey, H., Albanese, V., Khouzam, N., Deriberprey, A., Macpherson, M. L., & Kelleman, A. A. (2017). Relationship between echotextural and histomorphometric characteristics of stallion testes. Theriogenology, 99, 134-145. DOI: https://10.1016/j.theriogenology.2017.05.031.
Steiner C. C., & Ryder O. A. (2013). Characterization of Prdm9 in Equids and Sterility in Mules. PLoS ONE, 8(4), e61746. DOI: https://doi.org/10.1371/journal.pone.0061746.
Svingen, T., & Koopman, P. (2013). Building the mammalian testis: origins, differentiation, and assembly of the component cell populations. Genes & development, 27(22), 2409-2426. DOI: https://doi.org/10.1101/gad.228080.113.
Tripathi, U. K., Chhillar, S., Kumaresan, A., Aslam, M. K. M., Rajak, S. K., Nayak, S., … Yadav, S. (2015). Morphometric evaluation of seminiferous tubule and proportionate numerical analysis of Sertoli and spermatogenic cells indicate differences between crossbred and purebred bulls. Veterinary World, 8(5), 645-650. DOI: 10.14202/vetworld.2015.645-650.
Vilar, J. M., Batista, M., Carrillo, J. M., Rubio, M., Sopena, J., & Álamo, D. (2017). Histological, cytogenetic and endocrine evaluation in twenty-five unilateral cryptorchid horses. Journal of Applied Animal Research, 46(1), 441-444. DOI: https://10.1080/09712119.2017.1333435.
William, J., Bacha, J. R., & Bacha, L. M. (2012). Color atlas of veterinary histology: male reproductive system (3rd ed., p. 226). Oxford, UK: Wiley-Blackwell
Yang, T. A., Yang, Y. H., Song, X. C, Liu, L. L., Yang, Y. F., Xing, X. M., … Peng, Y. H. (2019). Comparative studies on testis, epididymis and serum hormone concentrations in foxes, and hybrids during the pre-breeding period. Animal Reproduction Science, 203, 61-67. DOI: https://10.1016/j.anireprosci.2019.02.008.
Zong, E. Z., & Fan, G. Q. (1989). The variety of sterility and gradual progression to fertility in hybrids of the horse and donkey. Heredity, 62, 393-406.
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