Next generation sequencing and bio-informatics analysis for the investigation of the vaginal bacterial microflora of clinically healthy mares

Antonia Mataragka; Andrianna Symeonidou; John Ikonomopoulos; Nikolaos Tzimotoudis; Georgia Diakoudi; Gianvito Lanave; Nicola Decaro; Eleni Papakonstantinou; Dimitrios Vlachakis

doi:10.4025/actascianimsci.v47i1.70939

Antonia Mataragka Agricultural University of Athens https://orcid.org/0000-0002-2539-4427
Andrianna Symeonidou Agricultural University of Athens
John Ikonomopoulos Agricultural University of Athens
Nikolaos Tzimotoudis Hellenic Army Biological Research Center
Georgia Diakoudi University of Bari Aldo Moro
Gianvito Lanave University of Bari Aldo Moro
Nicola Decaro University of Bari Aldo Moro
Eleni Papakonstantinou Agricultural University of Athens
Dimitrios Vlachakis Agricultural University of Athens / National and Kapodestrian University of Athens / Biomedical Research Foundation of the Academy of Athens / King’s College London

DOI: https://doi.org/10.4025/actascianimsci.v47i1.70939

Resumo

This study was focused on the investigation of the constitution of the vaginal bacterial microflora of mares, using 16Sr-RNA next generation sequencing and bio-informatics analysis. Samples were collected from the inner vaginal wall of 30 clinically healthy adult mares from various locations in central Greece. Successful reads were retrieved from 28 samples resulting to the presumptive identification of 192 bacterial species belonging to 87 genera. None of the bacterial genera or species that were detected was present in all the test samples, which indicates that the vaginal bacterial microflora of the study population is diverse. The bacterial genera detected the most in the test samples were Staphylococcus (89%) and Acinetobacter (54%). The bio-informatics analysis produced evidence of a much richer microbiota for the Greek native, compared to the Arabian and other breeds, and indicated variations in its constitution associated with the reproduction and vaccination records.

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