Investigating consequences of non-synonymous Single nucleotide polymorphisms of the Zyxin gene on protein structure and functions in Nigerian indigenous and Nera black chickens Zyxin

Adenaike Adeyemi  Sunday; Peters Sunday  Olusola; Fafiolu Adeboye  Olusesan; Waheed Abdullai  Adetunji; Abdulrahman Taofeek  Aireabakhame; Agaviezor Brilliant  Ogagaoghene; Agbalaya Khadijah  Kubura; Ikeobi Christian Obiora  Ndubuisi

doi:10.4025/actascianimsci.v47i1.71608

Adenaike Adeyemi Sunday Federal University of Agriculture / University of Port Harcourt https://orcid.org/0000-0002-9865-6029
Peters Sunday Olusola Berry College
Fafiolu Adeboye Olusesan , Federal University of Agriculture
Waheed Abdullai Adetunji Federal University of Agriculture
Abdulrahman Taofeek Aireabakhame Federal University of Agriculture
Agaviezor Brilliant Ogagaoghene University of Port Harcourt
Agbalaya Khadijah Kubura Lagos State University of Science and Technology
Ikeobi Christian Obiora Ndubuisi Federal University of Agriculture

DOI: https://doi.org/10.4025/actascianimsci.v47i1.71608

Palavras-chave: Zyxin; chicken coccidiosis; amino acid sequence; non-synonymous SNPs; naked neck chickens; single nucleotide polymorphisms (SNPs); nsSNPs.

Resumo

Zyxin functions as a regulator of the restructuring of the actin cytoskeleton during the process of repairing tissue damage, cell movement and attachment. It has also been identified as a potential gene involved in chicken coccidiosis. In order to gain a deeper understanding of these phenomena, we employed a collection of computer-based techniques and databases to examine the amino acid sequence, structural dynamics, molecular interactions, and activities of the gene. Our analysis revealed that Zyxin contains two non-synonymous SNPs (A > C at position 22 and G > A at position 137) at exon 1. Also, there existed a non-synonymous SNPs in Exon 3 (A>C and A>T both at position 861) of the gene with Synonymous SNPs observed only in exon 3 (A>G at position 812 and 854, T > C at position 863). The genetic diversity revealed in these chicken populations indicates the presence of genetic variation, with Naked neck chickens showing a considerably higher frequency of particular SNPs. Two non-synonymous single nucleotide polymorphisms (nsSNPs) were forecasted to exert a profound influence on the structure, stability, and activities of Zyxin, thereby heightening the vulnerability to coccidiosis.

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