Application of plant extracts as bio preservatives against Listeria monocytogenes and antioxidant in meat patties
Resumo
This study aimed to investigate the antioxidant and antibacterial properties of plant extracts derived from kiwi peels (Actinidia deliciosa), beet peels (Beta vulgaris L.), eggplant peels (Solanum melongena), and red cabbage leaves (Brassica oleracea L. var). Total phenols, flavonoids, free radical scavenging activity (DPPH), reducing power, and ferrous ion chelating capacity were evaluated. Beet peel extract showed the highest phenolic and flavonoid contents and exhibited the strongest DPPH scavenging activity, reaching 85% at 100 mg mL-1, as well as the most effective iron chelation, while eggplant peel showed the lowest activity. To assess preservative potential, kiwi and red cabbage extracts (0.5, 1, and 1.5%) were incorporated into minced meat patties inoculated with Listeria monocytogenes or stored as non-inoculated controls at 4 ± 1°C for 12 days. Microbiological analysis revealed significant reductions in total aerobic bacteria and L. monocytogenes counts, with kiwi peel extract showing the strongest inhibitory effect. Furthermore, treated patties demonstrated lower peroxide and free fatty acid values compared to controls (2.8 and 1.27 mEq kg-1, respectively, on day 12). These findings highlight the potential of selected plant extracts, particularly kiwi peels, as natural antioxidants and bio-preservatives in meat products.
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Referências
Aimen, F., & Preetham, E. (2024). Capability of AmLa extract on quality retardation of chilled stored Indian White Prawn (Fenneropenaeus Indicus Milne Edwards, 1837). International Journal of Fisheries and Aquaculture Research, 10(1), 1–12.
Al-Hamdani, S. A., & Al-Noor, J. M. (2024). The impact of glazing with natural preservatives on some qualitative characteristics of frozen-stored Japanese Threadfin Bream Nemipterus japonicas. Egyptian Journal of Aquatic Biology and Fisheries, 2, 327–341.
Andriyanov, P. A., Zhurilov, P. A., Liskova, E. A., Karpova, T. I., Sokolova, E. V., Yushina, Y. K., & Ermolaeva, S. A. (2021). Antimicrobial resistance of Listeria monocytogenes strains isolated from humans, animals, and food products in Russia in 1950–1980, 2000–2005, and 2018–2021. Antibiotics, 10(10), 1206.
Asadi, M., Ghasemnezhad, M., Olfati, J., Bakhshipour, A., Mirjalili, M. H., & Atak, A. (2024). Comparison of important quality components of red-flesh kiwifruit (Actinidia chinensis) in different locations. Open Agriculture, 9(1), 20220283.
Ashar, N., Ali, S., Asghar, B., Hussnain, F., Nasir, J., Nauman, K., & Badar, I. H. (2022). Application of ultrasound-assisted cooking temperature for improving physicochemical and sensory properties of broiler meat. Food Materials Research, 2(1), 1–6.
Banwo, K., Oduola, S., Alao, M., & Sanni, A. (2022). Hepatoprotective potentials of methanolic extracts of Roselle and beetroots against carbon tetrachloride and Escherichia coli induced stress in Wistar rats. Egyptian Journal of Basic and Applied Sciences, 9(1), 423–440.
Benyagoub, E., Kendoussi, K. F. Z., Mansour, K., Bouaicha, W., & Kaddouri, N. (2024). The in situ effect of Phoenician juniper leaves on the physicochemical and microbiological quality of fermented goat’s milk stored at room temperature. Al-Qadisiyah Journal For Agriculture Sciences, 14(1), 40–49.
Bonos, E., Skoufos, I., Petrotos, K., & Giavasis, I. (2022). Innovative use of olive, winery and cheese waste by-products as functional ingredients in broiler nutrition. Veterinary Sciences, 9(6). https://doi.org/10.3390/vetsci9060290
Brahma, P., & Baruah, S. (2023). Investigation of phytochemical constituents, GC-MS, DPPH free radical scavenging assay, and mineral contents of Glochidion sphaerogynum (Mull. Arg.) Kurz bark extract. Plant Science Today, 10(2), 98–105.
Cao, Y., Hao, R., Guo, Z., Han, L., Yu, Q., & Zhang, W. (2022). Combined effects of superchilling and natural extracts on beef preservation quality. Lebensmittel-Wissenschaft Technologie, 153, 112520.
Efenberger-Szmechtyk, M., Nowak, A., & Czyzowaka, A. (2021). Plant extracts rich in polyphenols: Antibacterial agents and natural preservatives for meat and meat products. Critical Reviews in Food Science and Nutrition, 61(1), 149–178.
El-Sawah, K. T., Mohamed El-Shahawy, R., Ibrahim. Nageeb, A., & Mohamed A, K. (2024). Antimicrobial activity of olive leaves extracts and application of leaves powder in meat preservation. Fayoum Journal of Agricultural Research and Development, 38(1), 45–55.
Fourati, M., Smaoui, S., Ben Hlima, H., Elhadef, K., Chakchouk Mtibaa, A., & Mellouli, L. (2020). Variability in phytochemical contents and biological potential of pomegranate (Punica granatum) peel extracts: Toward a new opportunity for minced beef meat preservation. Journal of Food Quality, 2020, 1–14.
Genstat. (2011). Genstat release 10.3DE. VSN International LTD.
Gyorgy, É., Laslo, É., & Csato, E. (2020). Antibacterial activity of plant extracts against isolated from ready-to-eat salads. Acta Universitatis Sapientiae, Alimentaria, 13(1), 131–143.
Hafidh, R. R., Abdulamir, A. S., Vern, L. S., Bakar, F. A., Abas, F., Jahanshiri, F., & Sekawi, Z. (2011). Inhibition of growth of highly resistant bacterial and fungal pathogens by a natural product. The open microbiology journal, 5, 96.
Hassan, F., Khan, A. U., Zaidi, S. Z. U. H., Niazi, M. K., & Ismail, M. A. (2023). In Vitro antioxidant and inhibitory study of Picrorhiza kurroa (Kutki), Syzygium aromaticum (Loung), Lawsonia inermis (Henna), Rheum emodi (Revand Chini), Curcuma longa (Haldi) against lipid per-oxidation in mice brain and liver. Dose-Response, 21(4), 15593258231210431.
Hu, Y. K., Kim, S. J., Jang, C. S., & Lim, S. D. (2024). Antioxidant activity analysis of native Actinidia arguta cultivars. International Journal of Molecular Sciences, 25(3), 1505.
Kamal, A. M., Taha, M. S., & Mousa, A. M. (2019). The radioprotective and anticancer effects of banana peels extract on male mice. J. Food Nutr. Res, 7(12), 827–835.
Karadeniz, M., Bakır, T. K., & Ünal, S. (2024). Investigation of the antioxidant and total phenolic substance of Fomes fomentarius and Ganoderma applanatum mushrooms showing therapeutic properties. Bilge International Journal of Science and Technology Research, 8(1), 14–18.
Kim, H. J., Sujiwo, J., Kim, H. J., & Jang, A. (2019). Effects of dipping chicken breast meat inoculated with Listeria monocytogenes in lyophilized scallion, garlic, and kiwi extracts on its physicochemical quality. Food Science of Animal Resources, 39(3), 418.
Liu, Y., Zhu, H., Dou, X., Jia, K., Panagou, E. Z., Zhang, H., & Dong, Q. (2024). The influence of nutrients on biofilm formation of an ST87 strain of Listeria monocytogenes. Lebensmittel-Wissenschaft Technologie, 191, 115658.
Loizzo, M. R., Tundis, R., Bonesi, M., Menichini, F., Mastellone, V., Avallone, L., & Menichini, F. (2012). Radical scavenging, antioxidant and metal chelating activities of Annona cherimola Mill.(cherimoya) peel and pulp in relation to their total phenolic and total flavonoid contents. Journal of Food Composition and Analysis, 25(2), 179–184.
Mikucka, W., Zielinska, M., Bulkowski, K., & Witonaka, I. (2022). Recovery of polyphenols from distillery stillage by microwave-assisted, ultrasound-assisted and conventional solid–liquid extraction. Scientific Reports, 12(1), 1–13. https://doi.org/10.1038/s41598-022-07322-0
Mogana, R., Adhikari, A., Tzar, M. N., RamLiza, R., & Wiart, C. (2020). Antibacterial activities of the extracts, fractions and isolated compounds from Canarium patentinervium Miq against bacterial clinical isolates. BMC complementary medicine and therapies, 20, 1–11.
Mohammad, A. J., & Andyousif, N. A. (2022). Molecular identification and assessment of bacterial contamination of frozen local and imported meat and chicken in Basrah, Iraq using 16S rDNA gene. Biodiversitas Journal of Biological Diversity, 23(3), 1598–1604.
Mokaizh, A. A. B., Nour, A. H., & Kerboua, K. (2024). Ultrasonic-assisted extraction to enhance the recovery of bioactive phenolic compounds from Commiphora gileadensis leaves. Ultrasonics Sonochemistry, 105, 106852.
Moura, A., Leclercq, A., Vales, G., Tessaud-Rita, N., Bracq-Dieye, H., Thouvenot, P., & Lecuiut, M. (2024). Phenotypic and genotypic antimicrobial resistance of Listeria monocytogenes: An observational study in France. The Lancet Regional Health–Europe, 37, 1–6.
Munekata, P. E. S., Rocchetti, G., Pateiro, M., Lucini, L., Dominguez, R., & Lorenzo, J. M. (2020). Addition of plant extracts to meat and meat products to extend shelf-life and health-promoting attributes: An overview. Current Opinion in Food Science, 31, 81–87.
Paiva, W. K. V., Medeiros, W. R. D. B., Assis, C. F., Dos Santos, E. S., & Sousa Junior, F. C. (2022). Physicochemical characterization and in vitro antioxidant activity of hyaluronic acid produced by Streptococcus zooepidemicus CCT 7546. Preparative Biochemistry and Biotechnology, 52(2), 234–243.
Patel, R. M. (2013). Ferrous ion chelating activity (FICA)-a comparative antioxidant activity evaluation of extracts of eleven naturally growing plants of Gujarat, India. International Journal of Scientific Research, 2(8), 426–428.
Reshma, P., & Suganthi, A. (2024). Determination of total phenol content and preliminary phytochemical analysis in Annona reticulata Linn. leaf extract. International Journal of Humanities and Sciences, 1(1), 30–34.
Saleh, S. O., Hussien, A. A., Youseef, A. G., Younis, W. K., & Mubarak, A. G. (2024). Prevalence, antibiotic resistance, and phylogenetic analysis of Listeria monocytogenes isolated from various sources in Egypt: Fish, vegetables, and humans. Iraqi Journal of Veterinary Sciences, 38(1), 15–27.
Sathisha, A. D., Lingaraju, H. B., & Prasad, K. S. (2011). Evaluation of antioxidant activity of medicinal plant extracts produced for commercial purpose. Journal of Chemistry, 8, 882–886.
Siddique, A., Idrees, N., Kashif, M., Ahmad, R., Ali, A., Siddiqua, A., & Javied, M. A. (2021). Antibacterial and antioxidant activity of Kiwi fruit. Biological and Clinical Sciences Research Journal, 2021(1), e028.
Sood, V., Tian, W., Narvaez‐Bravo, C., Amtfield, S. D., & Gonzalez, A. R. (2020). Plant extracts effectiveness to extend bison meat shelf life. Journal of Food Science, 85(4), 936–946.
The Association of Official Analysis Chemists. (1990). Official methods of analysis (15th ed.). The Association of Official Analysis Chemists.
Viera, V. B., Piovesan, N., Rodrigues, J. B., De O Mello, R., Prestes, R. C., Dos Santos. R. C. V., & Kubota, E. H. (2017). Extraction of phenolic compounds and evaluation of the antioxidant and antimicrobial capacity of red onion skin (Allium cepa L.). International Food Research Journal, 24(3), 990.
Wang, X. X., Chen, X., & Zhou, Z. K. (2024). Digestive and metabolic characteristics of dietary meat proteins: Meat source and thermal processing matter. Food Reviews International, 1–16.
Yu, L., Cheng, W., Tian, M., Wu, Z., Wei, X., Cheng, X., Yang, M., & Ma, X. (2024). Antioxidant activity and volatile oil analysis of ethanol extract of Phoebe zhennan S. Lee et FN Wei leaves. Forests, 15(2), 236.
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