Effects of feeding flaxseed to adult sheep on the fatty acid profile of freshly produced sausages
DOI:
https://doi.org/10.4025/actascianimsci.v48.i1.73676Palavras-chave:
animal nutrition; flaxseed; lipid profile; CLA; thermal stability; lipid oxidation.Resumo
Sheep meat, especially from adult animals, is high in saturated fatty acids due to extensive biohydrogenation of unsaturated fatty acids in their diet. This study explored how feeding sheep flaxseed could increase beneficial fatty acids in the meat and addresses a gap in the literature regarding the fatty acid profile of fresh sausages made from flaxseed-fed sheep meat. Fresh mixed sausage formulations were created with varying proportions of sheep and pork meat (100:0; 90:10; 80:20; 70:30, sheep:pork), with sheep fed a diet containing 15% flaxseed for 30, 45, and 60 days. The main fatty acids identified were oleic acid (C18:1 ω9c), palmitic acid (C16:0), and stearic acid (C18:0). The proportion of sheep meat did not significantly affect the fatty acid content of the sausages. However, sausages made with sheep meat from those fed flaxseeds for longer periods had higher levels of linolenic acid (C18:3 ω3) and conjugated linoleic acid (CLA, C18:2C9T11), along with a reduced ω6/ω3 ratio (4:1). TGA and DSC analyses showed lower stability and melting temperatures of fat from sausages with longer flaxseed feeding. Flaxseed inclusion in sheep diets resulted in a final product with higher nutritional value.
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Alamin, S. A. (2019). A study of sensory evaluation of sausages from beef and sheep meat. International Journal of Scientific Progress and Research, 162(62), 37-43.
Alencar, R. T., Vega, W. H. O., Silva, L. N. C. D. A., Costa, H. H. A., Parente, M. O. M., Lima, L. D. D. E., & Landim, A. V. (2022). Changes on the physicochemical and fatty acid profile of meat induced by inclusion of biscuit bran in lamb diet. Anais da Academia Brasileira de Ciências, 94(4), e20201365. https://doi.org/10.1590/0001-3765202220201365
Banskalieva, V., Sahlu, T., & Goetsch, A. L. (2000). Fatty acid composition of goat muscles and fat depots: a review. Small Ruminant Research, 37(3), 255-268. https://doi.org/10.1016/S0921-4488(00)00128-0
Bas, P., Berthelot, V., Pottier, E., & Normand, J. (2007). Effect of level of linseed on fatty acid composition of muscles and adipose tissues of lambs with emphasis on trans fatty acids. Meat Science, 77(4), 678-688. https://doi.org/10.1016/j.meatsci.2007.05.022
Berthelot, V., Bas, P., Pottier, E., & Normand, J. (2012). The effect of maternal linseed supplementation and/or lamb linseed supplementation on muscle and subcutaneous adipose tissue fatty acid composition of indoor lambs. Meat Science, 90(3), 548-557. https://doi.org/10.1016/j.meatsci.2011.09.014
Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911-917. https://doi.org/10.1139/o59-099
Bordoni, L., Zec, M., Naumovski, N., & Sergi, D. (2023). Editorial: The role of dietary fatty acids in metabolic health. Frontiers in Physiology, 14(1), 1-3. https://doi.org/10.3389/fphys.2023.1211151
De Tonnac, A., Karim-Luisset, S., & Mourot, J. (2017). Effect of different dietary linseed sources on fatty acid composition in pig tissues. Livestock Science, 203(1), 124-131. https://doi.org/10.1016/j.livsci.2017.03.022
Díaz, M. T., Cañeque, V., Sánchez, C. I., Lauzurica, S., Pérez, C., Fernández, C., Álvarez, I., & De la Fuente, J. (2011). Nutritional and sensory aspects of light lamb meat enriched in n−3 fatty acids during refrigerated storage. Food Chemistry, 124(1), 147-155. https://doi.org/10.1016/j.foodchem.2010.05.117
Doreau, M., Aurousseau, E., & Martin, C. (2009). Effects of linseed lipids fed as rolled seeds, extruded seeds or oil on organic matter and crude protein digestion in cows. Animal Feed Science and Technology, 150(3), 187-196. https://doi.org/10.1016/j.anifeedsci.2008.09.004
Fu, Y., Cao, S., Yang, L., & Li, Z. (2022). Flavor formation based on lipid in meat and meat products: a review. Journal of Food Biochemistry, 46(12), e14439. https://doi.org/10.1111/jfbc.14439
Hartman, L., & Lago, R. C. (1973). Rapid preparation of fatty acid methyl esters from lipids. Laboratory Practice, 22(6), 475-476.
Iswoyo, Sumarmono, J., & Setyawardani, T. (2023). Physical characteristics of emulsion-type sausage from lamb meat with varying fat levels. IOP Conference Series: Earth and Environmental Science, 1177(1), 12034. https://doi.org/10.1088/1755-1315/1177/1/012034
Jiang, J., Tang, X., Xue, Y., Lin, G., & Xiong, Y. L. (2017). Dietary linseed oil supplemented with organic selenium improved the fatty acid nutritional profile, muscular selenium deposition, water retention, and tenderness of fresh pork. Meat Science, 131(1), 99-106. https://doi.org/10.1016/j.meatsci.2017.03.014
Kouba, M., & Mourot, J. (2011). A review of nutritional effects on fat composition of animal products with special emphasis on n-3 polyunsaturated fatty acids. Biochimie, 93(1), 13-17. https://doi.org/10.1016/j.biochi.2010.02.027
Kris-Etherton, P. M., & Krauss, R. M. (2020). Public health guidelines should recommend reducing saturated fat consumption as much as possible: YES. The American Journal of Clinical Nutrition, 112(1), 13-18. https://doi.org/10.1093/ajcn/nqaa110
Lee, S. P. S., Dart, A. M., Walker, K. Z., O’Dea, K., Chin-Dusting, J. P. F., & Skilton, M. R. (2012). Effect of altering dietary n-6:n-3 PUFA ratio on cardiovascular risk measures in patients treated with statins: a pilot study. British Journal of Nutrition, 108(7), 1280-1285. https://doi.org/10.1017/S0007114511006519
Lima, T. L. S., da Costa, G. F., da Silva Araújo, Í. B., da Cruz, G. R. B., Ribeiro, N. L., Filho, E. M. B., Domínguez, R., & Lorenzo, J. M. (2021). Pre-emulsioned linseed oil as animal fat replacement in sheep meat sausages: Microstructure and physicochemical properties. Journal of Food Processing and Preservation, 45(1), e15051. https://doi.org/10.1111/jfpp.15051
Mukhametov, A., Yerbulekova, M., Aitkhozhayeva, G., Tuyakova, G., & Dautkanova, D. (2022). Effects of ω-3 fatty acids and ratio of ω-3/ω-6 for health promotion and disease prevention. Food Science and Technology, 42(1), e58321. https://doi.org/10.1590/fst.58321
Peng, Y. S., Brown, M. A., Wu, J. P., & Liu, Z. (2010). Different oilseed supplements alter fatty acid composition of different adipose tissues of adult ewes. Meat Science, 85(3), 542-549. https://doi.org/10.1016/j.meatsci.2010.03.003
Raharjo, S., Sofos, J. N., & Schmidt, G. R. (1992). Improved speed, specificity, and limit of determination of an aqueous acid extraction thiobarbituric acid-C18 method for measuring lipid peroxidation in beef. Journal of Agricultural and Food Chemistry, 40(11), 2182-2185. https://doi.org/10.1021/jf00023a027
Realini, C. E., Bianchi, G., Bentancur, O., & Garibotto, G. (2017). Effect of supplementation with linseed or a blend of aromatic spices and time on feed on fatty acid composition, meat quality and consumer liking of meat from lambs fed dehydrated alfalfa or corn. Meat Science, 127(1), 21-29. https://doi.org/10.1016/j.meatsci.2016.12.013
Russo, C., Preziuso, G., Casarosa, L., Campodoni, G., & Cianci, D. (1999). Effect of diet energy source on the chemical–physical characteristics of meat and depot fat of lambs carcasses. Small Ruminant Research, 33(1), 77-85. https://doi.org/10.1016/S0921-4488(98)00200-4
Sathivel, S., Prinyawiwatkul, W., Negulescu, I. I., & King, J. M. (2008). Determination of melting points, specific heat capacity and enthalpy of catfish visceral oil during the purification process. Journal of the American Oil Chemists’ Society, 85(3), 291-296. https://doi.org/10.1007/s11746-007-1191-9
Seifdavati, J., & Taghizadeh, A. (2023). Fatty acid composition of meat from lambs fed diets containing moist-heat treated legume grains. African Journal of Food Science Research, 11(1), 1-8. https://doi.org/10.46882/AJFSR/1153
Souza, H. B. A., Silva-Sobrinho, A. G., Zeola, N. M. B. L., Pelicano, E. R. L., Souza, P. A., Leonel, F. R., Oba, A., & Lima, T. M. A. (2005). Avaliação de diferentes teores de gordura e antioxidante natural nos parametros qualitativos e sensoriais de embutido fresco de carne ovina. ARS Veterinaria, 21(3), 315-319.
Statsoft, Inc. (1995). Statistica (data analysis software system), version 5.0. StatSoft, Inc.
Teixeira, A., Silva, S., Guedes, C., & Rodrigues, S. (2020). Sheep and goat meat processed products quality: a review. Foods, 9(7), 960. https://doi.org/10.3390/foods9070960
Tibaoui, S., Smeti, S., Essid, I., Bertolín, J. R., Joy, M., & Atti, N. (2020). Physicochemical characteristics, fatty acid profile, alpha-tocopherol content, and lipid oxidation of meat from ewes fed different levels of distilled myrtle residues. Molecules, 25(21), 4975. https://doi.org/10.3390/molecules25214975
Wachira, A. M., Sinclair, L. A., Wilkinson, R. G., Enser, M., Wood, J. D., & Fisher, A. V. (2002). Effects of dietary fat source and breed on the carcass composition, n-3 polyunsaturated fatty acid and conjugated linoleic acid content of sheep meat and adipose tissue. British Journal of Nutrition, 88(6), 697-709. https://doi.org/10.1079/BJN2002727
Wang, B., Pace, R. D., Dessai, A. P., Bovell-Benjamin, A., & Phillips, B. (2002). Modified extraction method for determining 2-thiobarbituric acid values in meat with increased specificity and simplicity. Journal of Food Science, 67(8), 2833-2836. https://doi.org/10.1111/j.1365-2621.2002.tb08824.x
Wood, J. D., Richardson, R. I., Nute, G. R., Fisher, A. V, Campo, M. M., Kasapidou, E., Sheard, P. R., & Enser, M. (2004). Effects of fatty acids on meat quality: a review. Meat Science, 66(1), 21-32. https://doi.org/10.1016/S0309-1740(03)00022-6
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Copyright (c) 2026 Andresa Carla Feihrmann , Bruno Fischer, Lisiane dos Santos Freitas , Francisco de Assis Fonseca de Macedo , Alexander Junges, Rogério Luis Cansian, Eunice Valduga

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