<b>Production and partial characterization of proteases from <i>Mucor hiemalis</i> URM3773

  • Roana Cecília dos Santos Ribeiro Unidade Acadêmica de Garanhuns - Universidade Federal Rural de Pernambuco
  • Thaís Rafaelle dos Santos Ribeiro Universidade Federal de Campina Grande
  • Cristina Maria de Souza-Motta Universidade Federal de Pernambuco
  • Erika Valente Medeiros Universidade Federal Rural de Pernambuco
  • Keila Aparecida Moreira Universidade Federal Rural de Pernambuco

Resumo

The current study evaluated the proteases production from 11 fungal species belonging to the genera Mucor, Rhizomucor and Absidia. The species were obtained from the Collection of Cultures URM at the Mycology Department-UFPE, Brazil. The best producing species was Mucor hiemalis URM 3773 (1.689 U mL-1). Plackett-Burman design methodology was employed to select the most effective parameter for protease production out of 11 medium components, including: concentration of filtrate soybean, glucose, incubation period, yeast extract, tryptone, pH, aeration, rotation, NH4Cl, MgSO4 and K2HPO4. Filtrated soybean concentration was the significant variable over the response variable, which was the specific protease activity. The crude enzyme extract showed optimal activity in pH 7.5 and at 50ºC. The enzyme was stable within a wide pH range from 5.8 to 8.0, in the phosphate buffer 0.1M and in stable temperature variation of 40-70ºC, for 180 minutes. The ions FeSO4, NaCl, MnCl2, MgCl2 and KCl stimulated the protease activity, whereas ZnCl2 ion inhibited the activity in 2.27%. Iodoacetic acid at 1mM was the proteases inhibitor that presented greater action.The results indicate that the studied enzyme have great potential for industrial application.

 

 

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Publicado
2015-03-10
Como Citar
Ribeiro, R. C. dos S., Ribeiro, T. R. dos S., Souza-Motta, C. M. de, Medeiros, E. V., & Moreira, K. A. (2015). <b>Production and partial characterization of proteases from <i>Mucor hiemalis</i&gt; URM3773. Acta Scientiarum. Biological Sciences, 37(1), 71-79. https://doi.org/10.4025/actascibiolsci.v37i1.21016
Seção
Microbiologia

 

0.6
2019CiteScore
 
 
31st percentile
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0.6
2019CiteScore
 
 
31st percentile
Powered by  Scopus