<b>The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs

  • Emanuela Garbin Martinazzo Universidade Federal de Pelotas; Universidade Federal do Rio Grande
  • Anelise Tessari Perbini Universidade Federal de Pelotas; Universidade Federal do Oeste do Pará
  • Marcos Antonio Bacarin Universidade Federal de Pelotas

Resumo

The mechanisms of photosynthetic electron transport can be elucidated by inhibition of electron flow through the use of specific substances that, when combined with the chlorophyll  chlorophyll a fluorescence emission was measured to investigate the effect of several inhibitors of the photosynthetic electron transport chain in canola leaf discs. Leaf discs were incubated in the dark for 2 hours in different solutions: (a) water – control; (b) DCMU at 500 µM; (c) phenanthroline at 10 mM; and (d) hydroxylamine at 10, 50, 100 and 200 mM. Similar effects were observed between DCMU and phenanthroline, the initial fluorescence value was altered, but not the maximum fluorescence, with the disappearance of the IP phase. Hydroxylamine interacted and inhibited the oxygen evolving complex and caused an imbalance between the rate of QA reduction by photosystem II and the rate of QA oxidation by photosystem I.

 

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Publicado
2015-08-06
Como Citar
Martinazzo, E. G., Perbini, A. T., & Bacarin, M. A. (2015). <b&gt;The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs. Acta Scientiarum. Biological Sciences, 37(2), 159-167. https://doi.org/10.4025/actascibiolsci.v37i2.23263
Seção
Biotecnologia

 

0.6
2019CiteScore
 
 
31st percentile
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0.6
2019CiteScore
 
 
31st percentile
Powered by  Scopus