<b>PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography

  • Moacyr Jesus Barreto de Melo Rêgo Universidade Federal de Pernambuco
  • Luiza Rayanna Amorim de Lima Universidade Federal de Pernambuco
  • Andrei Figueiredo Passo Longo Universidade Federal de Pernambuco
  • Gilson Alves Nascimento-Filho Universidade Federal de Pernambuco
  • Luiz Bezera de Carvalho Júnior Universidade Federal de Pernambuco
  • Eduardo Isidoro Carneiro Beltrão Universidade Federal de Pernambuco

Resumo

Immobilized lectins are a powerful biotechnological tool for separation and isolation of glycoconjugates. In the present study, polyvinyl alcohol (PVA) and glutaraldehyde (GA) were used as a support for Concanavalin A (Con A) covalent immobilization and for entrapment of Parkia pendula seed gum (PpeG). Con A immobilization yielded approximately 30% and 0.6 M glucose solution was the minimum concentration able to elute fetuin from column. PVA-GA-PpeG column was efficiently recognized by pure P. pendula lectin (PpeL). These findings indicate that PVA-GA interpenetrated network showed to be an efficient support for lectin covalent immobilization and as affinity chromatography matrix after trapping of PpeG.

 

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Publicado
2016-12-08
Como Citar
Rêgo, M. J. B. de M., Lima, L. R. A. de, Longo, A. F. P., Nascimento-Filho, G. A., Carvalho Júnior, L. B. de, & Beltrão, E. I. C. (2016). <b&gt;PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography. Acta Scientiarum. Biological Sciences, 38(3), 291-295. https://doi.org/10.4025/actascibiolsci.v38i3.31369
Seção
Biotecnologia

 

0.6
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0.6
2019CiteScore
 
 
31st percentile
Powered by  Scopus