Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571

Autores

  • Luana Paula de Azevedo de Oliveira Universidade Federal do Recôncavo da Bahia Autor
  • Tiago Felipe Oliveira Universidade Federal do Recôncavo da Bahia Autor
  • Jonas Contiero Universidade Estadual Paulista "Julio de Mesquita Filho" Autor
  • Márcia Luciana Cazetta Universidade Federal do Recôncavo da Bahia Autor

DOI:

https://doi.org/10.4025/actascibiolsci.v38i3.31489

Palavras-chave:

yeast, microbial enzymes, agricultural substrate

Resumo

The factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours.

 

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Publicado

2016-12-08

Edição

Seção

Biotecnologia

Como Citar

Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571. (2016). Acta Scientiarum. Biological Sciences, 38(3), 283-289. https://doi.org/10.4025/actascibiolsci.v38i3.31489

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