<b>Isolation of <i>Saprolegnia aenigmatica</i> oomycetes and protocol for experimental infection of pacu (<i>Piaractus mesopotamicus</i>)

  • Silvia Patrícia Carraschi Universidade de Araraquara http://orcid.org/0000-0002-5170-8901
  • Nathália Garlich Universidade Estadual Paulista
  • Andressa Souza-Pollo Universidade Estadual Paulista
  • Daniela Isabel Brayer Pereira Universidade Federal de Pelotas
  • Claudinei da Cruz Centro Universitário da Fundação Educacional de Barretos
  • Maria José Tavares Ranzani-Paiva Instituto de Pesca

Resumo

 

Successful disease treatment depends on molecular studies under indoor conditions with experimental infection protocols that facilitate understanding the disease and the drug`s efficacy. The internal transcribed spacer (ITS) region was sequenced from three isolates, which were identified as Saprolegnia aenigmatica. Subsequently, healthy fish were immunosuppressed with dexamethasone (1.2 mg kg-1) and descaled to the skin using a sharp scalpel. These individuals were isolated in individual aquariums maintained at 22°C. Individuals in one group were subcutaneously inoculated with 9,000 zoospores (DDZ treatment), a second group was exposed to oomycetes in water with three colonized baits (DDB), a third group was maintained in water without zoospores (DD), and a control group (C) consisted of healthy animals. After 48 and 96 hours, two animals from each group were euthanized for fungal re-isolation. The fish from groups DD and C did not show clinical signs, and no oomycetes were isolated. The animals from the DDZ and DDB groups showed cotton-wool-like masses on the skin, and S. aenigmatica was re-isolated. Thus, for infection using zoospores or baits parasitized by S. aenigmatica, an immunosuppressor (dexamethasone) and a sharp scalpel can be used effectively to establish an experimental infection in P. mesopotamicus.

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Publicado
2018-04-01
Como Citar
Carraschi, S. P., Garlich, N., Souza-Pollo, A., Pereira, D. I. B., Cruz, C. da, & Ranzani-Paiva, M. J. T. (2018). <b>Isolation of <i>Saprolegnia aenigmatica</i> oomycetes and protocol for experimental infection of pacu (<i>Piaractus mesopotamicus</i&gt;). Acta Scientiarum. Biological Sciences, 40(1), e38186. https://doi.org/10.4025/actascibiolsci.v40i1.38186
Seção
Zoologia

 

0.6
2019CiteScore
 
 
31st percentile
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0.6
2019CiteScore
 
 
31st percentile
Powered by  Scopus