Effect of heat treatment and osmotic storage on the control of fungi associated with short-lived Inga vera Willd. Embryos

  • Rayana Martins Instituto de Pesquisas Ambientais, Núcleo de Conservação da Biodiversidade https://orcid.org/0000-0002-6105-9001
  • Claudio José Barbedo Instituto de Pesquisas Ambientais, Núcleo de Conservação da Biodiversidade
  • Nelson Augustos dos Santos Junior Instituto de Pesquisas Ambientais, Núcleo de Conservação da Biodiversidade
Palavras-chave: fungi incidence; osmotherapy in seeds; recalcitrant seeds; thermotherapy in seeds.

Resumo

Seeds sensitive to desiccation remain metabolically active after dispersion with a high water content, which makes their storage for long periods difficult, due to their deterioration and high proliferation of fungi. One of the ways to maintain or improve the sanitary quality of the seed, preventing the spread and transmission of fungi, is the treatment of these seeds and how they are stored. Thus, Inga vera seeds were subjected to thermotherapy in hot water at different temperatures and submersion periods and to storage in an osmotic polyethylene glycol solution (-2.0 MPa) for 25, 50 and 75 days. They were then evaluated for health (incidence and severity). It was found that osmotic storage was more efficient than heat treatment in reducing the majority of fungi found. The association of both types of treatment produces favorable results and the reapplication of heat treatment can increase fungal control throughout storage. The severity assessment made it possible to detect differences between treatments that were not identifiable in the incidence assessment.

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Referências

Andréo, Y., Nakagawa, J., & Barbedo, C. J. (2006). Mobilização de água e conservação da viabilidade de embriões de sementes recalcitrantes de ingá (Inga vera Willd. subsp. affinis (DC.) TD Pennington). Brazilian Journal of Botany, 29(2), 309-318. https://doi.org/10.1590/S0100-84042006000200012

Bilia, D. A. C., & Barbedo, C. J. (1997). Estudos de germinação e armazenamento de sementes de Inga uruguensis Hook. et Arn. Científica, 25(2), 379-391.

Bonjovani, M. R., & Barbedo, C. J. (2008). Sementes recalcitrantes: intolerantes a baixas temperaturas? Embriões recalcitrantes de Inga vera Willd. subsp. affinis (DC.) TD Penn. toleram temperatura sub-zero. Brazilian Journal of Botany, 31(2), 345-356. https://doi.org/10.1590/S0100-84042008000200017

Bonjovani, M. R., & Barbedo, C. J. (2014). Induction of tolerance to desiccation and to subzero temperatures in embryos of recalcitrant seeds of inga. Journal of Seed Science, 36(4), 419-426. https://doi.org/10.1590/2317-1545v36n41027

Brasil. (2009a). Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Secretaria de Defesa Agropecuária. MAPA/ACS.

Brasil. (2009b). Ministério da Agricultura, Pecuária e Abastecimento. Manual de Análise Sanitária de Sementes. Secretaria de Defesa Agropecuária. MAPA/ACS.

Brasil. (2020). Ministério da Agricultura, Pecuária e Abastecimento. Coordenação Geral de Agrotóxicos e Afins/DFIA/SDA. http://agrofit.agricultura.gov.br/agrofit_cons/principal _agrofit_cons

Calistru, C., McLean, M., Pammenter, N. W., & Berjak, P. (2000). The effects of mycofloral infection on the viability and ultrastructure of wet-stored recalcitrant seeds of Avicennia marina (Forssk.) Vierh. Seed Science Research, 10(3), 341-353. https://doi.org/10.1017/S0960258500000386

Christensen, C. M., & Kaufmann, H. H. (1965). Deterioration of stored grains by fungi. Annual Review of Phytopathology, 3, 69-84. https://doi.org/10.1146/annurev.py.03.090165.000441

Colville, L., & Kranner, I. (2010). Desiccation tolerant plants as model systems to study redox regulation of protein thiols. Plant Growth Regulation, 62, 241-255. https://doi.org/10.1007/s10725-010-9482-9

Delgado, L. F., & Barbedo, C. J. (2007). Tolerância à dessecação de sementes de espécies de Eugenia. Pesquisa Agropecuária Brasileira, 42(2), 265-272. https://doi.org/10.1590/S0100-204X2007000200016

Delgado, L. F., & Barbedo, C. J. (2012). Water potential and viability of seeds of Eugenia (Myrtaceae), a tropical tree species, based upon different levels of drying. Brazilian Archives of Biology and Technology, 55(4), 583-590. https://doi.org/10.1590/S1516-89132012000400014

Faria, J. M. R., van Lammeren, A. A. M., & Hilhorst, H. W. M. (2004). Desiccation sensitivity and cell cycle aspects in seeds of Inga vera subsp. affinis. Seed Science Research, 14(2), 165-178. https://doi.org/10.1079/SSR2004166

Françoso, C. F., & Barbedo, C. J. (2014). Tratamentos osmóticos e térmicos para controle de fungos em sementes de grumixameira (Eugenia brasiliensis Lam.) e pitangueira (Eugenia uniflora L.). Hoehnea, 41(4), 541-552. https://doi.org/10.1590/2236-8906-30/2013

Françoso, C. F., & Barbedo, C. J. (2016). Osmotic and heat treatments on control of fungi associated with seeds of Eugenia brasiliensis and E. pyriformis (Myrtaceae). Journal of Seed Science, 38(3), 195-203. https://doi.org/10.1590/2317-1545v38n3159481

Garcia, D. C., Barros, A. C. S. A., Peske, S. T., & Menezes, N. L. (2004). A secagem de sementes. Ciência Rural, 34(2), 603-608. https://doi.org/10.1590/S0103-84782004000200045

Hong, T. D., & Ellis, R. H. (1996). A protocol to determine seed storage behaviour. International Plant Genetic Resources Institute. (IPGRI Technical Bulletin No. 1).

Kohama, S., Maluf, A. M., Bilia, D. A. C., & Barbedo, C. J. (2006). Drying and storage of seeds Eugenia brasiliensis Lam. (Grumixameira). Journal of Seed Science, 28(1), 72-78. https://doi.org/10.1590/S0101-31222006000100010

Machado, J. D. C. (2000). Tratamento de sementes no controle de doenças. LAPS/UFLA/FAEPE Press.

Marcos Filho, J. (2015). Fisiologia de sementes de plantas cultivadas. ABRATES Press.

Mendes, M. A. S., Lima, P. M. M. P., Fonseca, J. N. L., & Santos, M. F. (2001). Erradicação de Fusarium oxysporum em sementes de alfafa utilizando termo e quimioterapia. Tropical Plant Pathology, 26(2), 148-152. https://doi.org/10.1590/S0100-41582001000200005

Michel, B. E., & Kaufmann, M. R. (1973). The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51(5), 914-916. https://doi.org/10.1104/pp.51.5.914

Mukherjee, M., Watt, D. A., & Berjak, P. (2006). Molecular detection and diagnosis of fungal contaminants of recalcitrant seeds: Quercus robur L. acorns as a model system. Seed Science and Technology, 34(2), 415-427. https://doi.org/10.15258/sst.2006.34.2.17

Mycock, D. J., & Berjak, P. (1990). Fungal contaminants associated with several homoiohydrous (recalcitrant) seed species. Phytophylactica, 22(4), 413-418.

Oliveira, C. F. D., Oliveira, D. C., Parisi, J. J. D., & Barbedo, C. J. (2011). Deterioração de sementes de espécies brasileiras de Eugenia em função da incidência e do controle de fungos. Revista Brasileira de Sementes, 33(3), 520-532. https://doi.org/10.1590/S0101-31222011000300015

Parisi, J. J. D., Biagi, J. D., Barbedo, C. J., & Medina, P. F. (2013). Viability of Inga vera Willd. subsp. affinis (DC.) TD Penn. embryos according to the maturation stage, fungal incidence, chemical treatment and storage. Journal of Seed Science, 35(1), 70-76. https://doi.org/10.1590/S2317-15372013000100010

Parisi, J. J. D., Biagi, J. D., Medina, P. F., & Barbedo, C. J. (2016). Fungicide and drying effects on the viability of recalcitrant seeds of Inga vera subsp. affinis. Tropical Plant Pathology, 41(3), 177-182. https://doi.org/10.1007/s40858-016-0084-6

Parisi, J. J. D., Biagi, J. D., Barbedo, C. J., Medina, P. F., & Lamarca, E. V. (2019). Respiratory rates of Inga vera Willd. subsp. affinis (DC.) TD Penn. seeds. Floresta e Ambiente, 26(4), 1-10. https://doi.org/10.1590/2179-8087.103317

Roberts, E. H. (1973). Predicting the storage life of seeds. Seed Science and Technology, 1(3), 499-514.

Silva, E. C. C., Rufini, J. C. M., Parrella, N. N. L. D., & Neves, W. S. (2018). Qualidade fisiológica e fitossanitária de sementes tratadas de Eugenia dysenterica DC. durante armazenamento. Revista Agrária Acadêmica, 1(3), 65-75. https://doi.org/10.32406/v1n32018/65-75agrariacad

Systat software. (2011). SigmaPlot for Windows Version 12.0. Systat Software Inc.

Vertucci, C. W., & Farrant, J. M. (1995). Acquisition and loss of desiccation tolerance. In J Kigel, & G Galili (Ed.), Seed development and germination (pp. 237-271). CRC Press.

Vieira, J. F., Abreu, M. S., Maia, F. G. M., Ogoshi, C., Pierre, R. O., Carvalho, E. A., & Silva, B. M. (2011). Tratamento térmico de sementes no manejo da mancha manteigosa causada por Colletotrichum gloeosporioides em cafeeiro (Coffea arabica L.). Acta Ambiental Catarinense, 8(1/2), 30-38. https://doi.org/10.24021/raac.v8i1/2.1927

Publicado
2025-08-08
Como Citar
Martins, R., Barbedo, C. J., & Santos Junior, N. A. dos. (2025). Effect of heat treatment and osmotic storage on the control of fungi associated with short-lived Inga vera Willd. Embryos . Acta Scientiarum. Biological Sciences, 47(1), e73383. https://doi.org/10.4025/actascibiolsci.v47i1.73383
Seção
Botânica

Funding data

 

0.6
2019CiteScore
 
 
31st percentile
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0.6
2019CiteScore
 
 
31st percentile
Powered by  Scopus