Bioactive compounds, anti-oxidant and anti-inflammatory effects of carob fruit and leaves (Ceratonia siliqua)
Resumo
The present study aimed to make a phytochemical study and to evaluate the in vitro antioxidant and anti-inflammatory activities of the crude extracts of two parts (leaves and fruit) of Ceratonia siliqua. The polyphenols extraction step was followed by the determination of polyphenols and flavonoids contents. The antioxidant activity was evaluated using the DPPH test and the ferric reducing power. Protein denaturation (BSA) and red blood cell membrane stabilization assays were used for the anti-inflammatory activity. Phytochemical assays showed that leaves extract was richer in polyphenols and flavonoids compared to fruit. The DPPH antiradical activity and the ferric reducing power revealed that the methanol extract of the leaves has the highest antioxidant activity compared to the fruit. For the anti-inflammatory activity, the results also showed that the crude extract of the leaves has a powerful activity against denaturation of proteins (BSA), and has an important protective effect against membrane hemolysis of red blood cells compared to fruit extract. The obtained results suggested that Ceratonia siliqua is an effective antioxidant and anti-inflammatory agent.
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Abidar, S., Boiangiu, R. S., Dumitru, G., Todirascu-Ciornea, E., Amakran, A., Cioanca, O., Hritcu, L., & Nhiri, M. (2020). The aqueous extract from Ceratonia siliqua leaves protects against 6-hydroxydopamine in zebrafish: understanding the underlying mechanism. Antioxidants, 9(4), 1-21. https://doi.org/10.3390/antiox9040304
Alabdallat, N. G., & Bilto, Y. (2015). Antioxidant properties of selected wild plants. International Journal of Science and Research, 4(2), 197-200.
Anosike, C. A., Obidoa, O., & Ezeanyika, L. U. (2012). Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU Journal of Pharmaceutical Sciences, 20(76), 1-7. https://doi.org/10.1186/2008-2231-20-76
Battu, G. R., Ethadi, S. R., Priya, G. V., Priya, K. S., Chandrika, K., Rao, A. V., & Reddy, S. O. (2011). Evaluation of antioxidant and anti-inflammatory activity of Euphorbia heyneana Spreng. Asian Pacific Journal of Tropical Biomedicine, 1(2), 191-194. https://doi.org/10.1016/S2221-1691(11)60154-8
Bentahar, A., Khennouf, S., Bouaziz, A., & Djidel, S. (2012). Evaluation of polyphenol content and antioxidant activity of aqueous extracts of Ceratonia siliqua L. and Ruta montana L. In Proceeding of the 2nd African Congress on Biology & Health University Ferhat Abbas Setif1(pp. 30-34). Biological & Pharmacological Activities of Natural Substances.
Biswas, S. K. (2016). Does the interdependence between oxidative stress and inflammation explain the antioxidant paradox?. Oxidative Medicine and Cellular Longevity, 2016, 1-9. https://doi.org/10.1155/2016/5698931
Boutennoun, H., Boussoufe, L., Kebieche, M., Al-Qaoud, K., & Madani, K. (2019). Phenolics content, antiproliferative and antioxidant activities of Algerian Malva sylvestris. European Journal of Biological Research, 9(1), 10-19. http://dx.doi.org/10.5281/zenodo.2545914
Boutennoun, H., Boussouf, L., Rawashdeh, A., Al-Qaoud, K., Abdelhafez, S., Kebieche, M., & Madani, K. (2017). In vitro cytotoxic and antioxidant activities of phenolic components of Algerian Achillea odorata leaves. Arabian Journal of Chemistry, 10(3), 403-409. https://doi.org/10.1016/j.arabjc.2014.05.013
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
Carvalho, M., Ferreira, P. J., Mendes, V. S., Silva, R., Pereira, J. A., Jerónimo, C., & Silva, B. M. (2010). Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. Food and Chemical Toxicology, 48(1), 441-447. https://doi.org/10.1016/j.fct.2009.10.043
Custódio, L., Fernandes, E., Escapa, A. L., López-Avilés, S., Fajardo, A., Aligué, R., Alberício, F., & Romano, A. (2009). Antioxidant activity and in vitro inhibition of tumor cell growth by leaf extracts from the carob tree (Ceratonia siliqua). Pharmaceutical Biology, 47(8), 721-728. https://doi.org/10.1080/13880200902936891
Custódio, L., Patarra, J., Alberício, F., Neng, N. R., Nogueira, J. M. F., & Romano, A. (2015). In vitro antioxidant and inhibitory activity of water decoctions of carob tree (Ceratonia siliqua L.) on cholinesterases, α-amylase and α-glucosidase. Natural Product Research, 29(22), 2155-2159. https://doi.org/10.1080/14786419.2014.996147
Dallali, S., Aloui, F., Selmi, H., & Sebei, H. (2018). Comparison of the chemical composition and the antioxidant activity of the leaves of Carob tree (Ceratonia siliqua L.) collected in three sites of Djebel Zaghouan (Tunisia). Journal of New Sciences Agriculture and Biotechnology, 21, 3429-3438.
Ghanemi, F. Z., & Belarbi, M. (2021). Phytochemistry and Pharmacology of Ceratonia siliqua L. leaves. Journal of Natural Product Research and Applications, 1(1), 69-82. https://doi.org/10.46325/jnpra.v1i01.7
Gregoriou, G., Neophytou, C. M., Vasincu, A., Gregoriou, Y., Hadjipakkou, H., Pinakoulaki, E., Christodoulou, M. C., Ioannou, G. D., Stavrou, I. J., Christou, A., Kapnissi-Christodoulou, C.P., Aigner, S., Stuppner, H., Kakas, A., & Constantinou, A. I. (2021). Anti-cancer activity and phenolic content of extracts derived from Cypriot carob (Ceratonia siliqua L.) pods using different solvents. Molecules, 26(16), 1-22. https://doi.org/10.3390/molecules26165017
Gurib-Fakim, A. (2006). Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular Aspects of Medicine, 27(1), 1-93. https://doi.org/10.1016/j.mam.2005.07.008
Heilerová, Ľ., Bučková, M., Tarapčík, P., Šilhár, S., & Labuda, J. (2003). Comparison of antioxidative activity data for aqueous extracts of lemon balm (Melissa officinalis L.), oregano (Origanum vulgare L.), thyme (Thymus vulgaris L.), and agrimony (Agrimonia eupatoria L.) obtained by conventional methods and the DNA-based biosensor. Czech Journal of Food Sciences, 21(2), 78-84. https://doi.org/10.17221/3480-CJFS
Hinneburg, I., Dorman, H. J. D., & Hiltunen, R. (2006). Antioxidant activities of extracts from selected culinary herbs and spices. Food Chemistry, 97(1), 122-129. https://doi.org/10.1016/j.foodchem.2005.03.028
Hsouna, A. B., Alayed, A. S., & Abdallah, E. M. (2012). Evaluation of antimicrobial activities of crude methanolic extract of pods of Ceratonia siliqua L. against some pathogens and spoilage bacteria. African Journal of Microbiology Research, 6(14), 3480-3484. https://doi.org/10.5897/AJMR11.1613
Hsouna, A. B., Saoudi, M., Trigui, M., Jamoussi, K., Boudawara, T., Jaoua, S., & El Feki, A. (2011). Characterization of bioactive compounds and ameliorative effects of Ceratonia siliqua leaf extract against CCl4 induced hepatic oxidative damage and renal failure in rats. Food and Chemical Toxicology, 49(12), 3183-3191. https://doi.org/10.1016/j.fct.2011.09.034
Huang, D.-J., Lin, C.-D., I. N., Chen,H.-J.,&Lin, Y.-H. (2004). Antioxidant and antiproliferative activities of sweet potato (Ipomoea batatas [L.] LamTainong 57') constituents. Botanical Bulletin of Academia Sinica, 45, 179-186.
Islam, M. S., Yoshimoto, M., Ishiguro, K., Okuno, S., & Yamakawa, O. (2003). Effect of artificial shading and temperature on radical scavenging activity and polyphenolic composition in sweetpotato (Ipomoea batatas L.) leaves. Journal of the American Society for Horticultural Science, 128(2), 182-187.https://doi.org/10.21273/JASHS.128.2.0182
Kar, B., Kumar, R. B. S., Karmakar, I., Dola, N., Bala, A., Mazumder, U. K., & Hadar, P. K. (2012). Antioxidant and in vitro anti-inflammatory activities of Mimusops elengi leaves. Asian Pacific Journal of Tropical Biomedicine, 2(2), 976-980. https://doi.org/10.1016/S2221-1691(12)60346-3
Lachkar, N., Al-Sobarry, M., El Hajaji, H., Lamkinsi, T., Lachkar, M., Cherrah, Y., & Alaoui, K. (2016). Anti-inflammatory and antioxidant effect of Ceratonia siliqua L. methanol barks extract. Journal of Chemicaland Pharmaceutical Research, 8(3), 202-210.
Leelaprakash, G., & Dass, S. M. (2011). In vitro anti-inflammatory activity of methanol extract of Enicostemma axillare. International Journal of Drug Development and Research, 3(3), 189-196.
Locatelli, M., Travaglia, F., Coïsson, J. D., Martelli, A., Stévigny, C., & Arlorio, M. (2010). Total antioxidant activity of hazelnut skin (Nocciola Piemonte PGI): Impact of different roasting conditions. Food Chemistry, 119(4), 1647-1655. https://doi.org/10.1016/j.foodchem.2009.08.048
Loizzo, M. R., Said, A., Tundis, R., Hawas, U. W., Rashed, K., Menichini, F., Frega, N. G., & Menichini, F. (2009). Antioxidant and antiproliferative activity of Diospyros lotus L. extract and isolated compounds. Plant Foods for Human Nutrition, 64, 264-270. https://doi.org/10.1007/s11130-009-0133-0
Makris, D. P., & Kefalas, P. (2004). Carob pods (Ceratonia siliqua L.) as a source of polyphenolic antioxidants. Food Technology and Biotechnology, 42(2), 105-108. https://hrcak.srce.hr/110800
Mariod, A. A., Ibrahim, R. M., Ismail, M., & Ismail, N. (2009). Antioxidant activity and phenolic content of phenolic rich fractions obtained from black cumin (Nigella sativa) seedcake. Food Chemistry, 116(1), 306-312. https://doi.org/10.1016/j.foodchem.2009.02.051
Mathew, S., & Abraham, T. E. (2006). In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food and Chemical Toxicology, 44(2), 198-206. https://doi.org/10.1016/j.fct.2005.06.013
Miguel, M. G., Nunes, S., Dandlen, S. A., Cavaco, A. M., & Antunes, M. D. (2010). Phenols and antioxidant activity of hydro-alcoholic extracts of propolis from Algarve, South of Portugal. Food and Chemical Toxicology, 48(12), 3418-3423. https://doi.org/10.1016/j.fct.2010.09.014
Mizushima, Y., & Kobayashi, M. (1968). Interaction of anti‐inflammatory drugs with serum proteins, especially with some biologically active proteins. Journal of Pharmacy and Pharmacology, 20(3), 169-173. https://doi.org/10.1111/j.2042-7158.1968.tb09718
Namoune, I., Khettal, B., Assaf, A. M., Elhayek, S., & Arrar, L. (2018). Antioxidant and anti-inflammatory activities of organic and aqueous extracts of Northeast Algerian Marrubium vulgare.Phytothérapie, 16(1), 119-129. https://doi.org/10.3166/phyto-2018-0106
Oyaizu, M. (1986). Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307-315. http://dx.doi.org/10.5264/eiyogakuzashi.44.307
Roseiro, L. B., Duarte, L. C., Oliveira, D. L., Roque, R., Bernardo-Gil, M. G., Martins, A. I., Sepúlveda, C., Almeida, J., Meireles. M., Gírio, F. M., &Rauter, A. P. (2013). Supercritical, ultrasound and conventional extracts from carob (Ceratonia siliqua L.) biomass: Effect on the phenolic profile and antiproliferative activity. Industrial Crops and Products, 47, 132-138. https://doi.org/10.1016/j.indcrop.2013.02.026
Rtibi, K., Selmi, S., Grami, D., Amri, M., Eto, B., El-Benna, J., Sebai, H., &Marzouki, L. (2017). Chemical constituents and pharmacological actions of carob pods and leaves (Ceratonia siliqua L.) on the gastrointestinal tract: A review. Biomedicine & Pharmacotherapy, 93, 522-528. https://doi.org/10.1016/j.biopha.2017.06.088
Rtibi, K., Selmi, S., Jabri, M.-A., Mamadou, G., Limas-Nzouzi, N., Sebai, H., El-Benna, L., Marzouki, L., Eto, B., & Amri, M. (2016). Effects of aqueous extracts from Ceratonia siliqua L. pods on small intestinal motility in rats and jejunal permeability in mice. RSC Advances, 6(50), 44345-44353. https://doi.org/10.1039/C6RA03457H
Shahwar, D., & Raza, M. A. (2012). Antioxidant potential of phenolic extracts of Mimusops elengi. Asian Pacific Journal of Tropical Biomedicine, 2(7), 547-550. https://doi.org/10.1016/S2221-1691(12)60094-X
Shinde, U. A., Phadke, A. S., Nair, A. M., Mungantiwar, A. A., Dikshit, V. J., & Saraf, M. N. (1999). Membrane stabilizing activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia, 70(3), 251-257. https://doi.org/10.1016/S0367-326X(99)00030-1
Sivaraj, C., Sree, N., Arumugam, P., & Gayathri, K. (2017). Anti-inflammatory, antimicrobial and cytotoxic activities of essential oil extracted from Salvia officinalis L. World Journal of Pharmaceutical Sciences, 5(6), 257-263.
Spizzirri, U. G., Abduvakhidov, A., Caputo, P., Crupi, P., Muraglia, M., Rossi, C. O., Clodoveo, M. L., Aiello, F., & Restuccia, D. (2022). Kefir enriched with carob (Ceratonia siliqua L.) leaves extract as a new ingredient during a gluten-free bread-making process. Fermentation, 8(7), 1-14. https://doi.org/10.3390/fermentation8070305
Stanojević, L., Stanković, M., Nikolić, V., Nikolić, L., Ristić, D., Čanadanovic-Brunet, J., & Tumbas, V. (2009). Antioxidant activity and total phenolic and flavonoid contents of Hieracium pilosella L. extracts. Sensors, 9(7), 5702-5714. https://doi.org/10.3390/s90705702
Turkmen, N., Velioglu, Y. S., Sari, F., & Polat, G. (2007). Effect of extraction conditions on measured total polyphenol contents and antioxidant and antibacterial activities of black tea. Molecules, 12(3), 484-496. https://doi.org/10.3390/12030484
Vadivu, R., & Lakshmi, K. S. (2008). In vitro and in vivo anti-inflammatory activity of leaves of Symplocos cochinchinensis (Lour) Moore ssp Laurina. Bangladesh Journal of Pharmacolog, 3(2), 121-124. https://doi.org/10.3329/bjp.v3i2.956
Ydjedd, S., Chaala, M., Richard, G., Kati, D. E., López-Nicolás, R., Fauconnier, M. L., & Louaileche, H. (2017). Assessment of antioxidant potential of phenolic compounds fractions of Algerian Ceratonia siliqua L. pods during ripening stages. International Food Research Journal, 24(5), 2041-2049. https://hdl.handle.net/2268/215392
Yu, J., Ahmedna, M., & Goktepe, I. (2005). Effects of processing methods and extraction solvents on concentration and antioxidant activity of peanut skin phenolics. Food Chemistry, 90(1-2), 199-206. https://doi.org/10.1016/j.foodchem.2004.03.048
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