Isolamento e regeneração de protoplastos de Penicillium brevicompactum - DOI: 10.4025/actascibiolsci.v26i4.1529

Maurílio Antônio Varavalho; Marisa Vieira de Queiroz; Jorge Fernando Pereira; Elza Fernandes de Araújo

doi:10.4025/actascibiolsci.v26i4.1529

Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529

Maurílio Antônio Varavalho Centro Universitario das Faculdades Metropolitanas Unidas
Marisa Vieira de Queiroz UFV
Jorge Fernando Pereira UFV
Elza Fernandes de Araújo UFV

DOI: https://doi.org/10.4025/actascibiolsci.v26i4.1529

Abstract

The isolation and regeneration of fungal protoplasts is a key step for the establishment of transformation systems, electrophoretic karyotype analysis and fusion between strains, all techniques of broad application on improvement programs of filamentous fungi. To establish conditions for the isolation and regeneration of ,em>Penicillium brevicompactum protoplasts, that is an excellent pectinase producer, different lytic enzymes and osmotic stabilizers were tested. P. brevicompactum protoplasts were obtained at a larger scale when their mycelium was digested with 15mg.mL^-1 of Glucanex (Novo Nordisk) and 0.8 mol.L^–1 NaCl as osmotic stabilizer. The best osmotic stabilizer for the regeneration of P. brevicompactum protoplasts was 0.8 mol.L^–1 KCl, with a regeneration frequency of 36.58%. This protocol can be applied in genetic analysis of this Penicillium species which, to date has been poorly characterized

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Author Biography

Marisa Vieira de Queiroz, UFV

possui graduação em Ciências Biológicas- Bacharelado pela Universidade Federal do Rio Grande do Norte (1985), mestrado em Genética e Melhoramento de Plantas pela Universidade de São Paulo (1988) e doutorado em Genética e Melhoramento de Plantas pela Universidade de São Paulo (1993). Atualmente é professora associada da Universidade Federal de Viçosa. Tem experiência na área de Genética, com ênfase em Genética Molecular e de Microorganismos, atuando principalmente nos seguintes temas: genética de fungos, fitopatógenos e produtores de enzimas de interesse industrial, Crinipellis perniciosa, Phaeoisariopsis griseola, Colletotrichum lindemuthianum, Penicillium, elementos transponíveis e micovírus Currículo Lattes

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Published

2008-03-31

How to Cite

Varavalho, M. A., Queiroz, M. V. de, Pereira, J. F., & Araújo, E. F. de. (2008). Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529. Acta Scientiarum. Biological Sciences, 26(4), 475-479. https://doi.org/10.4025/actascibiolsci.v26i4.1529

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Issue

Vol 26 No 4 (2004)

Section

Biology Sciences

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