Development and characterization of microsatellite markers for Tibouchina hatschbachii (Melastomataceae), an endemic and habitat-restricted shrub from Brazil

Fabiano Rodrigo Maia; Patricia Sanae Sujii; Renato Goldenberg; Viviane Silva Pereira; Maria Imaculada Zucchi

doi:10.4025/actascibiolsci.v38i3.31496

Development and characterization of microsatellite markers for Tibouchina hatschbachii (Melastomataceae), an endemic and habitat-restricted shrub from Brazil

Fabiano Rodrigo Maia Universidade Estadual de Campinas
Patricia Sanae Sujii Universidade Estadual de Campinas
Renato Goldenberg Universidade Federal do Paraná
Viviane Silva Pereira Universidade Federal do Paraná
Maria Imaculada Zucchi Agência Paulista de Tecnologia dos Agronegócios

DOI: https://doi.org/10.4025/actascibiolsci.v38i3.31496

Keywords: Melastomataceae, Tibouchina, granite, sandstone, rock outcrops, population genetics, simple sequence repeat (SSR)

Abstract

Tibouchina hatschbachii Wurdack (Melastomataceae) is an autogamous shrub restricted to granite (GO) and sandstone (SO) rock outcrops from subtropical Brazil. We designed primers for the amplification of microsatellite regions for T. hatschbachii, and characterized these primers to estimate genetic diversity parameters and contemporary genetic structure patterns. Eight loci were successfully amplified and were characterized using 70 individuals from three natural populations. Polymorphic information content ranged from 0.200 to 0.772 per locus. All loci were polymorphic, with allele numbers ranging from two to eight. The low degree of polymorphism may be explained by the fact that T. hatschbachii has disjunct populations and a recent genetic bottleneck, and also that it is self-pollinated. The observed and expected heterozygosities ranged from 0.115 to 1.000 and from 0.112 to 0.800, respectively. We observed private alleles in all loci. These are important features that enable us to identify population differentiation and help to us understand gene flow patterns for T. hatschbachii in subtropical Brazil. Eight microsatellite loci from other species of Tibouchina amplified positively in T. hatschbachii.

Downloads

Download data is not yet available.

Author Biography

Fabiano Rodrigo Maia, Universidade Estadual de Campinas

Doutorando do Programa de Pós-Graduação em Biologia Vegetal da Universidade Estadual de Campinas. Desenvolve suas atividades no Instituto de Biologia Vegetal da UNICAMP e no Departamento de Botânica da UFPR. Atualmente atua na área de biologia reprodutiva e da polinização de espécis de Melastomataceae, e também na área de genética de populações destas plantas. Desenvolve seus estudos utilizando marcadores microssatélites plastidiais e nucleares.

Published

2016-12-08

How to Cite

Maia, F. R., Sujii, P. S., Goldenberg, R., Pereira, V. S., & Zucchi, M. I. (2016). Development and characterization of microsatellite markers for Tibouchina hatschbachii (Melastomataceae), an endemic and habitat-restricted shrub from Brazil. Acta Scientiarum. Biological Sciences, 38(3), 327-332. https://doi.org/10.4025/actascibiolsci.v38i3.31496

Download Citation

Issue

Vol 38 No 3 (2016)

Section

Genetics

DECLARATION OF ORIGINALITY AND COPYRIGHTS

I Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal.

The copyrights belong exclusively to the authors. Published content is licensed under Creative Commons Attribution 4.0 (CC BY 4.0) guidelines, which allows sharing (copy and distribution of the material in any medium or format) and adaptation (remix, transform, and build upon the material) for any purpose, even commercially, under the terms of attribution.

Read this link for further information on how to use CC BY 4.0 properly.