Comparação de métodos de cultivo para o diagnóstico laboratorial da tuberculose pulmonar - DOI: 10.4025/actascihealthsci.v27i2.1418

Elisa Keiko Hirayama Takao; Samara R. Nocchi; Vera Lúcia Dias Siqueira; Marco Antonio Cardoso; Maria Luisa Dias Peron; Katiany Rizieri Callefi; Rosilene Fressatti Cardoso

doi:10.4025/actascihealthsci.v27i2.1418

Comparison of culture methods in pulmonary tuberculosis laboratorial diagnosis - DOI: 10.4025/actascihealthsci.v27i2.1418

Authors

Elisa Keiko Hirayama Takao UEM Author
Samara R. Nocchi UEM Author
Vera Lúcia Dias Siqueira UEM Author
Marco Antonio Cardoso UEM Author
Maria Luisa Dias Peron UEM Author
Katiany Rizieri Callefi UEM Author
Rosilene Fressatti Cardoso UEM Author

DOI:

https://doi.org/10.4025/actascihealthsci.v27i2.1418

Keywords:

micobactérias, cultura, diagnóstico, Mycobacterium tuberculosis

Abstract

This study aimed to evaluate different methods for culturing sputum, Ogawa-Kudoh (O - K) and Petroff, using reduced time for decontamination (Petroff - R), expecting to know their effectiveness in tuberculosis laboratory diagnosis. Four hundred and fifty-nine sputum samples of patients with pulmonary symptoms were cultured using O - K and Petroff - R, to be compared with the regular Petroff method. O - K and Petroff - R methods showed efficient sensitivity in isolating Mycobacterium tuberculosis and micobacteria other than tuberculosis, even in samples with negative acid fast smear. Statistics analysis showed similar contamination rate for O - K when compared to Petroff. These data suggest that O-K, being easy to perform, having low costs, presenting low risk of worker contamination and good sensitivity in detecting M. tuberculosis, should be used in clinical laboratories as a good resource at tuberculosis diagnosis

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Author Biography

Rosilene Fressatti Cardoso, UEM

Possui graduação em Farmacia Bioquimica pela Universidade Estadual de Maringá (1984), mestrado em Ciências Biológicas (Biologia Celular) pela Universidade Estadual de Maringá (1994) e doutorado em Farmácia (Análises Clínicas) pela Universidade de São Paulo (2003). Atualmente é professor adjunto da Universidade Estadual de Maringá. Tem experiência na área de Microbiologia, com ênfase em Microbiologia Médica, atuando principalmente nos seguintes temas: Mycobacterium tuberculosis, tuberculose, diagnóstico laboratorial, cultura e diagnóstico Currículo Lattes

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Published

2008-03-26

Issue

Vol. 27 No. 2 (2005)

Section

Clinical Analysis

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How to Cite

Comparison of culture methods in pulmonary tuberculosis laboratorial diagnosis - DOI: 10.4025/actascihealthsci.v27i2.1418. (2008). Acta Scientiarum. Health Sciences, 27(2), 183-188. https://doi.org/10.4025/actascihealthsci.v27i2.1418