<b>Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental β-TCP compared to other bone substitutes

  • Noala Vicensoto Moreira Milhan Universidade Estadual Paulista
  • Isabel Chaves Silva Carvalho Universidade Estadual Paulista
  • Renata Falchete do Prado Universidade Estadual Paulista
  • Eliandra de Sousa Trichês Universidade Federal de São Paulo
  • Carlos Henrique Ribeiro Camargo Universidade Estadual Paulista
  • Samira Esteves Afonso Camargo Universidade Estadual Paulista

Resumo

The aim of this study was to evaluate the indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental beta tri-calcium phosphate (experimental β-TCP) compared with two other bone substitutes: bovine hydroxyapatite (HA) (Bio-Oss® - Geistlich) and beta tri-calcium phosphate (β-TCP - Bionnovation). The cell viability and genotoxicity were measured by MTT and MNT assay, respectively. The indicators of osteogenesis were analyzed by alkaline phosphatase activity, total protein content, and calcium deposition. The MTT and MNT assay showed that none of the tested materials was cytotoxic nor genotoxic. Concerning the indicators of osteogenesis, it was observed that cells in contact with all the materials were able to induce the osteogenesis and this process was influenced by the period of the cell culture in contact with bone substitutes. Based on the results of this study, it was concluded that this experimental β-TCP appears to be a promising material as a bone substitute.

 

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Biografia do Autor

Noala Vicensoto Moreira Milhan, Universidade Estadual Paulista
Departamento de Biociências e Diagnóstico Bucal
Publicado
2017-05-31
Como Citar
Milhan, N. V. M., Carvalho, I. C. S., Prado, R. F. do, Trichês, E. de S., Camargo, C. H. R., & Camargo, S. E. A. (2017). <b&gt;Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental β-TCP compared to other bone substitutes. Acta Scientiarum. Health Sciences, 39(1), 97-105. https://doi.org/10.4025/actascihealthsci.v39i1.29788
Seção
Odontologia

0.3
2019CiteScore
 
 
8th percentile
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0.3
2019CiteScore
 
 
8th percentile
Powered by  Scopus