Investigation of Alphavirus of public health interest in equines in Foz do Iguaçu, the Triple Frontier region (Brazil-Paraguay-Argentina)

Investigation of Alphavirus

  • Alexander Welker Biondo
  • Tatiana Carneiro Rocha
  • Mario Antonio Navarro Silva
  • Thállitha Samih Wischral Jayme Vieira
  • Alexander Welker Biondo
  • Ivan Roque de Barros Filho
  • Robson Michael Delai
  • André de Souza Leandro
  • Beatriz Böger UFPR
  • Walfrido Kühl Svoboda
  • Eliane Carneiro

Resumo

Introduction: The Alphavirus genus encompasses arboviruses of great importance to public health. These viruses presents zoonotic potential and are associated with infections in humans and other vertebrates, including equines. Reverse transcription followed by polymerase chain reaction (RT-PCR) allows the early recognition of Alphavirus circulation, which is crucial for effective preventive measures adoption. This study aimed to investigate the presence of Alphavirus in equines hosts living in close contact with humans and other animals in the city of Foz do Iguaçu (Brazil), which is part of the Triple Frontier region of Brazil, Paraguay and Argentina.Methods: Equines serum samples (n=117) obtained in 2015 and 2016 were subjected to RT-PCR to screen for the presence of the viral genome.Results: All samples were negative for the presence of Alphavirus.Conclusions: These data indicate that the equines examined were not infected and did not represent a risk of viral transmission to humans or other animals during the period in which the samples were collected. This information is relevant because these animals may act as sentinels and urban disseminators of pathogens. Furthermore, active surveillance activities may also contribute to the reduction of negative impacts on the main economic activities of this region.

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Referências

BATISTA, P.M.; ANDREOTTI, R.; ALMEIDA, P.S.; MARQUES, A.C.; RODRIGUES, S.G.; CHIANG, J.O.; VASCONCELOS, P.F.C. Detection of arboviruses of public health interest in free-living New World primates (Sapajus spp.; Alouatta caraya) captured in Mato Grosso do Sul, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 46, p. 684-90, 2013.

BRASIL. Ministério da Saúde. Boletim Epidemiológico nº 24 - Monitoramento dos casos de dengue, febre de chikungunya e febre pelo vírus Zika até a Semana Epidemiológica 17 June 2018. 2018. Available in: http://portalarquivos2.saude.gov.br/images/pdf/2018/junho/11/2018-022.pdf. Accessed 15 Jun 2018.

BRAZIL. Ministério do Turismo. Cidades do Sul estão entre as 10 mais visitadas do País. 2017. Available in: http://www.turismo.gov.br/%C3%BAltimas-not%C3%ADcias/8101-regi%C3%A3o-sul-entre-as-10-mais-do-turismo-internacional.html. Accessed 10 Jun 2018.

BRONZONI, R.V.; MORELI, M.L.; CRUZ, A.C.; FIGUEIREDO, L.T. Multiplex nested PCR for Brazilian Alphavirus diagnosis. Transactions of the Royal Society of Tropical Medicine and Hygiene. v.98, p.456-61, 2004.

BRUNO-LOBO, G.G.; BRUNO-LOBO, M.; TRAVASSOS, J.; PINHEIRO, F.; PAZIN, I. P. Estudos sobre arbovírus III. Isolamento de um vírus sorologicamente relacionado ao sub-grupo Western-Sindbis de um caso de encefalomielite eqüina ocorrido no Rio de Janeiro. Annals of Microbiology, v. 9, p. 183-95, 1961.

CAMPOS, K.F.; OLIVEIRA, C.H.; REIS, A.B.; YAMASAKI, E.M.; BRITO, M.F.; ANDRADE, S.J.; DUARTE, M.D.; BARBOSA, J.D. Surto de encefalomielite equina Leste na Ilha de Marajó, Pará. Pesquisa Veterinária Brasileira, v. 33, p. 443-8, 2013.

CASSEB, A. R.; BRITO, T. C.; SILVA, M. R.; CHIANG, J. O.; MARTINS, L. C.; SILVA, S. P.; HENRIQUES, D. F.; CASSEB, L. M. N.; VASCONCELOS, P. F. C. Prevalence of antibodies to equine alphaviruses in the State of Pará, Brazil. Arquivos do Instituto Biológico, v. 83, p. 1-5,2016.

CASSEB, A.R.; CHIANG, J.O.; MARTINS, L.C.; SILVA, S.P.; HENRIQUES, D.F.; CASSEB, L.M.; VASCONCELOS, P.F.C. Alphavirus serosurvey in domestic herbivores in Pará State, Brazilian Amazon. Revista Pan-Amazônica de Saúde, v.3, p.43-8, 2013.
COIMBRA, T.L.; SANTOS, C.L.; SUZUKI, A.; PETRELLA, S.M.; BISORDI, I.; NAGAMORI, A. H.; MARTI, A.T.; SANTOS, R.N.; FIALHO, D.M.; LAVIGNE, S., BUZZAR., M.R.; ROCCO, I.M. Mayaro virus: imported cases of human infection in São Paulo State, Brazil. Instituto de Medicina Tropical de São Paulo, v. 4, p. 9:221-4, 2007.

CUNHA, E.M.; VILLALOBOS, E.M.; NASSAR, A.F.; LARA, M.C.; PERES, N.F.; PALAZZO, J.P. SILVA, A.; STEFANO, E.; PINO, F.A. Prevalência de anticorpos contra agentes virais em equídeos no sul do estado de São Paulo, Instituto Biológico, Centro de Pesquisa e Desenvolvimento de Sanidade Animal. Arquivos do Instituto Biológico, v. 76, p. 165-71, 2009.

DÍAZ, L.A.; DÍAZ, M.P.; ALMIRÓN, W.R.; CONTIGIANI, M.S. Infection by UNA virus (Alphavirus; Togaviridae) and risk factor analysis in black howler monkeys (Alouatta caraya) from Paraguay and Argentina. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 101, p. 1039-41, 2007.

DÍAZ, L.A.; SPINSANTI, L.I.; ALMIRÓN, W.R.; CONTIGIANI, M.S. Una Virus: first report of human infection in Argentina. Revista do Instituto de Medicina Tropical de São Paulo, v. 45, p. 109-10, 2003.

FERNÁNDEZ, Z.; RICHARTZ, R.; TRAVASSOS, D.A.; ROSA, A.; SOCCOL, V. T. Identificação do vírus causador da encefalomielite eqüina, Paraná, Brasil. Revista de Saúde Pública, v. 34, n. 232-5, 2000.

GUBLER, D.J. The global emergence/resurgence of arboviral diseases as public health problems. Archives of Medical Research, v. 33, p. 330-42, 2002.

HEINEMANN, M.B.; SOUZA, M.C.; CORTEZ, A.; FERREIRA, F.; HOMEM, F.; FERREIRA-NETO, J.S.; SOARES, R.M.; CUNHA, E.M.S.; RICHTZENHAIN, L. J. Soroprevalência da encefalomielite equina do leste e do oeste no Município de Uruará, PA, Brasil. Brazilian Journal of Veterinary Research and Animal Science, v. 43, p.137-9, 2006.

INTERNATIONAL COMMITTEE ON THE TAXONOMY OF VIRUSES (ICTV). Virus Taxonomy: 2017 release. 2017. Available in: https://talk.ictvonline.org /taxonomy/. Accessed 10 Aug 2018.

IVERSSON, L.B.; ROSA, A.P.; ROSA, J.T. Serological studies in research on arbovirus antibodies in the human population of the Ribeira Valley region. II - Survey of patients of Pariquera-Açú Regional Hospital, 1980. Revista de Saúde Pública, v. 15, p. 587-602, 1981.

MELO, R. M.; CAVALCANTI, R. C.; VILLALOBOS, E. M.; CUNHA, E. M.; LARA, M. C.; AGUIAR, D.M. Ocorrência de equídeos soropositivos para os vírus das encefalomielites e anemia infecciosa no estado do Mato Grosso. Arquivos do Instituto Biológico, v.79, p.169-75, 2012.

PAN AMERICAN HEALTH ORGANIZATION (PAHO). Number of Reported Cases of Chikungunya Fever in the Americas, by Country or Territory. 2015. Available in: https://www.paho.org/hq/dmdocuments/2015/2015-may-15-cha-CHIKV-cumulative-cases.pdf. Accessed 25 Jun 2018.

PAN AMERICAN HEALTH ORGANIZATION (PAHO). Number of Reported Cases of Chikungunya Fever in the Americas, by Country or Territory. 2016. Available in: https://www.paho.org/hq/dmdocuments/2016/2015-cha-CHIKV-cumulative-cases-update.pdf. Accessed 25 Jun 2018.

PAN AMERICAN HEALTH ORGANIZATION (PAHO). Number of Reported Cases of Chikungunya Fever in the Americas, by Country or Territory. 2017. Available in: https://www.paho.org/hq/dmdocuments/2017/2017-dec-22-phe-CHIKV-cases-ew-51.pdf. Accessed 25 Jun 2018.

PAUVOLID-CORREA, A.; JULIANO, R.S.; CAMPOS, Z.; VELEZ, J.; NOGUEIRA, R.M.; KOMAR, N. Neutralising antibodies for Mayaro virus in Pantanal, Brazil. Memórias do Instituto Oswaldo Cruz, v.110, p.125-33, 2015.

PFEFFER, M.; PROEBSTER, B.; KINNEY, R.M.; KAADEN, O. R. Genus-specific detection of alphaviruses by a semi-nested reverse transcription-polymerase chain reaction. American Journal of Tropical Medicine and Hygiene, v. 57, p.709-18, 1997.

PISANO, M.B.; DANTUR, M.J.; RÉ, V.E.; DÍAZ, L.A.; FARÍAS, A.; SÁNCHES-SECO, M.P.; TENORIO, A.; ALMIRÓN, W.R.; CONTIGIANI, W.S. Cocirculation of Rio Negro Virus (RNV) and Pixuna Virus (PIXV) in Tucumán province, Argentina. Tropical Medicine & International Health, v. 15, p. 865-8, 2010B.

PISANO, M.B.; ORIA, G.; BESKOW, G.; AGUILAR, J.; KONIGHEIM, B.; CACACE, M.L.; AGUIRRE, L.; STEIN, M.; CONTIGIANI, M. S. Venezuelan Equine Encephalitis Viruses (VEEV) in Argentina: Serological Evidence of Human Infection. PLOS Neglected Tropical Diseases, v. 7, p. 1-7, 2013.

PISANO, M.B.; RÉ, V.E.; DÍAZ, L.A.; FARÍAS, A.; STEIN, M.; SÁNCHEZ-SECO, M.P.; TENORIO, A.; ALMIRÓN, W.R.; CONTIGIANI, W.S. Enzootic Activity of Pixuna and Rio Negro Viruses (Venezuelan Equine Encephalitis complex) in a Neotropical Region of Argentina. Vector-Borne and Zoonotic Diseases, v. 10, p. 199-201, 2010A.

PISANO, M.B.; SPINSANTI, L.I.; DÍAZ, L.A.; FARÍAS, A.A.; ALMIRÓN, W.R.; RÉ, V.E. CONTIGIANI, M.S. First detection of Rio Negro virus (Venezuelan Equine Encephalitis Complex Subtype VI) in Córdoba, Argentina. Memórias do Instituto Oswaldo Cruz, v. 107, p. 125-8, 2012.

PREFEITURA MUNICIPAL DE FOZ DO IGUAÇU. Dados Socioeconômicos de Foz do Iguaçu. 2017. Available in: http://www.pmfi.pr.gov.br. Accessed 10 Jun 2018.

SÁNCHEZ-SECO, M.P.; ROSARIO, D.; QUIROZ, E.; GUZMÁN, G.; TENORIO, A. A generic nested-RT-PCR followed by sequencing for detection and identification of members of the Alphavirus genus. Journal of Virological Methods, v. 95, p.153-61, 2001.

SILVA, M.L.; GALIZA, G.J.; DANTAS, A.F.; OLIVEIRA, R.N.; IAMAMOTO, K.; ACHKAR, S.M.; RIET-CORREA, F. Outbreaks of Eastern equine encephalitis in northeastern Brazil. Journal of Veterinary Diagnostic Investigation, v. 23, p. 570-5, 2011.

VASCONCELOS, P.; TRAVASSOS DA ROSA, A.P.; PINHEIRO, F.P. Arboviruses pathogenic for man in Brazil. In: Travassos da Rosa AP, Vasconcelos P, Travassos da Rosa JF, editors. An overview of arbovirology in Brazil and neighbouring countries. Belém: Instituto Evandro Chagas; 1998. p. 71-99.

VASCONCELOS, P.F.; TRAVASSOS DA ROSA, J.F.; TRAVASSOS DA ROSA, A.P.; DÉGALLIER, N.; PINHEIRO, F.P.; CARRERA SÁ, G. Epidemiologia das encefalites por arbovírus na Amazônia brasileira. Instituto de Medicina Tropical de São Paulo, v. 3, p. 465-76, 1991.

WEAVER, S. C. Urbanization and geographic expansion of zoonotic arboviral diseases: mechanisms and potential strategies for prevention. Trends in Microbiology, p. 21:360-3, 2013.

WEAVER, S.C.; REISEN, W.K. Present and Future Arboviral Threats. Antiviral Research, v. 85, p. 328-45, 2010.

WOOLHOUSE, M.E.; GOWTAGE-SEQUERIA, S. Host range and emerging and reemerging pathogens. Emerging Infectious Diseases, v. 11, p. 1842-7, 2005.

ZUCHI, N.; HEINEN, L.B.; SANTOS, M.A.; PEREIRA, F.C.; SLHESSARENKO, R. D. Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil. Memórias do Instituto Oswaldo Cruz, v. 109, p. 820-3, 2014.
Publicado
2025-05-03
Como Citar
Welker Biondo , A., Carneiro Rocha, T., Antonio Navarro Silva , M., Samih Wischral Jayme Vieira, T., Welker Biondo , A., Roque de Barros Filho , I., Michael Delai , R., de Souza Leandro , A., Böger, B., Kühl Svoboda , W., & Carneiro , E. (2025). Investigation of Alphavirus of public health interest in equines in Foz do Iguaçu, the Triple Frontier region (Brazil-Paraguay-Argentina). Revista De Ciência Veterinária E Saúde Pública, 12(1), 91-104. https://doi.org/10.4025/revcivet.v12i1.56232