Royal jelly production with queens produced by single and double grafting in Africanized honeybee colonies

Pedro Rosa Santos; Tuan Henrique Smielevski de Souza; Diogo Francisco Rossoni; Vagner de Alencar Arnaut de Toledo

doi:10.4025/actascianimsci.v41i1.45670

Pedro Rosa Santos Universidade Estadual de Maringá
Tuan Henrique Smielevski de Souza Universidade Estadual de Maringá
Diogo Francisco Rossoni Universidade Estadual de Maringá
Vagner de Alencar Arnaut de Toledo Universidade Estadual de Maringá http://orcid.org/0000-0003-1814-9703

DOI: https://doi.org/10.4025/actascianimsci.v41i1.45670

Resumo

The objective of this research was to evaluate royal jelly production from Africanized honeybee queens of different lineages (lineage selected for honey production, lineage selected for royal jelly production and unselected) produced by single and double grafting and to compare royal jelly production among their offspring. Data were tested by double factorial analysis of variance and the means were compared by Tukey test at 5%. The parameters evaluated were: queen weight at emergence, percentage of larvae acceptance in the upper and lower bars, royal jelly per cup (mg) and royal jelly per colony/collection (g). Queens selected for honey presented greater weight at emergence, while the unselected queens were the lightest. Double grafting was better than simple grafting, since the queens were born 2.38% heavier. There was no difference (p > 0.05) in relation to royal jelly production according to neither the lineage nor even comparing the method by which the queens were produced. The cost of labor to produce queens by double grafting was much higher, as by the simple grafting about 170% more queens were born. The potential of Africanized honeybees with adequate production management and favorable environmental conditions favors the production of royal jelly.

Downloads

Não há dados estatísticos.

Referências

Alber, M. A. (1965). A study of queen-rearing methods. Bee World, 46(1), 25-31. doi: 10.1080/0005772X.1965.11095326.

Alqarni, A. S., Balhareth, H. M., & Owayss, A. A. (2013). Queen morphometric and reproductive characters of Apis mellifera jemenitica, a native honey bee to Saudi Arabia. Bulletin of Insectology, 66(2), 239-244.

Baitala, T. V., Faquinello, P., Toledo, V. A. A., Mangolin, C. A., Martins, E. N., & Ruvolo-Takasusuki, M. C. C. (2010). Potential use of major royal jelly proteins (MRJPs) as molecular markers for royal jelly production in Africanized honeybee colonies. Apidologie, 41(2), 160-168. doi: 10.1051/apido/2009069.

Baitala, T. V., Nakasugui, L. F., Cantagalli, L. B., Toledo, V. A. A., & Ruvolo-Takasusuki, M. C. C. (2013). Major royal jelly protein-3 polymorphism in Africanized honeybee colonies producing royal jelly. Magistra, 25(1), 30-36.

Berger, B., Poiani, S. B., & Cruz-Landim, C. (2016). Beekeeping practice: effects of Apis mellifera virgin queen management on ovary development. Apidologie, 47(4), 589-595. doi: 10.1007/s13592-015-0404-8.

Cao, L.-F., Zheng, H.-Q., Pirk, C. W., Hu, F.-L., & Xu, Z.-W. (2016). High royal jelly-producing honeybees (Apis mellifera ligustica)(Hymenoptera: Apidae) in China. Journal of Economic Entomology, 109(2), 510-514. doi: 10.1093/jee/tow013.

Delaplane, K. S., & Harbo, J. R. (1988). A re-examination of double grafting. American Bee Journal, 128(6), 439-440.

Doolittle, G. M. (1889). Scientific queen rearing. Chicago, IL: Thomas G Newman & Son.

Faquinello, P., Toledo, V. A. A., Martins, E. N., Oliveira, C. A. L., Sereia, M. J., Costa-Maia, F. M., & Ruvolo-Takasusuki, M. (2011). Parameters for royal jelly production in Africanized honeybees. Sociobiology, 57(3), 495-509.

Harano, K.-i., Sasaki, M., & Sasaki, K. (2007). Effects of reproductive state on rhythmicity, locomotor activity and body weight in the European honeybee, Apis mellifera queens (Hymenoptera, Apini). Sociobiology, 50(1), 189-200.

Kamakura, M. (2011). Royalactin induces queen differentiation in honeybees. Nature, 473(7348), 478. doi: 10.1038/nature10093.

Khan, A. S., Matos, V. D., & Lima, P. V. P. S. (2009). Desempenho da apicultura no estado do Ceará: competitividade, nível tecnológico e fatores condicionantes. Revista de Economia e Sociologia Rural, 47(3), 651-676. doi: 10.1590/S0103-20032009000300006.

Meixner, M. D., Büchler, R., Costa, C., Francis, R. M., Hatjina, F., Kryger, P., ... Carreck, N. L. (2014). Honey bee genotypes and the environment. Journal of Apicultural Research, 53(2), 183-187. doi: 10.3896/IBRA.1.53.2.01.

Metorima, F. N., Costa-Maia, F. M., Halak, A. L., Parpinelli, R. S., & Toledo, V. A. A. (2015). Morphometric measurements of Africanized honeybee queens kept in an incubator or in queen banking. Acta Scientiarum. Animal Sciences, 37(1), 91-96. doi: 10.4025/actascianimsci.v37i1.24006.

Montagner, H. (1962). Influence de la technique du double greffage sur le développement des reines de Apis mellifica. Insectes Sociaux, 9(1), 91-99.

Mouro, G. F., & Toledo, V. A. A. (2004). Evaluation of Apis mellifera Carniolan and Africanized honey bees in royal jelly production. Brazilian Archives of Biology and Technology, 47(3), 469-476.

Parpinelli, R. S., Ruvolo-Takasusuki, M. C. C., & Toledo, V. A. A. (2014). MRJP microsatellite markers in africanized Apis mellifera colonies selected on the basis of royal jelly production. Genetics and Molecular Research, 13(3), 6724. doi: 10.4238/2014.August.28.16.

R-Core-Team. (2016). R: A language and environment for statistical computing. Viena, AT: R. Foundation for Satatical Computing.

Schafaschek, T. P., Hickel, E. R., Pereira, H. L., Oliveira, C. A. L., & Toledo, V. d. A. A. (2016). Performance of Africanized honeybee colonies settled by queens selected for different traits. Acta Scientiarum. Animal Sciences, 38(1), 91-100. doi: 10.4025/actascianimsci.v38i1.26840.

Stuchi, A. L. P. B., Toledo, V. A. A., Lopes, D. A., Cantagalli, L. B., & Ruvolo-Takasusuki, M. C. C. (2014). Molecular marker to identify two stingless bee species: Tetragonisca angustula and Tetragonisca fiebrigi (Hymenoptera, Meliponinae). Sociobiology, 59(1), 123-134.

Terada, Y., Garofalo, C. A., & Sakagami, S. F. (1975). Age-survival curves for workers of two eusocial bees (Apis mellifera and Plebeia droryana) in a subtropical climate, with notes on worker polyethism in P. droryana. Journal of Apicultural Research, 14(3-4), 161-170. doi: 10.1080/00218839.1975.11099821.

Toledo, V. A. A., Alves, E. M., Oliveira, J. R., Ruvolo-Takasusuki, M. C. C., & Faquinello, P. (2010). Produção de geleia real em colônias de abelhas africanizadas considerando diferentes suplementos proteicos e a influência de fatores ambientais. Acta Scientiarum. Animal Sciences, 32(1), 93-100. doi: 10.4025/actascianimsci.v32i1.6836.

Toledo, V. A. A., & Mouro, G. F. (2005). Produção de geleia real com abelhas africanizadas selecionadas e cárnicas híbridas. Revista Brasileira de Zootecnia, 34(6), 2085-2092. doi: 10.1590/S1516-35982005000600034.