Laccase and lignin peroxidase production and decolorization of xenobiotic dyes by species of Polyporaceae (Basidiomycota) from South America

Autores

Palavras-chave:

Bioremediation, Congo red, Crystal violet, Ligninolytic enzymes, RBBR

Resumo

Polyporaceae are known as wood decaying fungi with enzymatic production that plays an important role in environmental processes. Laccase (Lac) and lignin peroxidase (LiP) are two of the most explored enzymes and show significant industrial importance in degrading textile dyes, including Remazol Brilliant Blue R (RBBR), Crystal Violet (CV) and Congo Red (CR) that are some of the most commonly tested. Six strains representing six species of Hexagonia and Trametes (Polyporaceae) from South America were selected to evaluate Lac and LiP production and degradation of these dyes. The dataset of rDNA ITS confirmed the identity of the species, the amplification using LccF–LccR and Lip1–Lip2 primers detected Lac and LiP gene fragments in four and five strains, respectively, and the biostimulation essays of Lac and LiP showed the production of enzymes by all strains, being T. versicolor (VRTO 1064) and H. hydnoides (URM 9027) the best producers of Lac (117.839 U L-1) and LiP (109.370 U L-1), respectively. For RBBR, T. villosa (URM 8022) presented the best results (84.18 %). CR decolorization ranged from 61.37% to 68.74% by five strains, with lower results for T. lactinea (URM 8350) (19.9 %). Finally, T. sanguinea (URM 8774) had the best results for CV (85.06 %). Trametes lactinea (URM 8350), T. versicolor (VRTO 1064), and T. villosa (URM 8022) did not decolorize CV. Our results highlight the underexplored enzymatic potential of strains from South America and show that the strains here studied are promising alternatives to decolorize industrial textile dyes.

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2025-11-10

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Oliveira, V. R. T. de, Wanderley, S. S. ., Santos, C., Gibertoni, T. B. ., & Alvarenga, R. L. M. . (2025). Laccase and lignin peroxidase production and decolorization of xenobiotic dyes by species of Polyporaceae (Basidiomycota) from South America. Acta Scientiarum. Technology, 48(1), e73470. Recuperado de https://periodicos.uem.br/actascitechnol/index.php/ActaSciTechnol/article/view/73470

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v. 48 (2026): Publicação contínua

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Biotecnologia

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Copyright (c) 2026 Virton Rodrigo Targino de Oliveira, Samantha Seehagen Wanderley, Cledir Santos, Tatiana Baptista Gibertoni, Renato Lúcio Mendes Alvarenga (Autor)

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