Mapping and validation of molecular markers of genes Dt1 and Dt2 to determine the type of stem growth in soybean

Dorival Vicente; Ivan Schuster; Fabiane Lazzari; Jonas Pedro Dolfini Paranzini; Marco Antonio Rott de Oliveira; Cássio Egídio Cavenaghi Prete

doi:10.4025/actasciagron.v38i1.26216

Mapping and validation of molecular markers of genes Dt1 and Dt2 to determine the type of stem growth in soybean

Dorival Vicente Expo Grain, Comércio de Sementes Ltda.
Ivan Schuster Cooperativa Central de Pesquisa Agrícola
Fabiane Lazzari Cooperativa Central de Pesquisa Agrícola
Jonas Pedro Dolfini Paranzini Cooperativa Central de Pesquisa Agrícola
Marco Antonio Rott de Oliveira Cooperativa Central de Pesquisa Agrícola
Cássio Egídio Cavenaghi Prete Universidade Estadual de Londrina

DOI: https://doi.org/10.4025/actasciagron.v38i1.26216

Abstract

The type of stem growth in soybean is a distinguishing feature of cultivars. The genes Dt1 and Dt2 affect the termination of the stem, and the types of growth are classified as determinate, semi-determinate and indeterminate. Phenotypic characterization of the type of growth is complex and is occasionally erroneously described. The objective of this study was to map and validate molecular markers to classify the type of soybean growth to facilitate the description of cultivars and genotypic selection. Two populations were used for mapping and validation of molecular markers F_2:3: T 117 (semi-determinate growth type) x Igra RA 518 RR (indeterminate growth type) and CD 235RR (determinate growth type) x Igra RA 518 RR. The study demonstrates that the association of the molecular marker with the gene GmTFL1b was efficient in the classification of soybean growth types. The marker sat_064 is connected to the Dt2 gene, which is located in the Liaison Group G of the consensus map of soybeans with a recombination frequency of 19.4%. Molecular markers for Dt1 and Dt2 genes efficiently described the genotypes of soybean stem growth and selection.

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Author Biography

Dorival Vicente, Expo Grain, Comércio de Sementes Ltda.

Atua na área de engenharia agronômica desde 1980. Possui graduação em Agronomia pela UNESP Campus Jaboticabal (1980), curso de aperfeiçoamento em Defensivos Agrícolas pela Universidade Federal de Viçosa e ABEAS (1984), mestrado em Agronomia em área de concentração de Melhoramento Genético Vegetal pela Universidade Estadual de Maringá (2003) e doutor em Agronomia na Universidade Estadual de Londrina (2012) na área de Fitotecnia (Melhoramento Vegetal e Biotecnologia Aplicada). Foi professor da Faculdade Assis Gurgacz (2006 – 2008). Foi melhorista de soja na COODETEC (1995 - 2013). Trabalha com os seguintes temas: melhoramento genético de soja, biossegurança e controle de plantas daninhas.

Published

2016-01-01

How to Cite

Vicente, D., Schuster, I., Lazzari, F., Paranzini, J. P. D., Oliveira, M. A. R. de, & Prete, C. E. C. (2016). Mapping and validation of molecular markers of genes Dt1 and Dt2 to determine the type of stem growth in soybean. Acta Scientiarum. Agronomy, 38(1), 61-68. https://doi.org/10.4025/actasciagron.v38i1.26216

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Issue

Vol 38 No 1 (2016)

Section

Genetics and Plant Breeding

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