Chitosan application in the induction of water deficit tolerance in maize plants

Lorena Gabriela Almeida; Paulo César Magalhães; Décio Karam; Eder Marcos da  Silva; Amauri Alves  Alvarenga

doi:10.4025/actasciagron.v42i1.42463

Lorena Gabriela Almeida Universidade Federal de Lavras http://orcid.org/0000-0002-2018-2206
Paulo César Magalhães Empresa Brasileira de Pesquisa Agropecuaria
Décio Karam Empresa Brasileira de Pesquisa Agropecuária
Eder Marcos da Silva Universidade Federal de Lavras
Amauri Alves Alvarenga Universidade Federal de Lavras

DOI: https://doi.org/10.4025/actasciagron.v42i1.42463

Abstract

The present research seeks to elucidate the feasibility of chitosan (CHT) in the induction of water deficit tolerance in different maize hybrids, contrasting tolerance to water restriction, tolerance and sensitivity. The maize plants were subjected to water deficit and foliar application of different chitosan doses (60, 100, 140, and 180 mg L^-1) at the pre-flowering growth stage and evaluated during the stress period of fifteen days. To understand the induction behaviour of the tolerance to water restriction, biophysical parameters, such as water potential, relative water content and chlorophyll content, gas exchange, and biochemical assays, were quantified based on the activity of SOD, CAT, APX, and PAL antioxidant enzymes, lipid peroxidation activity and hydrogen peroxide content. Among the treatments, maize plants subjected to chitosan foliar application at a dose of 140 mg L^-1 presented similar behavioural responses to plants under favourable irrigation conditions. Such positive responses are related to the high degree of activity of antioxidant enzymes, gas exchange and low levels of lipid peroxidation and hydrogen peroxide. The results support the potential use of CHT to increase tolerance to water stress.

Downloads

Download data is not yet available.

References

Biemelt, S., Keetman, U., & Albrecht, G. (1998). Re-aeration following hypoxia or anoxia leads to activation of the antioxidative defense system in roots of wheat seedlings. Plant Physiology, 116(2), 651-658.

Buege, J. A., & Aust, S. D. (1978). Microsomal lipid peroxidation methods. Methods in Enzymology, 52(78), 302-310. DOI: 10.1016/S0076-6879(78)52032-6

Chaves, M. M., Costa, J. M., Zarrouk, O., Pinheiros, C., Lopes, C. M., & Pereira, J. S. (2016). Controlling stomatal aperture in semi-arid regions -The dilemma of saving water or being cool? Plant Science, 251, 54-64. DOI: 10.1016/j.plantsci.2016.06.015

Dzung, N. A., Khanh, V. T. P., & Dzung, T. T. (2011). Research on impact of chitosan oligomers on biophysical characteristics, growth, development and drought resistance of coffee. Carbohydrate Polymers, 84(2), 751-755.

Emam, M. M., Khattab, H. E., Helal, N. M., & Deraz, A. E. (2014). Effect of selenium and Silicon on yield quality of rice plant grown under drought Stress. Australian Journal of Crop Science, 8(4), 596-605.

Feng, W., Lindner, H., Robbins, N. E., & Dinneny, J. R. (2016). Growing out of Stress: The role of cell- and organ-scale growth control in plant water-stress responses. The Plant Cell, 28(8), 1769-1782.

Giannopolitis, C. N., & Ries, S. K. (1977). Superoxide dismutase: I. occurrence in higher plants. Plant Physiology, 59(2), 309-314.

Hadwiger, L. A. (2013). Multiple effects of chitosan on plant systems: Solid Science or hype. Plant Science, 208(10.1016), 42-49. DOI: 10.1016/J.PLANTSCI.2013.03.007

Havir, E. A., & Mchale, N. A. (1987). Biochemical and developmental characterization of multiple forms of catalase in tabacco leaves. Plant Physiology, 84(2), 450-455.

Iriti, M., Picchi, V., Rossoni, M., Gomarasca, S., Ludwig, N., Gargano, M., & Faoro, F. (2009). Chitosan antitranspirant activity is due to abscisic acid-dependent stomatal closure. Envionmental and Experimental Botany, 66(2), 493-500, 2009. DOI: 10.1016/j.envexpbot.2009.01.004

Katiyar, D., Hemantaranjan, A., & Singh, B. (2015). Chitosan as a promising natural compound to enhance potential physiological responses in plant: a review. Indian Journal of Plant Physiology, 20(1), 1-9. DOI: 10.1007/s40502-015-0139-6

Magalhães, P. C., & Durães, F. O. M. (2008). Fisiologia da produção. A cultura do milho. Sete Lagoas, MG: Embrapa Milho e Sorgo.

Nakano, Y., & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbato specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22(5), 867-880.

Pichyangkura, R., & Chadchawan, S. (2015). Biostimulant activity of chitosan in horticulture. Scientia Horticulturae, 196(30), 49-65. DOI: 10.1016/j.scienta.2015.09.031

Pirbalouti, A. G., Malekpoor, F., Salimi, A., & Golparvar, A. (2017). Exogenous application of chitosan on biochemical and physiological characteristics, phenolic contente and antioxidante activity of two species of basil (Ocimumciliatum and Ocimumbasilicum) under reduced irrigation. Scientia Horticulturae, 201(15), 114-122, 2017. DOI: 10.1016/j.scienta.2017.01.031

Rizzi, V., Fini, P., Semeraro, P., & Cosna, P. (2016). Detailed investigation of ROS arisen from chlorophyll a/Chitosan based-biofilm. Colloids Surfaces B-Biointerfaces, 142(1), 239-247. DOI: 10.1016/j.colsurfb.2016.02.062

Scholander, P. F., Hammel, H. T., Hemingsen, E. A., & Bradstreet, E. D. (1964). Hydrostatic pressure and osmotic potentials in leaves of mangroves and some other plants. Proceedings of National Academy of Science, 51(4), 119-125.

Silveira, J. A. G., Araújo, S. A. M., Lima, J. P. M. S., & Viégas, R. A. (2009). Roots and leaves display contrasting osmotic adjustment mechanisms in response to NaCl-salinity in Atriplexnumularia. Enveronmental and Experimental Botany, 66(1) 1-8. DOI: 10.1016/j.envexpbot.2008.12.015

Souza, T. C., Magalhães, P. C., Castro. E. M., Carneiro, N. P., Padilha, F. A., & Júnior, C. C. G. (2014). ABA application to maize hybrids contrasting for drought tolerance: changes in water parameters and in antioxidant enzyme activity. Plant Growth Regulation, 73(3), 205-217. DOI: 10.1007/s10725-013-9881-9

Velikova, V., Yordanov, I., & Edreva, A. (2000). Oxidative Stress and some antioxidant Systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Science, 151(1), 59-66. DOI: 10.1016/S0168-9452(99)00197-1

Zucker, M. (1965). Induction of phenylalanine deaminase by ligth and its relation to chlorogenic acid synthesis in potato tuber tissue. Plant Physiology, 40(5), 779-784.