Bacillus amyloliquefaciens PKM16 acts as an antagonist of white mold and an inducer of defense enzymes in tomato plants

Amanda do Prado Mattos; Bruna Broti  Rissato; Adriana Terumi  Itako; Joao Batista  Tolentino Junior; Kátia Regina Freitas Schwan  Estrada

doi:10.4025/actasciagron.v45i1.59586

Amanda do Prado Mattos Universidade Estadual de Maringá https://orcid.org/0000-0002-6855-4056
Bruna Broti Rissato Universidade Estadual de Maringá https://orcid.org/0000-0001-6197-8254
Adriana Terumi Itako Universidade Federal de Santa Catarina https://orcid.org/0000-0002-1024-2658
Joao Batista Tolentino Junior Universidade Federal de Santa Catarina https://orcid.org/0000-0003-1303-4202
Kátia Regina Freitas Schwan Estrada Universidade Estadual de Maringá https://orcid.org/0000-0001-8384-8557

DOI: https://doi.org/10.4025/actasciagron.v45i1.59586

Keywords: biological control; defense mechanisms; micro-tom; pathogenesis-related proteins; rhizobacteria; Sclerotinia sclerotiorum.

Abstract

This study aimed to investigate the potential of rhizobacteria isolated from tomato plants to control Sclerotinia sclerotiorum and induce the activity of pathogenesis-related enzymes in Micro-Tom tomato plants. Three rhizobacterial isolates were evaluated to determine the most efficient antagonist agent, which was later identified by gene sequencing as Bacillus amyloliquefaciens PKM16. The antagonistic effects of B. amyloliquefaciens against S. sclerotiorum were assessed in vivo and in vitro using live and autoclaved cultures at concentrations of 0% (control), 20%, 30%, and 40% (v/v). The residual effects of four treatments (20% live culture, 20% autoclaved culture, a Bacillus subtilis-based commercial product, and autoclaved distilled water) on tomato plants inoculated with S. sclerotiorum were determined. The same treatments were also used to assess the myceliogenic germination of sclerotia and induction of plant defense enzymes (peroxidase, catalase, polyphenol oxidase, phenylalanine ammonia-lyase, and β-1,3-glucanase) in tomato plants. The live culture had a residual effect for 4 days and inhibited sclerotial germination by approximately 30%. Furthermore, live and autoclaved bacterial growth cultures stimulated enzyme activity. Therefore, B. amyloliquefaciens PKM16 was antagonistic to S. sclerotiorum, effectively inhibiting mycelial growth and activating defense mechanisms in Micro-Tom tomato plants.

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Bacillus amyloliquefaciens PKM16 acts as an antagonist of white mold and an inducer of defense enzymes in tomato plants

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