<b>Micropropagation of an <em>Eucalyptus</em> hybrid (<em>Eucalyptus benthamii x Eucalyptus dunnii</em>)</b> - doi: 10.4025/actasciagron.v33i4.8317

  • Gilvano Ebling Brondani Universidade Federal do Paraná
  • Leonardo Ferreira Dutra Empresa Brasileira de Pesquisa Agropecuária
  • Ivar Wendling Empresa Brasileira de Pesquisa Agropecuária
  • Fernando Grossi Universidade Federal do Paraná
  • Fabricio Augusto Hansel Empresa Brasileira de Pesquisa Agropecuária
  • Marla Alessandra Araujo Universidade Federal de Lavras
Keywords: in vitro establishment, culture medium, cloning, BAP, NAA, GA3

Abstract

This study was designed to micropropagate E. benthamii x E. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) for bud proliferation, and the ratio between BAP and gibberellic acid (GA3) in two nutrient media for shoot elongation. Nodal segments from H12, H19 and H20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v-1) of chlorine. Explants were grown on ½MS medium supplemented with BAP (0, 0.25, 0.50, 0.75 and 1.00 mg L-1) and NAA (0, 0.025, 0.050, 0.075 and 0.100 mg L-1) for bud production. They were elongated on MS and ½MS media supplemented with BAP (0, 0.05 and 0.10 mg L-1) and GA3 (0, 0.1, 0.2 and 0.3 mg L-1). The 0.50 mg L-1 BAP and 0.050 mg L-1 NAA combination was optimal for bud proliferation for H12 and H20. GA3 concentrations of 0.10 and 0.2-1 mg L-1 combined with 0.10 mg L-1 BAP on ½MS resulted in the longest shoots, for H12 and H20, respectively. Regardless of clone, the rooting rate was low, with an average of 12.0% and 14.4% of plants having roots for in vitro and ex vitro conditions, respectively.

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Author Biographies

Gilvano Ebling Brondani, Universidade Federal do Paraná
strando em Engenharia Florestal http://lattes.cnpq.br/4095993222888432
Leonardo Ferreira Dutra, Empresa Brasileira de Pesquisa Agropecuária
Eng. Agrônomo Dr. em Agronomia
Ivar Wendling, Empresa Brasileira de Pesquisa Agropecuária
Eng. Florestal Dr. em Ciências Florestais
Fernando Grossi, Universidade Federal do Paraná
Eng. Florestal Dr. em Ciências Florestais
Fabricio Augusto Hansel, Empresa Brasileira de Pesquisa Agropecuária
Químico
Marla Alessandra Araujo, Universidade Federal de Lavras
Eng. Agronoma (UFPR), Mestre em Ciência do Solo (UFPR), Doutoranda em Ciência do Solo (UFLA)
Published
2011-05-04
How to Cite
Brondani, G. E., Dutra, L. F., Wendling, I., Grossi, F., Hansel, F. A., & Araujo, M. A. (2011). <b>Micropropagation of an <em>Eucalyptus</em> hybrid (<em>Eucalyptus benthamii x Eucalyptus dunnii</em>)</b&gt; - doi: 10.4025/actasciagron.v33i4.8317. Acta Scientiarum. Agronomy, 33(4), 655-663. https://doi.org/10.4025/actasciagron.v33i4.8317
Section
Crop Production

 

2.0
2019CiteScore
 
 
60th percentile
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2.0
2019CiteScore
 
 
60th percentile
Powered by  Scopus