<b>In vitro multiplication of rootstock grapevine in variations of culture media</b> - DOI: 10.4025/actasciagron.v28i3.954

Abstract

The grapevine micropropagation can generate virus-free plants and in short time period. Aiming at improving in vitro propagation techniques, concentrations of MS culture medium and sucrose were tested. Axillary buds (2.0 cm length) of ‘043-43’ rootstocks maintained in vitro were excisaded in aseptic conditions and inoculated in tubes with five concentrations of MS culture medium (0, 50, 100, 150 and 200%) and five different sucrose concentrations (0, 15, 30, 45 and 60 g L^-1), in all possible combinations. The pH was adjusted for 5.8 before the addition of 6.0 g L^-1 agar and sterilization to 121ºC and 1 atm for 20 minutes. After inoculation, the explants were maintained for 60 days, in growth room with temperature of 25 ± 1ºC, irradiance of 32 mol.m^-2.s^-1 and photoperiod of 16 hours. The experiment was arranged in a completely randomized design, in a 5x5 factorial scheme, using four repetitions with 12 plants per treatment. The numbers of leaves, number of roots per plant, length of the aerial part and fresh weigh of the aerial part were evaluated. There is influence of the concentration of the culture medium and the sucrose on the growth and development of the aerial part and the roots of grapevine ‘043-43’ rootstock. For the grapevine rootstock micropropagation, better results are obtained with the MS culture medium in its original concentration, supplemented with 15- 30 g L^-1 of sucrose.