<b><i>In vitro</i> cultivation of zygotic embryos from Murici (<i>Byrsonima cydoniifolia</i> A. Juss.): establishment, disinfection, and germination</b> - doi: 10.4025/actasciagron.v35i2.15402

  • Cíntia de Oliveira Martendal Universidade Estadual de Maringá
  • Murilo Martins Bernardino Instituto Federal Goiano
  • Flávia Dionísio Pereira Cooperativa Agroindustrial dos Produtores Rurais do Sudoeste Goiano Ltda.
  • Fabiano Guimarães Silva Instituto Federal Goiano
  • Carlos César Evangelista de Menezes Cooperativa Agroindustrial dos Produtores Rurais do Sudoeste Goiano Ltda.
  • Alessandra Cristina Boffino de Almeida Monteiro Hara Instituto Federal Goiano

Resumo

The objective of this study is to establish an in vitro germination and cultivation protocol for murici (Byrsonima cydoniifolia A. Juss.) using zygotic embryos. Therefore, three assays were performed: in assay I, embryo asepsis was tested at exposure times of 5, 10, 15, 20, 25, and 30 minutes in 2.5% sodium hypochlorite, with or without immersion in 70% alcohol; in assay II, MS (MURASHIGE; SKOOG, 1962) e WPM (LLOYD; McCOWN, 1980) culture media were tested at salt concentrations of 25, 50, and 100%, with or without the addition of sucrose, to germinate the buds; in assay III, seedling growth was evaluated in MS and WPM culture media at salt concentrations of 25, 50 and 100%. Sodium hypochlorite (2.5%) with or without 70% alcohol was used to avoid contamination because it was not toxic to murici embryos. Water-agar was the most appropriate culture medium for bud germination, and 50% WPM was appropriate for seedling growth.

 

 

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Publicado
2012-12-20
Como Citar
Martendal, C. de O., Bernardino, M. M., Pereira, F. D., Silva, F. G., de Menezes, C. C. E., & Monteiro Hara, A. C. B. de A. (2012). <b><i>In vitro</i> cultivation of zygotic embryos from Murici (<i>Byrsonima cydoniifolia</i> A. Juss.): establishment, disinfection, and germination</b&gt; - doi: 10.4025/actasciagron.v35i2.15402. Acta Scientiarum. Agronomy, 35(2), 221-229. https://doi.org/10.4025/actasciagron.v35i2.15402
Seção
Produção Vegetal

 

2.0
2019CiteScore
 
 
60th percentile
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2.0
2019CiteScore
 
 
60th percentile
Powered by  Scopus