Effect of LCORL gene polymorphism on body size traits in horse populations
Resumo
The aim of this study was to determine polymorphism of LCORL gene in horse breeds and its association with body size. PCR-RFLP technique was performed using AluI for genotyping of 306 horses. Results showed that C is the rare allele in Iranian Breeds, because these horses have been used since ancient times as a courier and for war and archery, hence selection has done to benefit of spiky horses with medium body that need less food and are tireless. While, for foreign breeds; frequency of C allele was high that can be concluded these breeds used in fields, forests, and mines. A UPGMA dendrogram based on the Nei's standard genetic distance among studied breeds showed separate clusters for Iranian native and exotic breeds. Statistical association analysis of three observed genotypes with body size showed that there is an association between this polymorphism and body size criteria (p < 0.01). Overall, it can be concluded that studied mutation in LCORL gene can be used as candidate marker for improving body weight in horse.
Downloads
Referências
Boyko, A. R., Brooks, S. A., Behan-Braman, A., Castelhano, M., Corey, E., Oliveira, K. C., ... Ainsworth, D. M. (2014). Genomic analysis establishes correlation between growth and laryngeal neuropathy in Thoroughbreds. BMC genomics, 15(1), e259. doi: 10.1186/1471-2164-15-259
Carty, C. L., Johnson, N. A., Hutter, C. M., Reiner, A. P., Peters, U., Tang, H., & Kooperberg, C. (2012). Genome-wide association study of body height in African Americans: The women's health initiative SNP health association resource (share). Human Molecular Genetics, 21(3), 711-720. doi: 10.1093/hmg/ddr489
Dorman, K., Shen, Z., Yang, C., Ezzat, S., & Asa, S. L. (2012). CtBP1 interacts with Ikaros and modulates pituitary tumor cell survival and response to hypoxia. Molecular Endocrinology, 26(3), 447-457. doi: 10.1210/me.2011-1095
Ebrahimi, M. T. V., Mohammadabadi, M., & Esmailizadeh, A. (2017). Using microsatellite markers to analyze genetic diversity in 14 sheep types in Iran. Archives Animal Breeding, 60(3), 183-189. doi: doi:10.5194/aab-60-183-2017
Fotovati, A. (2000). Persian horse breeds from ancient time to present and their rules in development of world horse breeds. Asian-Australas Journal of Animal Science, 13, 401-401.
Han, Y. J., Chen, Y., Liu, Y., & Liu, X. L. (2017). Sequence variants of the LCORL gene and its association with growth and carcass traits in Qinchuan cattle in China. Journal of Genetics, 96(1), 9-17.
He, S., Zhang, L., Li, W., & Liu, M. (2015). BIEC2-808543 SNP in the LCORL gene is associated with body conformation in the Yili horse. Animal biotechnology, 26(4), 289-291. doi: 10.1080/10495398.2014.995303
Kajimura, S., Seale, P., Tomaru, T., Erdjument-Bromage, H., Cooper, M. P., Ruas, J. L., ... Spiegelman, B. M. (2008). Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex. Genes & Development, 22(10), 1397-1409. doi: 10.1101/gad.1666108
Khodabakhshzadeh, R., Mohammadabadi, M. R., Esmailizadeh, A. K., Shahrebabak, H. M., Bordbar, F., & Namin, S. A. (2016). Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep. Polish Journal of Veterinary Sciences, 19(2), 281-289. doi: 10.1515/pjvs-2016-0035
Kim, W., Bennett, E. J., Huttlin, E. L., Guo, A., Li, J., Possemato, A., ... Comb, M. J. (2011). Systematic and quantitative assessment of the ubiquitin-modified proteome. Molecular cell, 44(2), 325-340. doi: 10.1016/j.molcel.2011.08.025
Kimura, Y., & Tanaka, K. (2010). Regulatory mechanisms involved in the control of ubiquitin homeostasis. The journal of biochemistry, 147(6), 793-798. doi: 10.1093/jb/mvq044
Liu, R., Sun, Y., Zhao, G., Wang, H., Zheng, M., Li, P., ... Wen, J. (2015). Identification of loci and genes for growth related traits from a genome-wide association study in a slow-× fast-growing broiler chicken cross. Genes & Genomics, 37(10), 829-836. doi: 10.1007/s13258-015-0314-1
Makvandi-Nejad, S., Hoffman, G. E., Allen, J. J., Chu, E., Gu, E., Chandler, A. M., ... Brooks, S. A. (2012). Four loci explain 83% of size variation in the horse. PloS One, 7, e39929. doi: 10.1371/journal.pone.0039929
McNally, K., Cotton, R., Hogg, A., & Loizou, G. (2014). PopGen: a virtual human population generator. Toxicology, 315, 70-85. doi: 10.1016/j.tox.2013.07.009
Metzger, J., Schrimpf, R., Philipp, U., & Distl, O. (2013). Expression levels of LCORL are associated with body size in horses. PloS One, 8(2), e56497. doi: 10.1371/journal.pone.0056497
Mohammadabadi, M. R., Nikbakhti, M., Mirzaee, H. R., Shandi, A., Saghi, D. A., Romanov, M. N., & Moiseyeva, I. G. (2010). Genetic variability in three native Iranian chicken populations of the Khorasan province based on microsatellite markers. Russian Journal of Genetics, 46(4), 505-509.
Mousavizadeh, A., Mohammad Abadi, M., Torabi, A., Nassiry, M. R., Ghiasi, H., & Koshkoieh, A. A. (2009). Genetic polymorphism at the growth hormone locus in Iranian Talli goats by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Iranian Journal of Biotechnology, 7(1), 51-53.
Ruzina, M. N., Shtyfurko, T. A., Mohammadabadi, M. R., Gendzhieva, O. B., Tsedev, T., & Sulimova, G. E. (2010). Polymorphism of the BoLA-DRB3 gene in the Mongolian, Kalmyk, and Yakut cattle breeds. Russian Journal of Genetics, 46(4), 456-463.
Shamsalddini, S., Mohammadabadi, M. R., & Esmailizadeh, A. K. (2016). Polymorphism of the prolactin gene and its effect on fiber traits in goat. Russian Journal of Genetics, 52(4), 405-408.
Signer-Hasler, H., Flury, C., Haase, B., Burger, D., Simianer, H., Leeb, T., & Rieder, S. (2012). A genome-wide association study reveals loci influencing height and other conformation traits in horses. PloS One, 7(5), e37282. doi: 10.1371/journal.pone.0037282
Tetens, J., Widmann, P., Kühn, C., & Thaller, G. (2013). A genome‐wide association study indicates LCORL/NCAPG as a candidate locus for withers height in G erman W armblood horses. Animal Genetics, 44(4), 467-471. doi: 10.1111/age.12031
Vinayagam, A., Stelzl, U., Foulle, R., Plassmann, S., Zenkner, M., Timm, J., ... Wanker, E. E. (2011). A directed protein interaction network for investigating intracellular signal transduction. Science Signal, 4(189), rs8-rs8. doi: 10.1126/scisignal.2001699
Xiong, Y., Wang, E., Huang, Y., Guo, X., Yu, Y., Du, Q., … Sun, Y. (2016). Inhibition of lysine-specific demethylase-1 (LSD1/KDM1A) promotes the adipogenic differentiation of hESCs through H3K4 methylation. Stem Cell Reviews and Reports, 12(3), 298-304. doi: 10.1007/s12015-016-9650-z
Zamani, P., Akhondi, M., & Mohammadabadi, M. (2015). Associations of inter-simple sequence repeat loci with predicted breeding values of body weight in sheep. Small Ruminant Research, 132, 123-127. doi: 10.1016/j.smallrumres.2015.10.018
DECLARAÇÃO DE ORIGINALIDADE E DIREITOS AUTORAIS
Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade.
Os direitos autorais pertencem exclusivamente aos autores. Os direitos de licenciamento utilizados pelo periódico é a licença Creative Commons Attribution 4.0 (CC BY 4.0): são permitidos o compartilhamento (cópia e distribuição do material em qualqer meio ou formato) e adaptação (remix, transformação e criação de material a partir do conteúdo assim licenciado para quaisquer fins, inclusive comerciais.
Recomenda-se a leitura desse link para maiores informações sobre o tema: fornecimento de créditos e referências de forma correta, entre outros detalhes cruciais para uso adequado do material licenciado.
