<b>Ovary morphology of buffalo heifers fattened in feed lot in response of the utilization of growth promoter or leads sphere introduced in the uterus</b> - DOI: 10.4025/actascianimsci.v26i2.1870

Abstract

The objective of this work was to evaluate the hormonal implant use (Synovex-H^® - 20mg of benzoate of estradiol and 200mg of testosterone) and 100 lead spheres in the uterus on the ovary activity of buffalo heifers in feed lot. Twenty buffalo heifers of breed Murrah were used, with live weight of 350kg and 16 months of age, distributed at random in three treatments: empty heifers (EMH), heifers with growth promoter (GPR) and heifers with lead sphere (LEA) introduced in the uterus. The heifers were weighed each 28 days. At the end of the experiment, the heifers were slaughtered and the reproductive system was collected. The uterus was analyzed microscopically. The ovaries were longitudinally cut and fixed in Bouin liquid for 24 hours to microscopic analyses. After, the ovaries were included in paraffin and cut with 5μm of thickness and submitted to the Hematoxilin-Eosin color technique for identification of follicular development. In the treatments EMH and LEA the uterus microscopic analyses did not present differences between themselves, presenting normal aspect. In the treatment GPR, it was observed thicker muscles, bigger size and darker color. In addition, the lead spheres did not interfere in the ovary activity. This must has happened because the lead spheres were not kept in the interior of the uterus, with exception of one animal that presented lead spheres. In this case, the uterus presents an aspect inflated. In the microscopic observations, ovary activity was consisted of three treatments.