<b>Comparison of DNA extraction protocols in <em>Lernaea</em> sp. (Copepoda: Cyclopoida)</b>- DOI: 10.4025/actascianimsci.v25i2.1973

  • Marivone Valentim UFP
  • Lauro Vargas UEM
  • Héden Marques Moreira UEM
  • Ricardo Pereira Ribeiro UEM
DOI: https://doi.org/10.4025/actascianimsci.v25i2.1973

Abstract

In this study thirty samples of adult females of Lernaea sp. were collected from naturally infested fishes at two fisheries in northern Paraná, Brazil. Two protocols of DNA extraction were tested for parasites tissues. The results have shown that the protocol using Kit Puregene DNA Isolation (GENTRA) was adequate for genomic DNA extraction in Lernaea. The methodology using CTAB protocol, described by Black IV et al. (1996), modified for DNA extraction in Lernaea sp., did not work properly.

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Author Biography

Marivone Valentim, UFP
Possui graduação em Medicina Veterinária pela Universidade Federal do Paraná (1989). Atualmente é Professor Adjunto da Universidade Federal do Paraná-Campus Palotina. Tem experiência na área de Medicina Veterinária, com ênfase em Parasitologia Animal, pesquisando principalmente nos seguintes temas: Parasitologia em peixes, Biologia Molecular de parasitos e Homeopatia populacional Currículo Lattes
Published
2008-04-16
How to Cite
Valentim, M., Vargas, L., Moreira, H. M., & Ribeiro, R. P. (2008). <b>Comparison of DNA extraction protocols in <em>Lernaea</em> sp. (Copepoda: Cyclopoida)</b&gt;- DOI: 10.4025/actascianimsci.v25i2.1973. Acta Scientiarum. Animal Sciences, 25(2), 219-222. https://doi.org/10.4025/actascianimsci.v25i2.1973
Issue
Vol 25 No 2 (2003)
Section
Animal Science

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