<b>Super-ovulation in rabbit does fed on rations containing different sources of vegetable oil</b> - DOI: 10.4025/actascianimsci.v28i3.45

Abstract

Effects of super-ovulation on 40 rabbit does were evaluated. Females were distributed in a completely randomized design, with four treatments (oil-free ration and rations with 3% canola oil, corn oil or soybean oil) and 10 replications. Each doe received a unique intramuscular dose of 40 UI of equine corionic gonadotrophin (eCG); 48 hours later they underwent breeding. After 72 hours of breeding, the does were slaughtered and 10mL blood samples were collected for progesterone and 17β-estradiol serum level analyses. Other parameters, such as weight of uterus with ovaries, number of follicles and of corpora lutea, were obtained. Embryos were recovered by three flushings, per uterine corn, with Dulbecco extender (PBS). The recovered effluent was analyzed to find out structures and classify them according to morphology and age. No differences were observed (p>0.05) in progesterone and 17β-estradiol serum levels, uterus plus ovaries weight, number of follicles and of corpora lutea in the right and left ovaries, number of viable and degenerated structures. Treatment affected (p<0.05) embryo production: it was lower in rabbit does fed on 3% canola oil rations and higher in those fed on vegetable oilfree rations