Short communication: psychrotrophic microorganism count in raw milk samples preserved with azidiol®
Abstract
The study aimed to evaluate the use of the azidiol® preservative for psychrotrophic microorganism count (PMC) in cooled raw milk. Two studies were carried out, one under controlled conditions (experiment 1) and the other under field conditions (experiment 2), in which samples of raw milk were taken with and without the use of the azidiol® preservative and analyzed at predefined times (0, 6, 12 and 24 hours - experiment 1) and at varying times (experiment 2). Analysis of variance and regression analysis using SAS were applied for data statistical analysis. Milk samples without azidiol® showed higher PMC with increasing time between sampling and analysis, while in samples preserved with azidiol®, this count remained constant. Samples of cooled raw milk intended for PMC should be collected in flasks containing the azidiol® preservative.
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References
Arcuri, E. F., Brito, M. A. V. P., Brito, J. R. F., Pinto, S. M., Ângelo, F. F., & Souza, G. N. (2008). Qualidade microbiológica do leite refrigerado nas fazendas. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 58(3): 440-446. DOI: https://doi.org/10.1590/S0102-09352006000300024
Cassoli, L. D., Machado, P. F., & Coldebella, A. (2010). Métodos de conservação de amostras de leite para determinação da contagem bacteriana total por citometria de fluxo. Revista Brasileira de Zootecnia, 39(2), 434-439. DOI: https://doi.org/10.1590/S1516-35982010000200029
Fagnani, R., Schuck, J., Botaro, B. G., & Santos, F. C. (2017). Extended storage of cold raw milk on yogurt manufacturing. Pesquisa Agropecuária Brasileira, 52(2), 104-112. DOI: https://doi.org/10.1590/S0100-204X2017000200004
Frank, J. F. & Yousef, A. E. (2004). Tests for groups of microorganisms. In H. M. Wehr & J. F. Frank (ed.), Standard methods for the examination of dairy products (17th ed, p. 242-245). Washington, DC: American Public Health Association.
Garnica, M. L., Santos, J. A., & Gonzalo, C. (2011). Short communication: Influence of storage and preservationon microbiological quality of silo ovine milk. Journal of Dairy Science, 94(4), 1922-1927. DOI: https://doi.org/10.3168/jds.2010-3787
Izidoro, T. B., Pereira, J. G., Soares, V. M., Spina, T. L. B., & Pinto, J. P. A. N. (2013). Atividade proteolítica de bactérias psicrotróficas em leites estocados em diferentes temperaturas. Revista Ceres, 60(4): 452-457. DOI: https://doi.org/10.1590/S0034-737X2013000400002
Marioto, L. R. M. M., Daniel, G. C., Gonzaga, N., Mareze, J., Tamanini, R., & Beloti, V. (2020). Potencial deteriorante da microbiota mesófila, psicrotrófica, termodúrica e esporulada do leite cru. Ciência Animal Brasileira, 21, e-44034. DOI: https://doi.org/10.1590/1809-6891v21e-44034
Nörnberg, M. F. B. L., Tondo, E. C., & Brandelli, A. (2009). Bactérias psicrotróficas e atividade proteolítica no leite cru refrigerado. Acta Scientiae Veterinariae, 37(2), 157-163. DOI: https://doi.org/10.22456/1679-9216.16243
Paludetti, L. F., Kelly, A. L., & Gleeso, D. (2020). Effect of thermoresistant protease of Pseudomonas fluorescens on rennet coagulation properties and proteolysis of milk. Journal of Dairy Science, 103(5), 4043-4055. DOI: https://doi.org/10.3168/jds.2019-17771
Reche, N. L. M., Thaler Neto, A., D’Ovidio, L., Felipus, N. C., Pereira, L. C., Cardozo, L. L., ... Picinin, L. C. A. (2015). Multiplicação microbiana no leite cru armazenado em tanques de expansão direta. Ciência Rural, 45(5), 828-834. DOI: https://doi.org/10.1590/0103-8478cr20140542
Santana, E. H. W., Beloti, V., Barros, M. A. F., Moraes, L. B., Gusmão, V. V., & Pereira, M. S. (2001). Contaminação do leite em diferentes pontos do processo de produção: I. Microrganismos aeróbios mesófilos e psicrotróficos. Semina: Agrárias, 22(2), 145-154. DOI: https://doi.org/10.5433/1679-0359.2001v22n2p145
Santos, M. C. M., Cerqueira, M. M. O. P., Leite, M. O., & Souza, M. R. (2018). Desenvolvimento de um modelo preditivo para identificação de perda de estabilidade e ocorrência de proteólise em leite UAT. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 70(1), 247-253. DOI: https://doi.org/10.1590/1678-4162-9256
Statistical Analysis Sofware [SAS]. (2002). User guide. Version 8 [CD-ROM]. Cary, NC: SAS Insitute Inc.
Vithanage, N. R., Dissanayake, M., Bolge, G., Palombo, E. A., Yeager, T. R., & Datta, N. (2017). Microbiological quality of raw milk attributable to prolonged refrigeration conditions. Journal of Dairy Research, 84, 92-101. DOI: https://doi.org/10.1017/S0022029916000728
Wentz, A. G., Bermudes, R. F, Martins, C. M. M., Osmari, M. P., Rodrigues, B. P., & Pozza, M. S. S. (2018). Different methods and times of milk conservation: physical-chemical composition and microbiological quality. Acta Veterinaria Brasilica, 12, 84-93. DOI: http://dx.doi.org/10.21708/avb.2018.12.3.7698
Zhang, S., Li, H., Uluko, H., Liu, L., Pang, X., & Jiaping, L. V. (2015). Investigation of protease production by Pseudomonas fluorescens bj-10 and degradation on milk proteins. Journal of Food Processing and Preservation, 39(6), 2466-2472. DOI: https://doi.org/10.1111/jfpp.12496
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