Performance and Welfare of Laying Hens Raised on Floor in Relation to Different Housing Densities
Abstract
This study analyzed the productive performance, egg quality and physiological and behavioral parameters of laying hens at different housing densities in a cage-free system, following animal welfare guidelines and improvements in animal housing in intensive systems. 252 Novogen Brown laying hens, 52 weeks old and with an average weight of 1,740 ± 0.11 kg, were used over 63 days, divided into three periods of 21 days. The study adopted a completely randomized experimental design, with four treatments and seven replications. The housing densities tested were: 6 birds box-1 (0.406 m2 bird-1), 8 birds box-1 (0.305 m2 bird-1), 10 birds box-1 (0.244 m2 bird-1) and 12 birds box-1 (0.203 m2 bird-1 bird). The data were analyzed for normality of residuals, homogeneity of variance and independence of errors, using linear and quadratic regression models for each variable. In cage-free poultry production systems, housing densities of 6 to 8 birds per box can be used without affecting performance, egg quality, physiological or behavioral parameters of the hens.
Downloads
References
Aguiar, D. P., Valentim J. K., Lima, H. J. Á., Bittencourt, T. M., Andreoti, L. Z., Pereira, I. D. B., & Zanella, J. (2021). Beak trimming and stocking densities for laying and performance traits and behavioral patterns in Japanese quails. Revista de Investigaciones Veterinarias del Perú, 32(5), 1-9. http://dx.doi.org/10.15381/rivep.v32i5.19248
Andrade, R. R., Tinôco, I. F. F., Souza, C. F., Oliveira, K. P. M., Barbari, V; Cruz, M. F., Baptista, F. J. F., Vilela, M. O., Conti, L., & Rossi, G. (2018). Effect of thermal environment on body temperature of systems for laying hens. Agronomy Research, 16(2), 320-327. https://doi.org/10.15159/AR.18.068
Attia, Y. A., Abou-Shehema, B. M., Abdellah, A. A., Aly, O. M., & El-Naggar, A. S. (2020). Effect of ascorbic acid and/or alpha-tocopherol fortification on semen quality, metabolic profile, antioxidants status, and DNA of roosters exposed to heat stress. Journal of Animal and Plant Sciences, 30(2), 325-335. https://doi.org/10.36899/japs.2020.2.0051
Barros, A. F., & Souza Junior, R. A. (2021). Física e biomecânica de animais. Caderno Intersaberes, 10(27), 4-24.
Bittencourt, T. M., Lima, H. J. D., Amorim, C. M. M., Cruz, M. S., Morais, M. V. M., & Pinto, V. M. (2023). Desempenho termorregulação e viabilidade econômica de galinhas alimentadas com grãos secos de destilaria de milho na fase de pré-postura. Revista Acadêmica Ciência Animal, 21(1), 1-10. http://dx.doi.org/10.7213/acad.2023.21002
Dias, A. N., Maciel, M. P., Oliveira Aiura, A. L., Arouca, C. L. C., Silva, D. B., & Moura, V. H. S. (2016). Pesquisa Agropecuária Brasileira, 51(12), 2010-2017. https://doi.org/10.1590/S1678-3921.pab2016.v51.22793
Eisen, E. J., Bohren, B. B., & McKen, H. E. (1962). The haung unit as a mensure of eggs albumen quality. Poultry Science, 41(5), 1461-1468. https://doi.org/10.3382/ps.0411461
Farinhas, M. V. S., Quintero, J. C. P., Moreira, Y. R., Rocha, S. S., Reis, T. L., & Curvello, R. A. (2023). Diferentes densidades de alojamento de galinhas poedeiras em gaiolas e suas respectivas produção, qualidade de ovos e escore de penas. Revista de Ciências Agroveterinárias, 22(3), 463-469. http://dx.doi.org/10.5965/223811712232023463
Grunitzky, L., Centenaro, J. R., Silva, N. R., Paulo, J. M., Silveira, A. M., Lopes, G. V., Felix, G. A., & Braz, P. H. (2020). Thermal stress alters the basal value of serum proteins in laying hens. Revista Brasileira de Saúde e Produção Animal, 21(1), 1-10. http://dx.doi.org/10.1590/S1519-99402121062020
Kang, H. K., Park, S. B., Jeon, J. J.,Skim, H. S., Kim, S. H., Hong, E., & Kim, C. H. (2018). Effect of stocking density on laying performance, egg quality and blood parameters of Hy-Line Brown laying hens in an aviary system. European Poultry Science, 82(1), 1-13. http://dx.doi.org/10.1399/eps.2018.245
Kim, D. H., Lee, Y. K., Lee, S. D., Kim, S. H., & Lee, K. W. (2021). Physiological and behavioral responses of laying hens exposed to long-term high temperature. Journal of Thermal Biology, 99(1), 1-7. https://doi.org/10.1016/j.jtherbio.2021.103017
Kunzler, B. E., Pimentel, L. R., & Souza, J. D. P. (2023). Avaliação da qualidade de ovos comercializados no município de Dianópolis, estado do Tocantins. Revista Sítio Novo, 7(4), 85-97.
Moura, A. C., Nascimento, G. Miranda, M. S., J. L., Martins, K. D. N., Santo, L. F., & Pompeu, M. A. (2022). Desenvolvimento de material informativo sobre criação de aves no sistema cage-free. Revista Sinapse Múltipla, 11(1), 140-142.
Nawab, A., Tang, S., Li, G., An, L., Wu, J., Liu, W., & Xiao, M. (2020). Dietary curcumin supplementation effects on blood immunological profile and liver enzymatic activity of laying hens after exposure to high temperature conditions. Journal of Thermal Biology, 90(1), 1-15. https://doi.org/10.1016/j.jtherbio.2020.102573
Netto, D. A., Lima, H. J. D., Alves, J. R. B. C., Morais, M. V.M., Rosa, M. S., & Bittencourt, T. M. (2018). Production of laying hens in different rearing systems under hot weather. Acta Scientiae, 40(1), 2-6. https://doi.org/10.4025/actascianimsci.v40i1.37677
Netto, D. A., Procópio, D. P., & Lima, H. J. D. (2023). Risco e desempenho econômico de produção de galinhas poedeiras em diferentes sistemas de criação sob a condição de clima quente. Extensão Rural, 30(1), 1-19. https://doi.org/10.5902/2318179663572
Paiva, L. L., Nascimento, K. M. R. S., Silva, F. N. S., Freitas, H. B., Silva, H. B., T. R., Ofico, A. V., Chaves, L. A., Silva, L. R. A., Macie, V. A., & Santos, C. B. T. (2019). Qualidade de ovos brancos comerciais em diferentes temperaturas de conservação e período de estocagem. Periódicos Brasileiros em Medicina Veterinária e Zootecnia, 76(1), 1-8. https://doi.org/10.17523/bia.2019.v76.e1457
Philippe, F. X., Mahmoudi, Y., Cinq-Mars, D., Lefrançois, M., Moula Palacios, M., J., Pelletier, F., & Godbout, S. (2020). Comparison of egg production, quality and composition in three production early-stage laying hens. Livestock Science, 232(1), 1-10. https://doi.org/10.1016/j.livsci.2020.103917
R Core Team (2018). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://r-project.org
Reis, T. L., Quintero, J. C. P., Luchese, R. H., Adler, G. H., Freitas Junior, C. V., Silva, L. G., & Calixto, L. F. L. (2019). Influência do sistema de criação em piso sobre cama e gaiola sobre as características ósseas e a qualidade físico-química e microbiológica de ovos de galinhas Arquivos Brasileiros de Medicina Veterinária e Zootecnia, 71(5), 1623-1630. http://dx.doi.org/10.1590/1678-4162-11043
Richards, S. A. (1971). The significance of changes in the temperature of the skin and body core of the chicken in the regulation of heat loss. The Journal of physiology, 216(1), 1-10. https://doi.org/10.1113/jphysiol.1971.sp009505
Rostagno, H. S., Albino, L. F. T., Hannas, M. I., Donzele, J. L., Sakomura, N. K., F. G., & Perazzo, B. C. O. (2017). Tabelas Brasileiras para Aves e Suínos: Composição de Alimentos e Exigências Nutricionais (p. 488). UFV.
Sokolowicz, Z., Krawczyk, J., & Dykiel, M. (2018). Effect of alternative housing system and hen genotype on egg quality characteristics. Emirates Journal of Food and Agriculture, 30(8), 695-703. http://dx.doi.org/10.9755/ejfa.2018.v30.i8.1753
Valentim, J. K., Lima, H. J. D., Bittencourt, T. M., Barros, F. K., Costa Braga, J. D., & Antunes, H. C. F. (2019). Performance and welfare of different genetic groups of laying hen. Acta Scientiarum. Animal Science, 41(1), 1-8. http://dx.doi.org/10.4025/actascianimsci.v41i1.42904
Vlckova, J., Tumava, E., Mikova, K. M., Englmaierova, O. M., & Chodova, D. (2019). Changes in the quality of eggs during storage depending on the housing system and the age of hens. Poultry Science, 98(11), 6187-6193. http://dx.doi.org/10.3382/ps/pez401
Weimer, S. L., Robison, C. I., Tempelman, R. J., Jones, D. R., & Karcher, D. M. (2019). Layin hen production and welfare in enriched colony cages at different stocking densities. Poultry Science, 98(9), 3578-3586. http://dx.doi.org/10.3382/ps/pez107
Copyright (c) 2025 Alexander Alexandre de Almeida, Heder José D’Avila Lima, Fernanda Nunes Albernaz Silva, Marcos Vinícius Martins Morais, Debora Duarte Moraleco, Jean Kaique Valentim, Tatiana Marques Bittencourt

This work is licensed under a Creative Commons Attribution 4.0 International License.
DECLARATION OF ORIGINALITY AND COPYRIGHTS
- I Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal.
The copyrights belong exclusively to the authors. Published content is licensed under Creative Commons Attribution 4.0 (CC BY 4.0) guidelines, which allows sharing (copy and distribution of the material in any medium or format) and adaptation (remix, transform, and build upon the material) for any purpose, even commercially, under the terms of attribution.
Read this link for further information on how to use CC BY 4.0 properly.