Comparison of oregano essential oil as antibiotic or probiotic alterative in broilers̓ diet: Use of technique for order of reference by similarity to ideal solution
Abstract
Antibiotic resistance is a major problem for public health worldwide, and nutritionists are searching for antibiotic alternatives. Oregano essential oil is one of the natural products that has antioxidant and antibacterial substances. In the present study the technique of ordering the preference by similarity to the ideal solution was applied to compare different feed additives regarding the biological parameters of broilers fed diets containing antibiotic, probiotic or different levels of oregano essential oil. A total of five hundred Arian broiler chicks were used in a completely randomized design with 5 treatments and 5 replicates. The experimental treatments were: 1) basal diet (control), 2) basal diet+150 ppm antibiotic Avilamycin, 3) basal diet +100 ppm probiotic Protexin, 4) basal diet+200 ppm oregano essential oil, 5) basal diet+400 ppm oregano essential oil. After obtaining data on performance, production index, heterophil to lymphocyte ratio, and ileum microflora of broilers, the multiple attribute decision making (MADM), and the technique for order of preference by similarity to ideal solution (TOPSIS) was applied. Results indicated that oregano essential oil had the potential to be considered as a natural probiotic or antibiotic replacement in broiler chickens̓ diet. Oregano essential oil improved FCR, production index, intestinal villus height/crypt depth of the broilers. Also, it decreased the bacterial count of E.Coli in the ileum content of the broilers. It conclusion using oregano essential oil at the level of 200 or 400 ppm of diet can be considered as probiotic or antibiotic natural alternative in broilers diet.
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Abdel-Latif, H. M., Abdel-Tawwab, M., Khafaga, A. F., & Dawood, M. A. (2020). Dietary oregano essential oil improved the growth performance via enhancing the intestinal morphometry and hepato-renal functions of common carp (Cyprinus carpio L.) fingerlings. Aquaculture, 526, 735432. https://doi.org/10.1016/j.aquaculture.2020.735432
Abou-Elkhair, R., Gaafar, K. M., Elbahy, N. M., Helal, M. A., Mahboub, H. D., & Sameh, G. (2014). Bioactive effect of dietary supplementation with essential oils blend of oregano, thyme and garlic oils on performance of broilers infected with Eimeria species. Global Veterinaria, 13(6), 977-985.
Abu-Lafi, S., Odeh, I., Dewik, H., Qabajah, M., Hanus, L., & Dembitsky, V. (2008). Thymol and carvacrol production from leaves of wild Palestinian Majorana syriaca. Bioresource Technology, 99(9), 3914-3918. https://doi.org/10.1016/j.biortech.2007.07.042
Adaszynska, M., & Szczerbinska, D. (2016). Use of essential oils in broiler chicken production – a review. Annals of Animal Science, 17(2). https://doi.org/10.1515/aoas-2016-0046
Alagawany, M., Abd El-Hack, M. E., Farag, M. R., Shaheen, H. M., Abdel-Latif, M. A., Noreldin, A. E., & Patra, A. K. (2018). The usefulness of oregano and its derivatives in poultry nutrition. World's Poultry Science Journal, 74(3), 463-474. https://doi.org/10.1017/S0043933918000454
Alp, M., Midilli, M., Kocabağlı, N., Yılmaz, H., Turan, N., Gargılı, A., & Acar, N. (2012). The effects of dietary oregano essential oil on live performance, carcass yield, serum immunoglobulin G level, and oocyst count in broilers. The Journal of Applied Poultry Research, 21(3), 630-636. https://doi.org/10.3382/japr.2012-00551
Amad, A. A., Männer, K., Wendler, K. R., Neumann, K., & Zentek, J. (2011). Effects of a phytogenic feed additive on growth performance and ileal nutrient digestibility in broiler chickens. Poultry science, 90(12), 2811-2816. https://doi.org/10.3382/ps.2011-01515
Avila Ramos, F., Pro Martinez, A., Sosa Montes, E., Narciso Gaytan, C., Hernandez Cazarez, A. S., Cibrian Tovar, J., Gallegos Sanchez, J., & Rodriguez Castillo, J. C. (2017). Oregano oil use in broiler diet increases accumulation of carvacrol and thymol in breast meat. Acta Universitaria, 27(1), 34-39. https://doi.org/10.15174/au.2017.1074
Barfourooshi, H. J., Sadeghipanah, H., Asadzadeh, N., Hosseini, S. A., & Alizadeh‐Ghamsari, A. H. (2023). Multi‐attribute decision‐making: Use of scoring methods to choose the best form of dietary fat supplement in pregnant Saanen goats. Veterinary Medicine and Science, 9(6), 2912-2919. https://doi.org/10.1002/vms3.1298
Behnamifar, A., Rahimi, S., Karimi Torshizi, M. A., & Mohammad Zade, Z. (2018). Effect of chamomile, wild mint and oregano herbal extracts on quality and quantity of eggs, hatchability, and some other parameters in laying Japanese quails. Journal of Medicinal Plants and By-products, 7(2), 173-180.
Botsoglou, N. A., Florou-Paneri, P., Christaki, E., Fletouris, D. J., & Spais, A. B. (2002). Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast, thigh and abdominal fat tissues. British poultry science, 43(2), 223-230. https://doi.org/10.1080/00071660120121436
Cheng, C., Xia, M., Zhang, X., Wang, C., Jiang, S., & Peng, J. (2018). Supplementing oregano essential oil in a reduced-protein diet improves growth performance and nutrient digestibility by modulating intestinal bacteria, intestinal morphology, and antioxidative capacity of growing-finishing pigs. Animals, 8(9), 159. https://doi.org/10.3390/ani8090159
Dorman, H. D., & Deans, S. G. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of applied microbiology, 88(2), 308-316. https://doi.org/10.1046/j.1365-2672.2000.00969.x
Eler, G., Gomes, A. V. C., Trindade, B. S., Almeida, L. S. L., Dilelis, F., Cardoso, V. S., & Lima, C. A. R. (2019). Oregano essential oil in the diet of broilers: performance, carcass characteristics, and blood parameters. South African Journal of Animal Science, 49(4), 753-762. https://doi.org/10.4314/sajas.v49i4.17
Giannenas, I., Tzora, A., Sarakatsianos, I., Karamoutsios, A., Skoufos, S., Papaioannou, N., Anastasiou, I., & Skoufos, I. (2016). The effectiveness of the use of oregano and laurel essential oils in chicken feeding. Annals of Animal Science, 16(3), 779-796. https://doi.org/10.1515/aoas-2015-0099
Gul, M., Yilmaz, E., Yildirim, B. A., Sezmis, G. Ü. R. K. A. N., Kaya, A., Timurkaan, S., Onel, S. E., & Tekce, E. (2019). Effects of oregano essential oil (Origanum syriacum L.) on performance, egg quality, intestinal morphology and oxidative stress in laying hens. European Poultry Science/Archiv für Geflügelkunde, 83(290), 1-15. https://doi.org/10.1399/eps.2019.290
Hafeez, A., Manner, K., Schieder, C., & Zentek, J. (2016). Effect of supplementation of phytogenic feed additives (powdered vs. encapsulated) on performance and nutrient digestibility in broiler chickens. Poultry Science, 95(3), 622-629. https://doi.org/10.3382/ps/pev368
Hashemipour, H., Kermanshahi, H., Golian, A., & Veldkamp, T. (2013). Effect of thymol and carvacrol feed supplementation on performance, antioxidant enzyme activities, fatty acid composition, digestive enzyme activities, and immune response in broiler chickens. Poultry science, 92(8), 2059-2069. https://doi.org/10.3382/ps.2012-02685
Hidayat, M. N., Malaka, R., Agustina, L., & Pakiding, W. (2018). Effect of Lactobacillus sp. probiotics on intestinal histology, Escherichia coli in excreta and broiler performance. Journal of the Indonesian Tropical Animal Agriculture, 43(4), 445–452. https://doi.org/10.14710/jitaa.43.4.445-452
Jamroz, D., Wiliczkiewicz, A., Wertelecki, T., Orda, J., & Skorupińska, J. (2005). Use of active substances of plant origin in chicken diets based on maize and locally grown cereals. British poultry science, 46(4), 485-493. https://doi.org/10.1080/00071660500191056
Javaheri Barfourooshi, H., Sadeghipanah, H., Asadzadeh, N., Hosseini, S. A., & Alizadeh‐Ghamsari, A. H. (2023). Multi‐attribute decision‐making: Use of scoring methods to choose the best form of dietary fat supplement in pregnant Saanen goats. Veterinary Medicine and Science, 9(6), 2912-2919. https://doi.org/10.1002/vms3.1298
Johnson, A. M., Anderson, G., Arguelles-Ramos, M., & Ali, A. A. (2022). Effect of dietary essential oil of oregano on performance parameters, gastrointestinal traits, blood lipid profile, and antioxidant capacity of laying hens during the pullet phase. Frontiers in Animal Science, 3, 1072712. https://doi.org/10.3389/fanim.2022.1072712
Khaksar, V., Van Krimpen, M., Hashemipour, H., & Pilevar, M. (2012). Effects of thyme essential oil on performance, some blood parameters and ileal microflora of Japanese quail. The Journal of Poultry Science, 49(2), 106-110. https://doi.org/10.2141/jpsa.011089
Liolios, C. C., Gortzi, O., Lalas, S., Tsaknis, J., & Chinou, I. (2009). Liposomal incorporation of carvacrol and thymol isolated from the essential oil of Origanum dictamnus L. and in vitro antimicrobial activity. Food chemistry, 112(1), 77-83. https://doi.org/10.1016/j.foodchem.2008.05.060
Lohakare, J., & Abdel-Wareth, A. A. (2022). Effects of Dietary Supplementation of Oregano Bioactive Lipid Compounds and Silver Nanoparticles on Broiler Production. Sustainability, 14(21), 13715. https://doi.org/10.3390/su142113715
Lotfollahian, H., Tasharofi, S., Hossieni, S. A., Alizadeh-Ghamsari, A. H., & Tasharofi, S. (2023). Assessing of Peppermint as a Replacement for Antibiotic and Probiotic Using Technique for Order of Preference by Similarity to the Ideal Solution. Iranian Journal of Applied Animal Science, 13(3), 527-534.
Naghdi Badi, H. A., Abdollahi, M., Mehrafarin, A., Ghorbanpour, M., Tolyat, M., Qaderi, A., & Yekta, M. (2017). An Overview on Two Valuable Natural and Bioactive Compounds, Thymol and Carvacrol, in Medicinal Plants. Journal of Medicinal Plants, 16(63), 1-32.
Nilkanth, R. H., Vipin, K. K., & Kumar, P. (2020). Effect of feeding of oregano oil with probiotic on gut microbiota and nutrients digestibility of broiler chicken. Journal of Pharmaceutical Innovation, 9(3), 660-664. https://doi.org/10.22271/tpi.2020.v9.i3l.4657
Peng, Q. Y., Li, J. D., Li, Z., Duan, Z. Y., & Wu, Y. P. (2016). Effects of dietary supplementation with oregano essential oil on growth performance, carcass traits and jejunal morphology in broiler chickens. Animal Feed Science and Technology, 214, 148-153.
Pungky, T. K. A., Endang, S., & Alamsyah, A. (2018). Comparison between SAW and TOPSIS methods in selection of broiler chicken meat quality. Scientific Journal of Informatics, 5(1), 90. https://doi.org/10.15294/sji.v5i1.14416
Ri, C. S., Jiang, X. R., Kim, M. H., Wang, J., Zhang, H. J., Wu, S. G., Bontempo, V., & Qi, G. H. (2017). Effects of dietary oregano powder supplementation on the growth performance, antioxidant status and meat quality of broiler chicks. Italian Journal of Animal Science, 16(2), 246-252. https://doi.org/10.1080/1828051X.2016.1274243
Shroha A (2016). Effect of supplementation of ajwain (Trachyspermum ammi) On the performance of broiler chicken. http://krishikosh.egranth.ac.in/handle /1/5810022625.
Simitzis, P. E. (2017). Enrichment of animal diets with essential oils—a great perspective on improving animal performance and quality characteristics of the derived products. Medicines, 4(2), 35.
Simitzis, P. E., & Deligeorgis, S. G. (2011). The effects of natural antioxidants dietary supplementation on the properties of farm animal products. Animal Feed: Types, Nutrition, Safety. Nova Science Publishers, Inc.
Thompson, K. L., & Applegate, T. J. (2006). Feed withdrawal alters small-intestinal morphology and mucus of broilers. Poultry Science, 85(9), 1535-1540.
Vázquez, R. S., Meléndez, L. A. D., Estrada, E. S., Rodríguez Muela, C., Villalobos, G. V., Zamora, G. M., & Hume, M. E. (2015). Performance of broiler chickens supplemented with Mexican oregano oil (Lippia berlandieri Schauer). Revista Brasileira de Zootecnia, 44(8), 283-289. https://doi.org/10.1590/S1806-92902015000800003
Yazdanpanah Goharrizi, L, & Tasharofi, S. (2017). Inhibiting Aspergillus flavus Growth and Aflatoxin Decrement in Aquatic Feed with Satureja hortensis Essential Oil. Research Journal for Veterinary Practitioners, 4(4), 71-75. http://dx.doi.org/10.14737/journal.rjvp/2016/4.4.71.75
Yousefi, K., Allymehr, M., Talebi, A., & Tukmechi, A. (2024). A comparative study on the expression of myogenic genes, and their effects on performance and meat quality in broiler chicken strains. Veterinary Research Forum, 15(5), 243-250. https://doi.org/10.30466/vrf.2024.2014542.4046
Zhang, L. Y., Peng, Q. Y., Liu, Y. R., Ma, Q. G., Zhang, J. Y., Guo, Y. P., Xue, Z., & Zhao, L. H. (2021). Effects of oregano essential oil as an antibiotic growth promoter alternative on growth performance, antioxidant status, and intestinal health of broilers. Poultry science, 100(7), 101163. http://dx.doi.org/10.1016/j.psj.2021.101163
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