<b>Genetic characterization of three Nile tilapia (GIFT strain) generations using the RAPD marker</b> - doi: 10.4025/actascianimsci.v33i2.9202

  • Sheila Nogueira Oliveira Universidade Estadual de Maringa
  • Ricardo Pereira Ribeiro Universidade Estadual de Maringa
  • Nelson Mauricio Lopera Universidade Estadual de Maringa
  • Fernanda Braz Candioto Universidade Estadual de Maringa
  • Emiko Kawakami Resende EMBRAPA
  • Angela Puchinik Legat EMBRAPA
Keywords: genetic diversity, Genetic Improvement of Farmed Tilapias, strains, genetic improvement, Oreochromis niloticus

Abstract

The aim of this work was to analyze the genetic diversity in three GIFT tilapia generations from the Genetic Improvement Program of State University of Maringá, using the RAPD marker. One hundred eighty fin samples were used. The seven primers used produced 91 fragments, of which 98.9% were polymorphic. Differences (p < 0.05) were observed in the frequency of 64 fragments, of which ten were excluded in G2. The results of genetic variability estimated by the Shannon index (G0: 0.430, G1: 0.469 and G2: 0.420) and by the percentage of polymorphic fragments (G0: 90.1%, G1: 94.5% and G2: 86.8%) indicated a high intrapopulation genetic variability, corroborated by the Nei genetic diversity results that showed G1 with the highest values (G0: 0.281, G1: 0.307 and G2: 0.277). A greater genetic distance between the G0 and G1 generations (0.108) and a greater genetic identity between G0 and G2 (0.909) were verified. Intrapopulation genetic diversity was high in the three generations, with highest values in G1.

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Author Biography

Sheila Nogueira Oliveira, Universidade Estadual de Maringa
Zootecnisca formada na Universidade Estadual de Maringa, aluna do programa de pós graduação PPZ/UEM na grande área de produção animal/Piscicultura, e especificamente na área de Melhoramento Genético Animal. Departamento de Zootecnia - área Piscicultura.
Published
2011-04-28
How to Cite
Oliveira, S. N., Ribeiro, R. P., Lopera, N. M., Candioto, F. B., Resende, E. K., & Legat, A. P. (2011). <b>Genetic characterization of three Nile tilapia (GIFT strain) generations using the RAPD marker</b&gt; - doi: 10.4025/actascianimsci.v33i2.9202. Acta Scientiarum. Animal Sciences, 33(2), 207-212. https://doi.org/10.4025/actascianimsci.v33i2.9202
Section
Animal Breeding and Reproduction

0.9
2019CiteScore
 
 
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