<b>Non-invasive method for buffalo DNA extraction</b> - DOI: 10.4025/actascianimsci.v30i4.4839

Abstract

The objective of this paper was to compare two different protocols for DNA extraction from buffalo (Bubalus bubalis) fur. Also, three sites for the fur source (back of the head, right shoulder and forehead) were compared. For the experiments, four animals were used and three replicates for each site on each animal were performed. For protocol 1, the phenol chloroform technique was used, and the CTAB extraction technique was used for protocol 2. Protocol 2 resulted in a higher average of DNA concentration for the fur samples. Considering the body region from where the fur was extracted, there were no significant differences in DNA concentration. However, the forehead showed a higher concentration when extracted with CTAB. Considering the ease of use for each protocol, protocol 2, in addition to presenting greater concentrations of DNA, took less time to be performed — three hours and fifty minutes, and did not require another toxic reagent, such as phenol. For this reason, it is recommended that the material be extracted from the forehead, asit is an easy site to access the fur. The recommended protocol for DNA extraction is with CTAB, because of its practicality and lower execution time.