Effects of a monocomponent protease from Bacillus licheniformis on broiler performance, digestibility, and carcass yield

Palavras-chave: additive; exogenous enzyme; nutrition; poultry.

Resumo

Two experiments were carried out to evaluate the effects of protease addition to the diet of broilers at a higher level (1× or 2×) than the nutritional value proposed for the enzyme. The first experiment, 1280 day-old chicks (Cobb500®) were randomly allocated (randomized block design, 2×2+1 factorial arrangement), five treatments, eight replicates containing 32 birds/replicate. Treatments consisted: control diet without protease (CD); CD + 1× nutritional value of the enzyme (CDM1); CD + 2× nutritional value of the enzyme (CDM2); CDM1 + protease; and CDM2 + protease. The experimental period was 42 days. The mean weight (AFW), feed intake (FI), weight gain (WG), feed conversion, and carcass yield were evaluated. Significant differences were observed for AFW, WG, FI, abdominal fat yield, and feet percentage in the carcass. In the second experiment, 120 Cobb500® chicks at 14 days of age were allotted in a completely randomized design, 2×2+1 factorial arrangement, five treatments, six replicates with four birds/replicate. The treatments were consistent with the first experiment. Significant improvements in the nitrogen balance were observed for the broilers that received protease. The use of the enzyme tested is recommended with the recommended nutritional matrix, improving the zootechnical indices of broilers.

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Referências

Angel, C. R., Saylor, W., Vieira, S. L., & Ward, N. (2011). Effects of a monocomponent protease on performance and protein utilization in 7- to 22-day-old broiler chickens. Poultry Science, 90(10), 2281-2286. DOI: http://dx.doi.org/10.3382/ps.2011-01482

Associação Brasileira de Proteina Animal [ABPA]. (2021). Relatório Anual 2021. Retrieved from http://abpa-br.org/wp-content/uploads/2021/04/ABPA_Relatorio_Anual_2021_web.pdf

Batal, A. B., & Parsons, C. M. (2002). Effects of age on nutrient digestibility in chicks fed different diets. Poultry Science, 81(3), 400-407. DOI: http://dx.doi.org/10.1093/ps/81.3.400

Cardinal, K. M., Moraes, M. L., Andretta, I., Schirmann, G. D., Belote, B. L., Barrios, M. A., … Ribeiro, A. M. L. (2019). Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease. Revista Brasileira de Zootecnia, 48(e20180232), 1-11. DOI: http://dx.doi.org/10.1590/rbz4820180232

Cardoso, D. M., Maciel, M. P., Passos, D. P., Silva, F. V., Reis, S. T., & Aiura, F. S. (2011). Efeito do uso de complexo enzimático em rações para frangos de corte. Archivos de Zootecnia, 60(232), 1053-1064. DOI: http://dx.doi.org/10.4321/S0004-05922011000400021

Carvalho, D. P., Leandro, N. S. M., Andrade, M. A., Oliveira, H. F., Pires, M. F., Teixeira, K. A., ... Stringhini, J. H. (2020). Protease inclusion in plant- and animal-based broiler diets: performance, digestibility and biometry of digestive organs. South African Journal of Animal Science, 50(2), 291-301. DOI: http://dx.doi.org/10.4314/sajas.v50i2.12

Dalólio, F. S., Moreira, J., Vaz, D. P., Albino, L. F. T., Valadares, L. R., Pires, A. V., & Pinheiro, S. R. F. (2016). Exogenous enzymes in diets for broilers. Revista Brasileira de Saúde e Produção Animal, 17(2), 149-161. DOI: http://dx.doi.org/10.1590/S1519-99402016000200003

Dosković, V., Bogosavljević-Bošković, S., Pavlovski, Z., Milošević, B., Škrbić, Z., Radonjac, S., & Petričević, V. (2012). The effect of protease on productive and slaughter traits in broiler chickens. Biotechnology in Animal Husbandry, 28(4), 817-826. DOI: http://dx.doi.org/10.2298/BAH1204817D

Freitas, D. M. S. L., Vieira, C. R., Angel, A., Favero, A., & Maiorka, A. (2011). Performance and nutrient utilization of broilers fed diets supplemented with a novel monocomponent protease. Journal of Applied Poultry Research, 20(3), 322-334. DOI: http://dx.doi.org/10.3382/japr.2010-00295

Gomide, E. M., Rodrigues, P. B., Freitas, R. T. F., & Fialho, E. T. (2007). Planos nutricionais com a utilização de aminoácidos e fitase para frangos de corte mantendo o conceito de proteína ideal nas dietas. Revista Brasileira de Zootecnia, 36(6), 1769-1774. DOI: http://dx.doi.org/10.1590/S1516-35982007000800009

Law, F. L., Zulkifli, I., Soleimani, A. F., Liang, J. B., & Awad, E. A. (2018). The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment. Asian-Australasian Journal of Animal Science, 31(8), 1291-1300. DOI: http://dx.doi.org/10.5713/ajas.17.0581

Leinonen, I., & Williams, A.G. (2015). Effects of dietary protease on nitrogen emissions from broiler production: a holistic comparison using life cycle assessment. Journal of The Science of Food and Agriculture, 95(15), 3041-3046. DOI: http://dx.doi.org/10.1002/jsfa.7202

Leite, P. R. S. C., Leandro, N. S. M., Stringhini, J. H., Café, M. B., Gomes, N. A., & Jardim Filho, R. M. (2011). Desempenho de frangos de corte e digestibilidade de rações com sorgo ou milheto e complexo enzimático. Pesquisa Agropecuária Brasileira, 46(3), 280-286. DOI: http://dx.doi.org/10.1590/S0100-204X2011000300008

Lourenco, J. M., Nunn, S. C., Lee, E. J., Dove, R., Callaway, T. R., & Azain, M. J. (2020). Effect of supplemental protease on growth performance and excreta microbiome of broiler chicks. Microorganisms, 8(4), 1-14. DOI: http://dx.doi.org/10.3390/microorganisms8040475

Matias, C. F. Q., Rocha, J. S. R., Pompeu, M. A., Baião, R. C., Baião, N. C., Lara, L. J. C., ... Cardeal, P. C. (2015). Efeito da protease sobre o coeficiente de metabolizabilidade dos nutrientes em frangos de corte. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 67(2), 492-498. http://dx.doi.org/10.1590/1678-7883

Matterson, L. D., Potter, L. M., Stutz, M. W., & Singsen, E. P. (1965). The metabolizable energy of feeds ingredients for chickens. Agricultural Experiment Station Research Report, 7, 1-11. DOI: https://www.cabdirect.org/cabdirect/abstract/19671403742

Miranda, L. M. B., Goulart, C. C., Leite, S. C. B., Batista, A. S. M., & Lima, R. C. (2017). Farelo de algodão em dietas com ou sem suplementação de enzimas para frangos de corte. Revista Ciência Agronômica, 48(4), 690-699. DOI: http://dx.doi.org/10.5935/1806-6690.20170080

Moura, F. A. S., Dourado, L. R. B., Farias, L. A., Lopes, J. B., Lima, S. B. P., & Fernandes, M. L. (2019). Complexos enzimáticos sobre a energia metabolizável e digestibilidade dos nutrientes do milheto para frangos de corte. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 71(3), 990-996. DOI: http://dx.doi.org/10.1590/1678-4162-10021

Noy, Y., & Sklan, D. (2002). Nutrient use in chicks during the first week posthatch. Poultry Science, 81(3), 391-399. DOI: http://dx.doi.org/10.1093/ps/81.3.391

Oxenboll, K. M., Pontopiddan, K., & Fru-Nji, F. (2011). Use of a protease in poultry feed offers promising environmental benefits. International Journal of Poultry Science, 10(11), 842-848. DOI: http://dx.doi.org/10.3923/ijps.2011.842.848

Ribeiro, J. S., Fassani, E. J., Makiyama, L., & Clemente, A. H. S. (2015). Suplementação de enzimas amilase, fitase e protease para codornas japonesas em postura. Boletim de Indústria Animal, 72(2), 163-169. DOI: http://dx.doi.org/10.17523/bia.v72n2p163

Sakomura, N. K., & Rostagno, H. S. (2016). Métodos de pesquisa em nutrição de monogástricos. São Paulo, SP: Funep.

Scotta, B. A., Albino, L. F. T., Brustolini, P. C., Gomide, A. P. C., Campos, P. F., & Rodrigues, V. V. (2016). Determinação da composição química e dos valores de energia metabolizável de alguns alimentos proteicos para frangos de corte. Ciência Animal Brasileira, 17(4), 501-508. DOI: http://dx.doi.org/10.1590/1089-6891v17i421347

Silva, D. J., & Queiroz, A. C. (2002). Análise de alimentos (métodos químicos e biológicos). Viçosa, MG: Universidade Federal de Viçosa.

Sklan, D., & Noy, Y. (2004). Catabolism and deposition of amino acids in growing chicks: effect of dietary supply. Poultry Science, 83(6), 952-961. DOI: http://dx.doi.org/10.1093/ps/83.6.952

Toghyani, M., Swick, R. A., & Barekatain, R. (2017). Effect of seed source and pelleting temperature during steam pelleting on apparent metabolizable energy value of full-fat canola seed for broiler chickens. Poultry Science, 96(5), 1325-1333. DOI: http://dx.doi.org/10.3382/ps/pew401

Vasconcellos, C. H. F., Fontes, D. O., Lara, L. J. C., Vidal, T. Z. B., Silva, M. A., & Silva, P. C. (2011). Determinação da energia metabolizável e balanço de nitrogênio de dietas com diferentes teores de proteína bruta para frangos de corte. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 63(3), 659-669. DOI: http://dx.doi.org/10.1590/S0102-09352011000300018

Walk, C. L., Juntunen, K., Paloheimo, M., & Ledoux, D. R. (2019). Evaluation of novel protease enzymes on growth performance and nutriente digestibility of poultry: enzyme dose response. Poultry Science, 98(11), 5525-5532. DOI: http://dx.doi.org/10.3382/ps/pez299

Yuan, L., Wang, S. Q., Wang, Z. X., Zhu, H., & Huang, K. (2015). Effects of exogenous protease supplementation on endogenous trypsin activity and gene expression. in broilers. Genetics and Molecular Research, 14(4), 13633-13641. DOI: http://dx.doi.org/10.4238/2015.October.28.25

Publicado
2022-12-09
Como Citar
Carneiro, A. L. M., Café, M. B., Carvalho, F. B. de, Oliveira, H. F. de, Carvalho, D. P., Carvalho, G. B. de, Gomides, L. P. S., & Stringhini, J. H. (2022). Effects of a monocomponent protease from Bacillus licheniformis on broiler performance, digestibility, and carcass yield. Acta Scientiarum. Animal Sciences, 45(1), e58499. https://doi.org/10.4025/actascianimsci.v45i1.58499
Seção
Nutrição de Não-Ruminantes

0.9
2019CiteScore
 
 
29th percentile
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