<b>Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (<i>Bombyx mori</i> Lineu, 1758) and infected with nucleopolyhedrovirus</b> - doi: 10.4025/actascibiolsci.v35i1.8612

  • Juliana Bueno Ruiz Universidade Estadual de Maringá
  • Ludimilla Ronqui Universidade Federal de Rondonia
  • Jussara Ricardo de Oliveira Universidade Paranaense
  • Maria Claudia Colla Ruvolo-Takasusuki Universidade Estadual de Maringá
Palavras-chave: viral infection, silk gland, isoenzymes, SDS-PAGE, molecular markers

Resumo

Total proteins and esterases from silk gland extracts of Bombyx mori silkworm were characterized and compared with electrophoretic profiles of prepared extracts with silkworm glands infected with nucleopolyhedrovirus (BmNPV). SDS-PAGE (7%) gels was used for the total proteins, and 10% native PAGE for esterases. In the silk glands extracts of healthy silkworms, it was observed seven protein zones with molecular weight varying between 10 kDa (P1) and 60 kDa (P7). In the infected silkworms, a new zone named P8 (90 kDa) was also detected. Esterases activity at 5th instar larvae underwent changes after the infection with BmNPV, since there was a reduction (EST-6 and EST-7) and an increase (EST-8) in the intensity of the regions of esterases activity, and specificity of EST-9 to β-naphthyl acetate. Those alterations observed in the expression of genes after the infection with the nucleoplyhedrovirus can be used as markers to detect infections in B. mori.


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Biografia do Autor

Maria Claudia Colla Ruvolo-Takasusuki, Universidade Estadual de Maringá
Atualmente é PROFESSOR ASSOCIADO-NÍVEL C da Universidade Estadual de Maringá. Tem experiência na área de Genética , com ênfase em Genética Animal. Atuando principalmente nos seguintes temas: esterases, Apis mellifera, Caracterizacao Genetica, Expressão tecidual, Expressao No Desenvolvimento. Currículo Lattes
Publicado
2012-07-19
Como Citar
Ruiz, J. B., Ronqui, L., Oliveira, J. R. de, & Ruvolo-Takasusuki, M. C. C. (2012). <b>Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (<i>Bombyx mori</i> Lineu, 1758) and infected with nucleopolyhedrovirus</b&gt; - doi: 10.4025/actascibiolsci.v35i1.8612. Acta Scientiarum. Biological Sciences, 35(1), 77-82. https://doi.org/10.4025/actascibiolsci.v35i1.8612
Seção
Genética

 

0.6
2019CiteScore
 
 
31st percentile
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0.6
2019CiteScore
 
 
31st percentile
Powered by  Scopus