Evaluation of the semen characteistics after induced spermiation in the bullfrog Lithobates catesbeianus - doi: 10.4025/actascibiolsci.v35i3.14780

Marcelo Maia Pereira; Oswaldo Pinto Ribeiro Filho; Jose Cola Zanuncio; Rodrigo Diana Navarro; Jose Teixeira Seixas Filho; Christiano Demétrio  de Lima Ribeiro

doi:10.4025/actascibiolsci.v35i3.14780

Evaluation of the semen characteistics after induced spermiation in the bullfrog Lithobates catesbeianus - doi: 10.4025/actascibiolsci.v35i3.14780

Marcelo Maia Pereira Universidade Federal de Viçosa
Oswaldo Pinto Ribeiro Filho Universidade Federal de Viçosa.
Jose Cola Zanuncio Universidade Federal de Viçosa
Rodrigo Diana Navarro Universidade de Brasília
Jose Teixeira Seixas Filho Fundação Instituto de Pesca do Rio de Janeiro
Christiano Demétrio de Lima Ribeiro Universidade Federal de Viçosa

DOI: https://doi.org/10.4025/actascibiolsci.v35i3.14780

Abstract

Evaluation of semen characteristics after hormonal induction of the bullfrog could provide valuable information on the gametes of this species, which may be useful for projects related to artificial fertilization, animal improvement, and cryopreservation. Bullfrog males were induced to spermiate with buserelin acetate (GnRHa), and their semen was subsequently analyzed. GnRHa (0.4 μg) was administered to the bullfrog males with secondary sexual characteristics such as weight > 200 g, yellow chin, nuptial callus, and amplexus reflex, being the semen collected after 60 min. The semen volume was 5.76 mL, light-colored. The other characteristics of the semen were: vigor of 4.80, motility of 93%, concentration of 14.24 × 10⁶ mL^-1, and content of normal spermatozoa of 70%. The volume, color, vigor, motility, sperm concentration, and content of normal spermatozoa were adequate in these bullfrog semen samples. Evaluation of the bullfrog semen samples based on this set of parameters is essential for decision-making about the quality and destination of the semen.

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Author Biographies

Marcelo Maia Pereira, Universidade Federal de Viçosa

Eng. Agronomo formado pela UFV e Mestre em Biologia Animal. Doutorando do CAUNESP.

Oswaldo Pinto Ribeiro Filho, Universidade Federal de Viçosa.

Porfessor do Departamento de Biologia Animal, Universidade Federal de Viçosa.

Jose Cola Zanuncio, Universidade Federal de Viçosa

Porfessor do Departamento de Biologia Animal, Universidade Federal de Viçosa.

Rodrigo Diana Navarro, Universidade de Brasília

Professor de Aquicultura da UnB.

Jose Teixeira Seixas Filho, Fundação Instituto de Pesca do Rio de Janeiro

Pesquisador em Nutrição de Organismos Aquáticos Fundação Instituto de Pesca do Rio de Janeiro - FIPERJ

Christiano Demétrio de Lima Ribeiro, Universidade Federal de Viçosa

Biológo, Mestre em Biologia Animal.

Published

2013-02-28

How to Cite

Pereira, M. M., Ribeiro Filho, O. P., Zanuncio, J. C., Navarro, R. D., Seixas Filho, J. T., & Ribeiro, C. D. de L. (2013). Evaluation of the semen characteistics after induced spermiation in the bullfrog Lithobates catesbeianus - doi: 10.4025/actascibiolsci.v35i3.14780. Acta Scientiarum. Biological Sciences, 35(3), 305-310. https://doi.org/10.4025/actascibiolsci.v35i3.14780

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Issue

Vol 35 No 3 (2013)

Section

Aquiculture and Fisheries Resources

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