Produção e caracterização de um marcador potencial de linfócitos T em surubim híbrido (Pseudoplatystoma corruscans X P. fasciatum) - DOI: 10.4025/actascibiolsci.v26i2.1636

Roger Beelen; Gilberto Cezar Pavanelli; Bobbie Boyd; Julio Cesar Garavello; Patricia Mendes Guimarães Beelen; Avery Jerald Ainsworth

doi:10.4025/actascibiolsci.v26i2.1636

Production and characterization of a putative T-cell marker in hybrid surubim catfish Pseudoplatystoma corruscans (Agassiz) x Pseudoplatystoma fasciatum (Linnaeus) - DOI: 10.4025/actascibiolsci.v26i2.1636

Roger Beelen UFPA
Gilberto Cezar Pavanelli UEM
Bobbie Boyd Mississippi State University
Julio Cesar Garavello Universidade Federal de São Carlos
Patricia Mendes Guimarães Beelen UFPA
Avery Jerald Ainsworth Mississippi State University

DOI: https://doi.org/10.4025/actascibiolsci.v26i2.1636

Abstract

A monoclonal antibody against hybrid surubim thymocytes and circulating T-cells (PST 33) was produced and characterized. The mAb was obtained by immunizing mice with isolated membrane molecules of hybrid surubim thymocytes, and whole thymocytes. Flow cytometric analysis showed that mAb PST 33 reacted with 87.5% of the thymocytes, 34.1% of circulating, 19.1% of pronephros, and 36.6% of spleen lymphoid cells. Immunohistochemistry demonstrated that PST 33-immunoreactive cells are mostly present in the medullar region of the thymus, and in the vicinity of arterioles spread throughout the parenchyma of the spleen. PST 33 appeared as a suitable marker for immunohistochemistry, and should prove useful in studying the ontogeny of surubim immune system. Immunogold labeling revealed that PST 33+ cells were lymphoid-like cells. Morphologically they appeared as small lymphocytes with a high nucleus/cytoplasm ratio. Western blotting of non-reduced membrane lysates showed that PST 33 reacted with a 215 kDa molecule

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Author Biography

Roger Beelen, UFPA

Possui graduação em Zootecnia pela Universidade Federal do Rio Grande do Norte (1993), mestrado em Sciences Naturelles Appliquées - Universite Catholique de Louvain, Bélgica (1995) e doutorado em Ciências, Ecologia e Recursos Naturais pela Universidade Federal de São Carlos (2002), com doutorado sanduíche na Mississippi State University (EUA). Atualmente é professor adjunto da Universidade Federal de Alagoas. Tem experiência na área de Zootecnia, com ênfase em apicultura e piscicultura, atuando principalmente nos seguintes temas: produção de apis mellifera, produção e sanidade de peixes e avaliação de alimentos para animais Currículo Lattes

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Published

2008-04-01

How to Cite

Beelen, R., Pavanelli, G. C., Boyd, B., Garavello, J. C., Beelen, P. M. G., & Ainsworth, A. J. (2008). Production and characterization of a putative T-cell marker in hybrid surubim catfish Pseudoplatystoma corruscans (Agassiz) x Pseudoplatystoma fasciatum (Linnaeus) - DOI: 10.4025/actascibiolsci.v26i2.1636. Acta Scientiarum. Biological Sciences, 26(2), 201-207. https://doi.org/10.4025/actascibiolsci.v26i2.1636

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Issue

Vol 26 No 2 (2004)

Section

Biology Sciences

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