<em>In vitro</em> propagation of <em>Ocimum basilicum</em> L. (Lamiaceae)
Abstract
Ocimum basilicum L. (sweet basil), of the family Lamiaceae, is rich in aromatic essential oils and valuable for its pharmaceutical, aromatic and culinary properties. The present study describes the procedure for micropropagation of O. basilicum using cotiledonary leafs from in vitro geminated plants. Cotyledons from in vitro germinated seeds were used as initial explants, put in MS (Murashige and Skoog, 1962) medium with 0.2mg.L-1 NAA (1-Naphthalene acetic acid) in combination with 0–5mg.L-1 BAP(6-Benzyl aminopurine) and kept at 28 ± 1ºC, 16-h light photoperiod and 48µmol.m-2.s-1 luminous density flow, for 45 days. The highest efficiency of shoot formation after 45 days occurred in the medium containing 5mg.L-1 BAP and 0,2mg.L-1 NAA. The presence of NAA inhibited root formation, when combined with different concentrations of cytokinin (BAP, 1 to 5mg.L-1). Higher BAP concentrations induced an increase in the number of explants with shoots and a higher number of shoots/cotyledonDownloads
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Published
2008-04-17
How to Cite
Dode, L. B., Bobrowski, V. L., Braga, E. J. B., Seixas, F. K., & Schuch, M. W. (2008). <em>In vitro</em> propagation of <em>Ocimum basilicum</em> L. (Lamiaceae). Acta Scientiarum. Biological Sciences, 25(2), 435-437. https://doi.org/10.4025/actascibiolsci.v25i2.2034
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Section
Biology Sciences
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0.6
2019CiteScore
31st percentile
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0.6
2019CiteScore
31st percentile
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